Frequently Asked Questions

Adipose tissue related

■ Visceral fat cells

【2-01】 Can frozen and thawed cells be refrozen?

Avoid refreezing cells once thawed. We recommend half size ( 1.5x10 ^{6 cells x 1 part number: VACH1C).}

【2-02】 What is the expiration date of the visceral fat cell culture kit?

The shelf life of frozen cells is 1 year when frozen at -80°C.
The culture medium can be frozen at -20°C for 6 months and liquid nitrogen for 1 year.

【2-03】 Is passage possible?

We do not recommend subculturing because the rate of adipocyte differentiation is extremely low.

【2-04】 Is it possible to prepare mouse-derived cells?

We are considering commercialization.

【2-05】 Is 3D culture possible?

We have confirmed that culture in collagen gel is possible.
This can extend the number of days that can be cultured after maturation, but it is necessary to consider the amount of collagen in advance because the collagen dissolves.

■ Subcutaneous white fat cells

【2-06】 Is passage possible?

We have confirmed that neonatal products (WAT01, 02, 03) can only be used once.

■ Brown fat cells

【2-07】 Is passage possible?

We have confirmed that it can be passaged only once.

【2-08】 When measuring the expression of UCP1, how long should it be after administration?

When looking at mRNA expression, it has been confirmed that it rises sharply around 6 hours.