E1 activating enzymes initiate the activation and conjugation of ubiquitin and ubiquitin-like modifiers (UBLs) in a cascade of events that modifies substrate proteins resulting in proteasomal or lysosomal degradation, changes in intracellular localization, activity or other signalling events.
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Ubiquitin Chains - Why To Use Ubiquigent Reagents
● Fully annotated protein sequence
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"Linking Ubiquitin Research to Drug Discovery"
Ubiquigent - E1 Activating Enzymes
To date eight E1 enzymes have been identified, two characterized from the human genome comprise three domains; an adenylation domain which binds ATP and ubiquitin, a catalytic cysteine domain (CCD) which binds activated ubiquitin, and a C-terminal ubiquitin-fold domain (Ufd) which recruits specific E2 conjugating enzymes. E1 enzymes utilise ATP to activate the terminal glycine residue of ubiquitin/UBL generating a covalent thioester linkage between ‘activated’ ubiquitin/UBL and the E1 enzyme itself. Ubiquitin/UBL is then transferred to the sulphydryl group of the active-site cysteine on an E2 conjugating enzyme in a transthiolation reaction.
| Catalog Number | Product Name | Size | Datasheet |
| UBI-61-0001-010 | UBE1 [6His-tagged] | 10 ug | Datasheet |
| UBI-61-0001-010 | UBE1 [6His-tagged] | 50 ug | Datasheet |
| UBI-61-0002-010 | UBA6 [6His-tagged] | 10 ug | Datasheet |
| UBI-61-0002-010 | UBA6 [6His-tagged] | 50 ug | Datasheet |
| UBI-61-0005-010 | SAE1 [GST-tagged] / SAE2 [6His-tagged] | 10 ug | Datasheet |
| UBI-61-0005-010 | SAE1 [GST-tagged] / SAE2 [6His-tagged] | 50 ug | Datasheet |
| UBI-61-0006-010 | APP-BP1 [untagged] / UBA3 [untagged] | 10 ug | Datasheet |
| UBI-61-0006-010 | APP-BP1 [untagged] / UBA3 [untagged] | 50 ug | Datasheet |
| UBI-61-0007-010 | UBA7 [6His-tagged] | 10 ug | Datasheet |
| UBI-61-0007-010 | UBA7 [6His-tagged] | 50 ug | Datasheet |
| UBI-61-0008-010 | ATG7 [6His-tagged] | 10 ug | Datasheet |
| UBI-61-0008-010 | ATG7 [6His-tagged] | 50 ug | Datasheet |