Anti Acetylaminofluorene (AAF) DNA Adducts mAb (Clone AAF-1)

Cosmo Bio

SKU:
CAC-NM-MA-001
  • Figure 1. AAF-DNA adducts recognized by AAF-1.
  • Figure 2. Dose-dependent formation of NA-AAF-induced DNA adducts in human cells. Cells were exposed to NA-AAF for 0.5 h and the formation of DNA adducts in denatured DNA (500 ng/well) was determined using a sensitive-direct-binding ELISA with AAF-1 (1/100). (Details are shown in Ref. 3)
  • Figure 3. The formation of NA-AAF-induced DNA adducts in human cells. Cells were exposed to 200 μM NA-AAF or solvent for 0.5 h. After permeabilization and fixation, DNA adducts were visualized by sequential treatment with AAF-1 (1/25) and ALEXA FLUOR® 488 goat anti-mouse IgG conjugate. Nuclear DNA was counterstained with DAPI.
Shipping:
Calculated at Checkout
$550.00
Adding to cart… The item has been added

DNA adducts in mammalian cells exposed to N-acetoxy-2-acetylaminofluorene (NA-AAF), an activated derivative of the potent carcinogen 2-AAF, play significant roles in cell killing, chromosome aberration, gene mutation and neoplastic transformation. NA-AAF binds covalently to guanine in the DNA of mammalian cells and produces three different DNA adducts. The C-8 adducts dG-C8-AAF and deacetylated dG-C8-AF account for the major portion of the DNA-bound products, while the minor N2 adduct dG-N2-AAF accounts for the remainder. The relative induction levels of the two major C-8 adducts vary among cell types. These adducts distort the DNA helix and therefore are repaired by nucleotide excision repair in human cells. Our AAF-1 antibodies bind most efficiently to dG-C8-AAF and less efficiently to dG-C8-AF in denatured DNA. The antibodies enable one to detect AAF-DNA adducts in DNA from cultured cells using an enzyme-linked immunosorbent assay (ELISA) and to visualize them in cultured cells or rodent tissues by immunofluorescence (IF). This technology will contribute to understanding of molecular mechanisms in AAF-related research fields including cancer research, anticancer research and toxicology.

Source: Toshio Mori Professor, Research Institute for Advanced Medicine, Nara Medical University.

References:
1) R.H. Heflich and R.E. Neft, Genetic toxicity of 2-acetylaminofluorene, 2-aminofluorene and some of their metabolites and model metabolites. Mutation Res. 318 (1994) 73-174.
2) E.Kriek, Fifty years of research on N-acetyl-2-aminofluorene, one of the most versatile compounds in experimental cancer research. J. Cancer Res. Clin. Oncol. 118 (1992) 481-489.
3) T. Iwamoto et al., In situ detection of acetylaminofluorene-DNA adducts in human cells using monoclonal antibodies. DNA Repair 3 (2004) 1475-1482.



Product Specifications
Application ICC, ELISA, IHC, IF
Reactivity All
Clonality Monoclonal (Clone No.: AAF-1)
Host Mouse


Documents & Links for Anti Acetylaminofluorene (AAF) DNA Adducts mAb (Clone AAF-1)
Datasheet cac-nm-ma-001_anti-aaf-dna-adducts-mab-clone-aaf-1_datasheet.pdf

Documents & Links for Anti Acetylaminofluorene (AAF) DNA Adducts mAb (Clone AAF-1)
Datasheet cac-nm-ma-001_anti-aaf-dna-adducts-mab-clone-aaf-1_datasheet.pdf



!