Next-generation RACE
In RACE (Rapid Amplification of cDNA Ends) by the single-primer method* of this kit, the targeted cDNA is amplified by PCR with only a gene-specific primer using ds cDNA as a template.
The mechanism is based on that the terminal region of the ds cDNA is partially denatured at 68 C for the extension reaction and that the linear DNA molecule tends to circularize.
Upon reaching the 5' end of the template DNA, thermostable DNA polymerase switches templates to the 5' terminal region of the newly synthesized daughter strand at a certain probability and synthesizes DNA sequences complementary to the gene-specific primer.
Using this daughter strand as a template, the targeted cDNA is amplified with only a gene- specific primer.
Feature and Advantages: By using the accura-expRACE KIT, both 5' and 3' RACE can easily and efficiently be performed under simple conditions, such as RT-PCR.
Long cDNA and rare cDNA unidentified previously can be isolated.
The synthesized ds cDNA can be used as a cDNA library. You can perform ~400 screenings of a cDNA library by RACE.
The cDNA synthesized by this kit contains a high proportion of full-length cDNA because an "advanced type M-MLV Reverse Transcriptase" is used.
The mechanism is based on that the terminal region of the ds cDNA is partially denatured at 68 C for the extension reaction and that the linear DNA molecule tends to circularize.
Upon reaching the 5' end of the template DNA, thermostable DNA polymerase switches templates to the 5' terminal region of the newly synthesized daughter strand at a certain probability and synthesizes DNA sequences complementary to the gene-specific primer.
Using this daughter strand as a template, the targeted cDNA is amplified with only a gene- specific primer.
Feature and Advantages: By using the accura-expRACE KIT, both 5' and 3' RACE can easily and efficiently be performed under simple conditions, such as RT-PCR.
Long cDNA and rare cDNA unidentified previously can be isolated.
The synthesized ds cDNA can be used as a cDNA library. You can perform ~400 screenings of a cDNA library by RACE.
The cDNA synthesized by this kit contains a high proportion of full-length cDNA because an "advanced type M-MLV Reverse Transcriptase" is used.
| Documents & Links for accura-expRACE KIT | |
| Datasheet | elp-epi6201-ex_accura-exprace-kit_datasheet.pdf |
| Vendor Page | accura-expRACE KIT at Cosmo Bio LTD |
| Documents & Links for accura-expRACE KIT | |
| Datasheet | elp-epi6201-ex_accura-exprace-kit_datasheet.pdf |
| Vendor Page | accura-expRACE KIT |