ABTS Antioxidant Capacity Assay Kit

BQCkit

Catalog No: BQC-KF01002-100

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Purpose of Usage

A solution of DMPD at an acidic pH, in the presence of a suitable oxidant solution, can form a stable and colored radical cation (DMPD•+) that shows maximum absorbance at 553 nm. Antioxidant compounds which are able to transfer a hydrogen atom to DMPD•+ quench the color and produce discoloration of the solution which is proportional to their amount. This reaction is rapid and the endpoint, which is stable, corresponds to a measure of the antioxidative efficiency.

This kit measures the antioxidant activity of compounds that are able to transfer hydrogen atoms.ABTS assay kit involves the direct production of the blue/green ABTS•+ chromophore, which has absorption maxima at 734 nm. After that, antioxidant compounds present in the sample reduce ABTS and produce a decoloration of the solution which is proportional to the antioxidant activity and the concentration of the antioxidant.
This reaction is rapid and the endpoint, which is stable, corresponds to a measure of the antioxidative efficiency.
BQC ABTS Assay Kit is recommended for the total antioxidant activity of solutions of pure substances, aqueous mixtures, and beverages.

Publications

• Narciso, Joan Oñate, and Laura Nyström. 2020. “Breathing New Life to Ancient Crops: Promoting the Ancient Philippine Grain ‘Kabog Millet’ as an Alternative to Rice.” Foods 9 (12): 1727. https://doi.org/10.3390/foods9121727 and a special procceding at Narciso, Joan Oñate, and Laura M Nyström. n.d. “Proceedings 2020, 4, x; Doi: FOR PEER REVIEW.” Accessed December 9, 2020. www.mdpi.com/journal/proceedings.
• Káňová, Kristýna, Lucie Petrásková, Helena Pelantová, Zuzana Rybková, Kateřina Malachová, Josef Cvačka, Vladimír Křen, and Kateřina Valentová. 2020. “Sulfated Metabolites of Luteolin, Myricetin, and Ampelopsin: Chemoenzymatic Preparation and Biophysical Properties.” Journal of Agricultural and Food Chemistry 68 (40): 11197–206. https://doi.org/10.1021/acs.jafc.0c03997
• Rajha, Hiba N, Roula M Abdel-massih, Richard G Maroun, Espérance Debs, and Nicolas Louka. 2020. “‘Intensification of Vaporization by Decompression to the Vacuum’ (IVDV), a Novel Technology Applied as a Pretreatment to Improve Polyphenols Extraction from Olive Leaves.” Food Chemistry, no. Ivdv: 128236. https://doi.org/10.1016/j.foodchem.2020.128236
• Biedermann, David, Veronika Moravcová, Kateřina Valentová, Marek Kuzma, Lucie Petrásková, Ivana Císařová, and Vladimír Křen. 2019. “Oxidation of Flavonolignan Silydianin to Unexpected Lactone-Acid Derivative.” Phytochemistry Letters 30 (April): 14–20. https://doi.org/10.1016/j.phytol.2019.01.006
• Rydlová, Bc Lenka. 2017. “ENZYMOVÉ MODIFIKACE BIOLOGICKY AKTIVNÍCH FLAVONOIDŮ Enzymatic Modifications of Bioactive Flavonoids.” UNIVERZITA KARLOVA V PRAZE.
• Biedermann, David, Veronika Moravcová, Kateřina Valentová, Marek Kuzma, Lucie Petrásková, Ivana Císařová, and Vladimír Křen. 2019. “Oxidation of Flavonolignan Silydianin to Unexpected Lactone-Acid Derivative.” Phytochemistry Letters 30 (January): 14–20. https://doi.org/10.1016/j.phytol.2019.01.006
• Rydlová, Lenka, Eva Tesařová, Kateřina Valentová, and Vladimír Křen. 2017. “Enzymatic Modifications of Bioactive Flavonoids Diplomová.” UNIVERZITA KARLOVA V PRAZE.
• Káňová, K., Petrásková, L., Pelantová, H., Rybková, Z., Malachová, K., Cvačka, J., … Valentová, K. (2020). Sulfated Metabolites of Luteolin, Myricetin, and Ampelopsin: Chemoenzymatic Preparation and Biophysical Properties. Journal of Agricultural and Food Chemistry, 68(40), 11197–11206. http://doi.org/10.1021/acs.jafc.0c03997
• Abi-Khattar, A.-M., Rajha, H. N., Abdel-Massih, R. M., Habchi, R., Maroun, R. G., Debs, E., & Louka, N. (2020). “Intensification of vaporization by decompression to the Vacuum” (IVDV), a novel technology applied as a pretreatment to improve polyphenols extraction from olive leaves
• Joan Oñate Narciso and Laura M. Nyström (2020) Alternative Grain Crops: Introducing the Kabog Millet from the Philippines as a Functional Food Ingredient †