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Ubiquitin Chains - Why To Use Ubiquigent Reagents
● Fully annotated protein sequence
● Gradient SDS-PAGE purity analysis
● Tandem mass-spectrometric confirmation of identity
● Activity determination in application-specific assays
● Background literature synopsis provided for all products
● Standard formulations and fixed concentrations
● Online datasheets
● Expert technical support - Ubiquigent Application Note
Validation and Experimental Utility of the E2scan™ Kit version 2
"Linking Ubiquitin Research to Drug Discovery"
Ubiquigent - E1 Activating Enzymes
E1 activating enzymes initiate the activation and conjugation of ubiquitin and ubiquitin-like modifiers (UBLs) in a cascade of events that modifies substrate proteins resulting in proteasomal or lysosomal degradation, changes in intracellular localization, activity or other signalling events.
To date eight E1 enzymes have been identified, two characterized from the human genome comprise three domains; an adenylation domain which binds ATP and ubiquitin, a catalytic cysteine domain (CCD) which binds activated ubiquitin, and a C-terminal ubiquitin-fold domain (Ufd) which recruits specific E2 conjugating enzymes. E1 enzymes utilise ATP to activate the terminal glycine residue of ubiquitin/UBL generating a covalent thioester linkage between ‘activated’ ubiquitin/UBL and the E1 enzyme itself. Ubiquitin/UBL is then transferred to the sulphydryl group of the active-site cysteine on an E2 conjugating enzyme in a transthiolation reaction.
| Catalog Number | Product Name | Size | Datasheet |
| UBI-61-0001-010 | UBE1 [6His-tagged] | 10 ug | Datasheet |
| UBI-61-0001-010 | UBE1 [6His-tagged] | 50 ug | Datasheet |
| UBI-61-0002-010 | UBA6 [6His-tagged] | 10 ug | Datasheet |
| UBI-61-0002-010 | UBA6 [6His-tagged] | 50 ug | Datasheet |
| UBI-61-0005-010 | SAE1 [GST-tagged] / SAE2 [6His-tagged] | 10 ug | Datasheet |
| UBI-61-0005-010 | SAE1 [GST-tagged] / SAE2 [6His-tagged] | 50 ug | Datasheet |
| UBI-61-0006-010 | APP-BP1 [untagged] / UBA3 [untagged] | 10 ug | Datasheet |
| UBI-61-0006-010 | APP-BP1 [untagged] / UBA3 [untagged] | 50 ug | Datasheet |
| UBI-61-0007-010 | UBA7 [6His-tagged] | 10 ug | Datasheet |
| UBI-61-0007-010 | UBA7 [6His-tagged] | 50 ug | Datasheet |
| UBI-61-0008-010 | ATG7 [6His-tagged] | 10 ug | Datasheet |
| UBI-61-0008-010 | ATG7 [6His-tagged] | 50 ug | Datasheet |