2D-Silver Stain Reagent II (for 10 gels)

Cosmo Bio LTD

Catalog No: DCB-423413

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The original silver staining method is cumbersome and takes a long-time to complete. 2D-Silver Stain Reagent II was developed to provide simple, fast and highly sensitive silver staining. It is applicable to the detection of proteins and nucleic acids subjected to polyacrylamide and SDS-polyacrylamide slab gel electrophoresis.

Detection of proteins and nucleic acids migration by polyacrylamide gel electrophoresis and silver staining are now being highlighted as one of the most sensitive staining methods. However, the original silver staining method is not necessarily easy to work and takes a long-time to complete. 2D-SILVER STAIN-II is an improved reagent kit developed to provide simple and fast staining.

Features

•Rapid staining results after electrophoresis
•High sensitivity
   ◦For proteins: 50 to 100 times more sensitivity than CBB staining
   ◦For nucleic acids: 50 to 100 times more sensitivity than EB staining
•Simple preparation and simple operation
•No use of heavy metals which need to be controlled by regulations
•Staining process can be stopped any time during development for desired results

Package size and Storage

•Sufficient to stain 10 slabs gels (140 mm x 140 mm x 1.0 mm)
•Store in a dark place at 2-10°C
•Shelf life is printed on the package

Citations for 2D-Silver Stain Reagent II (for 10 gels) – 14 Found

Fujimoto, Shigeyuki; Tsuji, Tokuji; Fujiwara, Takashi; Takeda, Taka-Aki; Merriman, Chengfeng; Fukunaka, Ayako; Nishito, Yukina; Fu, Dax; Hoch, Eitan; Sekler, Israel; Fukue, Kazuhisa; Miyamae, Yusaku; Masuda, Seiji; Nagao, Masaya; Kambe, Taiho. The PP-motif in luminal loop 2 of ZnT transporters plays a pivotal role in TNAP activation. The Biochemical Journal. 2016;473(17):2611-21. 

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Ishiguro, Sumie; Nishimori, Yuka; Yamada, Miho; Saito, Hiroko; Suzuki, Toshiya; Nakagawa, Tsuyoshi; Miyake, Hiroshi; Okada, Kiyotaka; Nakamura, Kenzo. The Arabidopsis FLAKY POLLEN1 gene encodes a 3-hydroxy-3-methylglutaryl-coenzyme A synthase required for development of tapetum-specific organelles and fertility of pollen grains. Plant & Cell Physiology. 2010;51(6):896-911. 

Okuda, Hiroaki; Tatsumi, Kouko; Morita, Shoko; Shibukawa, Yukinao; Korekane, Hiroaki; Horii-Hayashi, Noriko; Wada, Yoshinao; Taniguchi, Naoyuki; Wanaka, Akio. Chondroitin sulfate proteoglycan tenascin-R regulates glutamate uptake by adult brain astrocytes. The Journal Of Biological Chemistry. 2014;289(5):2620-31. 

Tanaka, Yuka; Yamada, Shiori; Connop, Samantha L; Hashii, Noritaka; Sawada, Hitoshi; Shih, Yu; Nishida, Hiroki. Vitelline membrane proteins promote left-sided nodal expression after neurula rotation in the ascidian, Halocynthia roretzi. Developmental Biology. 2019;449(1):52-61. 

Appiah-Kwarteng, Cornelia; Saito, Taizo; Toda, Natsuki; Kitoh, Katsuya; Nishikawa, Yoshibumi; Adenyo, Christopher; Kayang, Boniface; Owusu, Ebenezer Oduro; Ohya, Kenji; Inoue-Murayama, Miho; Kawahara, Fumiya; Nagamune, Kisaburo; Takashima, Yasuhiro. Native SAG1 in Toxoplasma gondii lysates is superior to recombinant SAG1 for serodiagnosis of T. gondii infections in chickens. Parasitology International. 2019;69( 30630114):114-120. 

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