HIGH DENSITY SINGLE CELL ARRAY DEVICE

Not your typical 370,000 and 90,000 microwell plates!
SIEVEWELL™ is a new, innovative microfluidics platform for single cell capture, culture, imaging and analysis.

Ordering Information

Product name (click for order info)

Cat no (click for user guide)

Size

The SIEVEWELL™ Slide: A new microfluidic device for ultra-high density single cell capture, culture, processing and imaging

TOK-SWS-2001-5

TOK-SWS-5001-5

370.000 wells per slide, well size = 20 microns across each (5 pack)

90,000 wells per slide, well size = 50 microns across
(5 pack)

About SIEVEWELL™

At the heart of Sievewell is an ultra-high density, optically transparent membrane array of cell sized, cell culture-friendly wells precision manufactured by photolithography. Each well contains 2 pores through which fluid can pass. Due to SIEVEWELL's unique design, suction applied through Sievewell’s fluid aspiration ports (with a hand pipette) creates a downward flow. This innovative design enables:

Single cell per well capture rates significantly higher than by gravity alone
At-will fluid exchange with downward flow working to keep cells in place. Ideal for cell washing, staining, and other treatment to cells.
Simple optical observation, imaging and evaluation.

For much more product and application information, please download the Sievewell 8-page Product Brochure (sidebar this page), browse the image gallery below, and visit SIEVEWELL.COM.

The following list indexes the page content at SIEVEWELL.COM, the manufacturer's site (external links).

Home

• Introductory image slider with links to other pages and videos on SIEVWELL.COM.
• Don't Miss Your Rare Cells (with introductory video); Arraying Cells at High Density; On-chip Staining Without Cell Loss; No Pump Needed. Just Pipette; Single Cell Culture.

Product

• Product Specifications; 8-page brochure download; SIEVEWELL FAQ

How it works

• Introductory Video (same as on Home page)

Features

• Membrane Film With Microwells; No Pump Required. Just Pipette; On-chip Staining Without Cell Loss; Single cell culture

Applications

• Single cell culture
time lapse of K562 cells cultured on 50 micron Sievewell slide.

• Cell-Cell Interaction Assays
Monitor NK cell cytotoxicity

• Calcium Flux Assay in Suspension Cells
SIEVEWELL™ vs standard microplate using Fluo4-AM treated K562 cells followed by ionomycin addition.

• Culture of Cardiomyocytes and Calcium Imaging
Cell culture and imaging of IPS cell-derived cardiomyocytes expressing a calcium sensor. Video shows culture of cardiomyocytes and calcium cycling.

• On-chip Neural differentiation of PC12 cells
Rat pheochromocytoma PC12 cells stained with anti-tubulin ß3 and DAPI after culture with NGF

• Detection of circulating tumor cells from CDX/PDX-tumor bearing mouse
Blood cells from mouse xenografted with fluorescent-labeled tumor loaded on Sievewell and lysed.

• Detection of Neutrophil extracellular traps (NETs)
Stimulation of human PBMC by PMA and staining and visualization of NETs

• Real-time imaging to quantify NETosis with SIEVEWELL

• Mouse blood separation
Separation of lymphocytes from small volume of mouse blood

• Tissue dissociation on chip
Direct dissociation of mouse tissue to obtain rare cell population

• On chip CTC staining, detection
Staining and detection of human PBMC spiked with MCF

• Imaging with Confocal Microscopy

Library

One-page flyer
References Page
Procedures Page:

• General Use Notes Videos (focus on pipette use for fluid control)
• Step 1 Videos - priming the slide
• Step 2 Videos - procedure for cell loading
• Step 3 Videos - procedure for cell staining