Alkaline Phosphatase Staining Kit

Cosmo Bio LTD

Catalog No: PMC-AK20

Click here to browse a well organized list of products for Bone, Collagen, and Extracellular Matrix research.

Please click here to view other Bone-related Products

Click here to view our exclusive collection of PMC products. 

Alkaline Phosphatase Staining Kit is designed for the rapid and sensitive detection of osteoblasts differentiated in culture from bone marrow cells. Bone forming osteoblasts and bone-degrading osteoclasts can be distinguished by the expression, respectively, of alkaline phosphatase and tartrate-resistant acid phosphatase (TRAP). CosmoBio’s TRAP staining kit (PMC-AK04F-COS) is ideal for rapid and convenient detection of the latter.

Simple staining protocol

1. Remove culture medium. Wash each well 3 times with 1mL PBS.
2. Add 500μL of 10% formalin neutral buffer solution to each well and fix for 20 minutes at room temperature.
3. Remove 10% formalin neutral buffer solution and wash each well 3 times with 2mL deionized water.
4. Dissolve 1 vial of Chromogenic Substrate in 5mL Substrate-containing Buffer.
5. Add 400μL Chromogenic Substrate to each well.
6. Incubate at 37°C for 5-20 minutes. Adjust incubation time until stained.
7. Wash with deionized water to stop the reaction.

References

1. A. S. H. De Jong, M. Van Kessel-Van Vark and A. K. Raap. Sensitivity of various visualization methods for peroxidase and alkaline phosphatase activity in immunoenzyme histochemistry. The Histochemical Journal Volume 17, 1119-1130 (1985)

Citations for Alkaline Phosphatase Staining Kit – 7 Found

Linh, Nguyen Tb; Paul, Kallyanashis; Kim, Boram; Lee, Byong-Taek. Augmenting in vitro osteogenesis of a glycine-arginine-glycine-aspartic-conjugated oxidized alginate-gelatin-biphasic calcium phosphate hydrogel composite and in vivo bone biogenesis through stem cell delivery. Journal Of Biomaterials Applications. 2016;31(5):661-673. 
- Comments: Cosmo Bio’s Alkaline Phosphatase (ALP) Staining Kit was used to detect osteoblastic activity in a study showing Ebag9-knockout mice exhibit fragile bones with low bone mineral density due to decreased bone formation and enhanced bone resorption arising from a role with interacting protein TMSF1 in osteoblast and osteoclast autophagy.

Ali, Manahil; Okamoto, Motoki; Komichi, Shungo; Watanabe, Masakatsu; Huang, Hailing; Takahashi, Yusuke; Hayashi, Mikako. Lithium-containing surface pre-reacted glass fillers enhance hDPSC functions and induce reparative dentin formation in a rat pulp capping model through activation of Wnt/β-catenin signaling. Acta Biomaterialia. 2019;96( 31212112):594-604. 
- Comments: Cosmo Bio’s Alkaline Phosphatase (ALP) Staining Kit was used to detect osteoblastic activity in a study showing Ebag9-knockout mice exhibit fragile bones with low bone mineral density due to decreased bone formation and enhanced bone resorption arising from a role with interacting protein TMSF1 in osteoblast and osteoclast autophagy.

Azuma, Kotaro; Ikeda, Kazuhiro; Shiba, Sachiko; Sato, Wataru; Horie, Kuniko; Hasegawa, Tomoka; Amizuka, Norio; Tanaka, Shinya; Inoue, Satoshi. EBAG9-deficient mice display decreased bone mineral density with suppressed autophagy. Iscience. 2024;27(2):108871. 
- Comments: Cosmo Bio’s Alkaline Phosphatase (ALP) Staining Kit was used to detect osteoblastic activity in a study showing Ebag9-knockout mice exhibit fragile bones with low bone mineral density due to decreased bone formation and enhanced bone resorption arising from a role with interacting protein TMSF1 in osteoblast and osteoclast autophagy.

Sirisereephap, Kridtapat; Tamura, Hikaru; Lim, Jong-Hyung; Surboyo, Meircurius Dwi Condro; Isono, Toshihito; Hiyoshi, Takumi; Rosenkranz, Andrea L; Sato-Yamada, Yurie; Domon, Hisanori; Ikeda, Akari; Hirose, Tomoyasu; Sunazuka, Toshiaki; Yoshiba, Nagako; Okada, Hiroyuki; Terao, Yutaka; Maeda, Takeyasu; Tabeta, Koichi; Chavakis, Triantafyllos; Hajishengallis, George; Maekawa, Tomoki. A novel macrolide-Del-1 axis to regenerate bone in old age. Iscience. 2024;27(2):108798. 
- Comments: Cosmo Bio’s Alkaline Phosphatase (ALP) Staining Kit was used to detect osteoblastic activity in a study revealing a macrolide–DEL-1 axis that can regenerate bone loss due to aging-related disease.

Takara, Kenyo; Maruo, Naoki; Oka, Kyoko; Kaji, Chiaki; Hatakeyama, Yuji; Sawa, Naruhiko; Kato, Yukinari; Yamashita, Junro; Kojima, Hiroshi; Sawa, Yoshihiko. Morphological study of tooth development in podoplanin-deficient mice. Plos One. 12(2):e0171912. 
- Comments: Cosmo Bio’s Alkaline Phosphatase Staining Kit was used in an electron microscopic study of podoplanin conditional KO mice, revealing no effect on the development and growth of bones and teeth (including on calcification and alkaline phosphatase activity in cultured calvarial osteoblasts), but a possible effect on formation of the osteocyte network created by cell process elongation.

Wu, Peizheng; Yanagi, Kazuya; Yokota, Kazuki; Hakamada, Masataka; Mabuchi, Mamoru. Unusual effects of a nanoporous gold substrate on cell adhesion and differentiation because of independent multi-branch signaling of focal adhesions. Journal Of Materials Science. Materials In Medicine. 2023;34(11):54. 
- Comments: Cosmo Bio’s Alkaline Phosphatase Staining Kit was used in a study of the effects of nanoporous gold substrates with different pore sizes on cell adhesion and osteogenic and adipogenic differentiation.

Sirisereephap, Kridtapat; Surboyo, Meircurius Dwi Condro; Rosenkranz, Andrea L; Terao, Yutaka; Tabeta, Koichi; Maeda, Takeyasu; Hajishengallis, George; Maekawa, Tomoki. Protocols for collecting mouse PDL cells and bone marrow cells, differentiation, and data analysis. Star Protocols. 2024;5(3):103162.