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Why To Use Ubiquigent Reagents

? Fully annotated protein sequence

? Gradient SDS-PAGE purity analysis

? Tandem mass-spectrometric confirmation of identity

? Activity determination in application-specific assays

? Background literature synopsis provided for all products

? Standard formulations and fixed concentrations

? Online datasheets

? Expert technical support
Ubiquigent Application Note

Validation and Experimental Utility of the E2scan™ Kit version 2

"Linking Ubiquitin Research to Drug Discovery"

Ubiquigent - Kinases

Like ubiquitylation, phosphorylation is a reversible post-translational modification: Phosphorylation reactions involve the transfer of a phosphate group from a high-energy donor molecule, such as ATP, to a substrate protein or lipid, catalyzed by enzymes called kinases and is a process central to intracellular signaling. Abnormal protein phosphorylation is a cause or consequence of disease, including cancers, diabetes and inflammatory disease, while defects in genes that encode protein kinases and phosphatases underlie a number of inherited disorders. Around 500 protein kinases are encoded in the human genome and they account for around 25% of the molecular targets currently being pursued by drug discovery companies. It is becoming increasingly apparent that there is significant cross-talk between these two key intracellular systems; phosphorylation and ubiquitylation. For example TBK1 is an IKK-related kinase, which plays several important roles in the innate immune system. In the MyD88-dependent signaling pathway its activation requires the E3 ubiquitin ligase TRAF6 and the polyubiquitin-binding protein NEMO. TBK1 also plays an essential role in the production of type1 interferons that are produced in response to viral double-stranded RNA. This is triggered by the TBK1-catalysed activation of the transcription factor IRF3 and the E3 ubiquitin ligase Pellino 1.

Catalog Number	Product Name	Size	Datasheet
UBI-66-0001-050	BRSK2 residues (2-674) [6His-tagged]	50 ug	Datasheet
UBI-66-0004-050	MARK 3 [6His-tagged]	50 ug	Datasheet
UBI-66-0005-050	MARK 4 [6His-tagged]	50 ug	Datasheet
UBI-66-0007-050	TAK1_TAB1 complex [6His-tagged]	50 ug	Datasheet
UBI-66-0014-050	PKA [GST-tagged]	50 ug	Datasheet
UBI-66-0016-050	TBK1 [GST-tagged]	50 ug	Datasheet
UBI-66-0017-050	PKB Alpha (S473D) [6His-tagged]	50 ug	Datasheet
UBI-66-0018-050	PKB Alpha [GST-tagged]	50 ug	Datasheet
UBI-66-0019-050	CHK2 [GST-tagged]	50 ug	Datasheet
UBI-66-0020-050	SGK1 (S422D) [6His-tagged]	50 ug	Datasheet
UBI-66-0021-050	SGK3 [GST-tagged]	50 ug	Datasheet
UBI-66-0022-050	B-Raf [GST-tagged]	50 ug	Datasheet
UBI-66-0023-050	B-Raf (V600E) [GST-tagged]	50 ug	Datasheet
UBI-66-0024-050	C-Raf [GST-tagged]	50 ug	Datasheet
UBI-66-0026-050	MAPK3 [untagged]	50 ug	Datasheet
UBI-66-0027-050	MAPK7 [6His-tagged]	50 ug	Datasheet
UBI-66-0028-050	MAPK8 [6His-tagged]	50 ug	Datasheet
UBI-66-0029-050	MAPK9 [6His-tagged]	50 ug	Datasheet
UBI-66-0030-050	MAPK10 [GST-tagged]	50 ug	Datasheet
UBI-66-0031-050	MAPK11 [GST-tagged]	50 ug	Datasheet
UBI-66-0032-050	MAPK12 [GST-tagged]	50 ug	Datasheet
UBI-66-0034-050	MAPK14 [GST-tagged]	50 ug	Datasheet
UBI-66-0035-050	MAPK14 (T106M) [GST-tagged]	50 ug	Datasheet
UBI-66-0036-050	MAPK15 [6His-tagged]	50 ug	Datasheet
UBI-66-0038-050	IKK Epsilon [GST-tagged]	50 ug	Datasheet
UBI-66-0039-050	p70 S6 kinase (T412E) [6His-tagged]	50 ug	Datasheet
UBI-66-0040-050	AMPK alpha 1 (T172D) [GST-tagged]	50 ug	Datasheet
UBI-66-0041-050	AMPKA1/AMPKB2/AMPKG1 [6His-tagged]	50 ug	Datasheet
UBI-66-0042-050	AMPKA2/AMPKB2/AMPKG1 [6His-tagged]	50 ug	Datasheet
UBI-66-0043-050	PINK1 [MBP-tagged]	50 ug	Datasheet
UBI-66-0044-050	PINK1 (D359A) [MBP-tagged]	50 ug	Datasheet