Reproductive Engineering Techniques in Mice by Naomi Nakagata (3rd Edition)

KYUDO/CARD Products for Mouse Reproductive Engineering

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Currently in a its 3rd Edition, a limited number of printed books are available from Cosmo Bio USA in English, French and Spanish. Please contact us for details.

REPRODUCTIVE ENGINEERING TECHNIQUES IN MICE
Copyright©2015 Naomi Nakagata
ALL RIGHTS RESERVED. PRINTED IN JAPAN. This book is not to be sold.
No part of this publication may be reproduced, stored in a retrieval
system or transmitted in any form or by any means, electronic,
mechanical, photocopying, recording or otherwise, without prior
permission of the copyright holder.

REPRODUCTIVE ENGINEERING TECHNIQUES IN MICE (3rd Edition)

	Full Version	Part 1	Part 2
English	Download	Download	Download
French	Download	Download	Download
Spanish	Download	Download	Download

Download by Chapter

Chapter	English	French	Spanish
Chapter 1 - In Vitro Fetilization	Download	Download	Download
Chapter 2 - Transportation of Sperm	Download	Download	Download
Chapter 3 - Cryopreservaton of Sperm	Download	Download	Download
Chapter 4 - Preparation of Oocyte and Embryo	Download	Download	Download
Chapter 5 - Transportation of Oocyte and Embryo	Download	Download	Download
Chapter 6 - Cryopreservation of Oocyte and Embryo	Download	Download	Download
Chapter 7 - Other Techniques	Download	Download	Download
Chapter 8 - Media	Download	Download	Download

Table of Contents

Chapter 1: In Vitro Fertilization (IVF) (pdf)

• 1-1 Preparing and Assembling Pipettes for Embryo Handling p4
  
  Kyudo products used: Embryo Manipulation Set
  
  
• 1-2 In Vitro Fertilization (IVF) p6
  Videos: | Removing the Cauada Epididymides | Releasing Seprmatozoa | Removing the Oviduct | Introducing Cumulus-Oocyte Complex (COCs) into a drop of CARD MEDIUM |
  Kyudo products used: CARD Fertiup® Preincubation Media, CARD MEDIUM®, CARD mHTF
  
  
• 1-3 In Vitro Fertilization (IVF) using Ultra-Superovulation Reagent p12
  Kyudo products used: HyperOva®

 

Chapter 2: Transportation of Sperm (pdf)

• 2-1 Collection and Transport at Cold Temperature of Cauda Epididymis p14
  Videos: | Removing one Cauda Epididymis from an Anesthetized Male | Removing the Sample
  Kyudo products used: CARD MEDIUM®, CARD Cold Transport Kit
  
  
• 2-2 In Vitro Fertilization using Epididymal Sperm Transported at Cold Temperature p18
  
• Videos: | Removing the Sample |
  Kyudo products used: CARD MEDIUM®, CARD mHTF,

 

Chapter 3: Cryopreservation of Sperm (pdf)

• 3-1 Cryopreservation of Mouse Spermatozoa p20
  Videos: | Cutting the Epididymis and Preparing Sperm Suspension | Freezing the Straws |
  Kyudo products used: CARD Fertiup® Cryoprotectant, CARD mHTF, Straw Connector, Sperm Straws, Triangular Cassette, Freezing Canister
   
• 3-2 In Vitro Fertilization using Cryopreserved Spermatozoa p26
  Videos: | Thawing of Mouse Spermatazoa | Transfer of the Thawed Sperm Suspension | Aspiration of Preincubated Sperm Suspension and Insemination of Oocytes |
  KYD products used: CARD Fertiup® Preincubation Media, CARD Medium®, CARD mHTF, Straw Connector, Sperm Straw
  
  
• 3-3 Rescue In Vitro Fertilization Method for Legacy Stock of Cryopreserved Spermatozoa p32
  Kyudo products used: CARD Fertiup® Preincubation Media, CARD Medum®, CARD mHTF, Sperm Straw

 

Chapter 4: Preparation of Oocyte & Embryo (pdf)

• 4-1 Preparation of Laser-micro-dissected Oocytes p36
  Videos: | Dissection of Zona Pellucida using a Laser |
  Kyudo products used: CARD MEDIUM®, CARD mHTF
  
  
• 4-2 Partial Zona Dissection (PZD) p39
  Videos: | PZD |
  Kyudo products used: CARD Medium, CARD Sucrose, mHTF
  
  
• 4-3 Collecting 2-Cell Stage Embryos p42
  Videos: | Flushing |
  Kyudo products used: CARD KSOM, 

 

Chapter 5: Transportation of Oocyte & Embryo (pdf)

• 5-1 Transport of 2-Cell Embryos at Cold Temperature p46
  Videos: | Removing the Sample |
  Kyudo products used: CARD KSOM, Embryo Manipulation Set, Cold Transport Kit
  
  
• 5-2 Transportation of Mouse Oviducts Containing 2-Cell Embryos at Low Temperature p52
  Kyudo products used: CARD Sucrose, CARD KSOM

 

Chapter 6: Cryopreservation of Oocyte & Embryo (pdf)

• 6-1 Simple Vitrification of Mouse Embryos p54
  Videos: | Vitrifying Embryos | Recovering Vitrified Embryos | Pipetting to Recover Vitrified Embryos |
  Kyudo products used: CARD DMSO, CARD DAP213, CARD Sucrose, CARD KSOM
  
  
• 6-2 Simple Vitrification of Mouse Oocytes p59
  Kyudo products used: CARD Fertiup® Cryoprotectant, CARD mHTF
  
  
• 6-3 Vitrification and Transplantation of Mouse Ovaries p62
  Videos: | Transplantation to Mouse Ovary |

Chapter 7: Other Techniques (pdf)

• 7-1 Vasectomy for the Creation of Sterile Males p64
  
  
• 7-2 Embryo Transfer into the Oviduct 66
  Videos: | Transferring Embryos to the Ovaduct |
  
  
• 7-3 Embryo Transfer into the Uterus p72
  Videos: | Embryo Transfer into the Uterus | Performing the Operation  |  
  Kyudo products used: CARD KSOM

• 7.4 Caesarean Section and Fostering p76
• Videos: | From the Removal of the Uteruses through to the first breath of the Pups | Fostering |
  

REPRODUCTIVE ENGINEERING TECHNIQUES IN MICE (3rd Edition) Table of Contents>/h4>

Chapter 8: Media (pdf)

• 8-1 Storage of Media and Solutions in Ampoules Under Nitrogen Gas p78
  
  
• 8-2 Table of Media Composition p79

 

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