Background
Stable three-dimensional culture (3D culture) can be performed easily without the need for neutralization or mixing with cell culture medium usually required to form atelocollagen gels. High-purity atelocollagen derived from bovine hide is premixed with DMEM, so it can be used immediately. Simply mix with the cells and warm it up. This product is especially recommended for those beginning the 3D culture without worrying about fibril formation during mixing the solutions or cell sedimentation during gel formation.
Features
- Ready-to-use atelocollagen solution for 3D culture.
- Premixed with DMEM, low glucose or high glucose.
- Easy operation: simply mix with the cells and warm to form a gel.
- Reduces cell sedimentation during gel formation achieves a stable 3D culture.
This product is manufactured in Japan and produced from raw materials obtained from livestock certified born and raised in Australia or New Zealand. Japan, Australia and New Zealand are all recognized by the World Health Organization as having negligible risk for Bovine Spongiform Encephalitis (BSE). Nevertheless, please consult with your country's Customs office to confirm importability.
| Documents & Links for 3D Ready Atelocollagen, DMEM, High Glucose | |
| Manual | 3D Ready Atelocollagen, DMEM, High Glucose Manual |
| Documents & Links for 3D Ready Atelocollagen, DMEM, High Glucose | |
| Manual | 3D Ready Atelocollagen, DMEM, High Glucose Manual |
| Citations for 3D Ready Atelocollagen, DMEM, High Glucose – 7 Found |
| Sagara, Atsunobu; Miura, Shotaro; Kobinata, Akinori; Naganawa, Risa; Yaginuma, Saki; Saito, Suguru; Saito, Rintaro; Kominato, Hidenori; Yumoto, Tetsuro; Sato, Fumiaki. COL8A1 enhances the invasion/metastasis in MDA-MB-231 cells via the induction of IL1B and MMP1 expression. Biochemical And Biophysical Research Communications. 2023;642( 36577251):145-153. PubMed - Comments: 3D culture |
| Iwahashi, Hiroyasu; Kawashima, Yoshihito; Masaki, Hitoshi. Decreased levels of endocytic collagen receptor Endo180 in dermal fibroblasts lead to decreased production of type I collagen and increased expression of matrix metalloproteinase-1. Photodermatology, Photoimmunology & Photomedicine. 2022;38(2):150-157. PubMed - Comments: 3D culture |
| Machihara, Kayo; Kageyama, Sou; Oki, Shoma; Makino, Hiroki; Sasaki, Masamichi; Iwahashi, Hiroyasu; Namba, Takushi. Lotus germ extract rejuvenates aging fibroblasts via restoration of disrupted proteostasis by the induction of autophagy. Aging. 2022;14(19):7662-7691. PubMed - Comments: 3D culture |
| Fujita, Mayumi; Imadome, Kaori; Somasundaram, Veena; Kawanishi, Miki; Karasawa, Kumiko; Wink, David A. Metabolic characterization of aggressive breast cancer cells exhibiting invasive phenotype: impact of non-cytotoxic doses of 2-DG on diminishing invasiveness. Bmc Cancer. 2020;20(1):929. PubMed - Comments: 3D culture |
| Miyazaki, Takuro; Taketomi, Yoshitaka; Higashi, Takayoshi; Ohtaki, Hirokazu; Takaki, Takashi; Ohnishi, Koji; Hosonuma, Masahiro; Kono, Nozomu; Akasu, Risako; Haraguchi, Shogo; Kim-Kaneyama, Joo-Ri; Otsu, Kinya; Arai, Hiroyuki; Murakami, Makoto; Miyazaki, Akira. Hypercholesterolemic Dysregulation of Calpain in Lymphatic Endothelial Cells Interferes With Regulatory T-Cell Stability and Trafficking. Arteriosclerosis, Thrombosis, And Vascular Biology. 2023;43(2):e66-e82. PubMed - Comments: Miscellaneous |
| Hanaki, Yusuke; Miyoshi, Sena; Sugiyama, Yasunori; Yanagita, Ryo C; Sato, Masashi. 12-O-Tetradecanoylphorbol 13-acetate promotes proliferation and epithelial-mesenchymal transition in HHUA cells cultured on collagen type I gel: A feasible model to find new therapies for endometrial diseases. Bioscience, Biotechnology, And Biochemistry. 2022;86(10):1417-1422. PubMed - Comments: Miscellaneous |
| Yamazaki, Shintaro; Ohka, Fumiharu; Hirano, Masaki; Shiraki, Yukihiro; Motomura, Kazuya; Tanahashi, Kuniaki; Tsujiuchi, Takashi; Motomura, Ayako; Aoki, Kosuke; Shinjo, Keiko; Murofushi, Yoshiteru; Kitano, Yotaro; Maeda, Sachi; Kato, Akira; Shimizu, Hiroyuki; Yamaguchi, Junya; Adilijiang, Alimu; Wakabayashi, Toshihiko; Saito, Ryuta; Enomoto, Atsushi; Kondo, Yutaka; Natsume, Atsushi. Newly established patient-derived organoid model of intracranial meningioma. Neuro-Oncology. 2021;23(11):1936-1948. PubMed - Comments: Miscellaneous |
