- this page: PUREfrex® Products Dashboard
- PUREfrex® Ordering Information Table
Announcements
Formulation Changes (Jan 2022)
- PUREfrex 2.0 mini
- (entry model)
- PUREfrex 2.0
- (for protein synthesis)
- PUREfrex 2.1
(for proteins with disulfide bonds) - PUREfrex 1.0
(for basic research and mechanism studies) - PUREfrex 1.0 Custom
(formulated to your specs) - Reaction Supplements
- (EF-P, DnaK, GroE, DsbC Set, PDI Set)
PUREfrex Product Manual and Package Inserts
- PUREfrex 2.0/2.1 Kit Technical Manual
- 2.0/2.1 full version (34 pages)
- Package Inserts
- PUREfrex Kits
(reagent sets for Y ml rxn mix x number of reagent sets) - 2.0 mini - 100ul x 1 set
2.0 - 0.25 mL x 1 set
2.0 - 0.25 mL x 5 sets to be discontinued
2.0 - 2 mL x 1 set
2.0 - 2 mL x 5 sets
2.0 - 2 ml x 25 sets new size
2.1 - 250uL x 1 set
2.1 - 250uL x 5 sets to be discontinued
2.1 - 2mL x 1 set
2.1 - 2mL x 5 sets
2.1 - 2 ml x 25 sets new size
1.0 - 250uL x 1 set
1.0 - 250uL x 5 sets to be discontinued
1.0 - 2mL x 1 set
1.0 - 2mL x 5 sets
1.0 - 2 ml x 25 sets new size - PUREfrex Reaction Supplements
(reagent sets for Y ml rxn mix x number of reagent sets)
DnaK Mix - 500 uL x 1 set
DnaK Mix - 2 mL x 5 sets
DnaK Mix - 2 ml x 25 sets new size
GroE Mix - 500uL x 1 set
GroE Mix - 2mL x 5 sets
GroE Mix - 2 ml x 25 sets new size
DsbC Set - 500uL x 1 set
DsbC Set - 2mL x 5 sets
DsbC Set - 2 ml x 25 sets new size
PDI Set - 500uL x 1 set
PDI Set - 2 mL x 5 sets
PDI Set - 2 ml x 25 sets new size
EF-P - 500uL x 1 set
EF-P - 2mL x 5 sets
EF-P - 2 ml x 25 sets new size
- PUREfrex Kits
- Brochure
Download
Key Technical Notes
- ♦ PUREfrex Template Preparation
- ♦ Six Tips on PUREfrex DNA Template Design
- ♦ Synthesis of Protein Toxin (Gelonin) using DnaK Mix
- ♦ Sequence of DHFR DNA
- ♦ Influence of reducing agents on the redox state
- ♦ Improvement of specific activity by methylation of release factors (RF1, RF2).
- ♦ Improvement of the translation efficiency of consecutive proline residue-containing proteins by EF-P.
- ♦ Synthesis of proteins containing disulfide bonds with PDI and DsbC.
- ♦ Synthesis of functionally active proteins having disulfide bonds.
PUREfrex FAQ
PUREfrex in the Literature
- Citation of the Month: Jensen, et. al., Cell free protein synthesis versus yeast expression - A comparison using insulin as a model protein.
- Literature Citations
- Annotated Bibliography (pdf)
Manufacturer's Site Links
Safety Data Sheets
- 2.0 SDS USA EU
- 2.1 SDS USA EU
- 1.0 SDS USA EU
- DnaK Mix USA EU
- GroE Mix USA EU
- DscC Set USA EU
- PDI Set USA EU
- EF-P USA EU
Posters
- General Protein Synthesis
- Improvement of translational efficiency by N-terminal codon optimization in the reconstituted cell-free protein synthesis system [2016]
- Improvement of the translation efficiency of proline residue-containing proteins using the reconstituted cell-free protein synthesis system (PUREfrex) [2018] VIEW
- Investigation on how to synthesize active proteins by using a reconstituted cell-free protein synthesis system (PUREfrex) [2020]
- Disulfide Bonding
- Synthesis of proteins containing disulfide bonds using a reconstituted cell-free protein synthesis system (PUREfrex). [2019]
- Synthesis of proteins containing disulfide bonds using PUREfrex® with human protein disulfide isomerase [2018] VIEW
- Synthesis of functionally active proteins containing disulfide bonds using the new PURE system (PUREfrex) [2011]
- Synthesis of Immunoglobulins and Biologics
- Unique Cell-Free Protein Synthesis System, PUREfrex -Useful Platform for Protein Expression in the Development of Biologics- [2018] VIEW
- Preparation of labeled cyclic peptides with PUREfrex [2017]
- Development of a method for the synthesis of aglycosylated full-length IgG using PUREfrex [2017]
- Efficient in vitro expression of aglycosylated full-length IgG using a reconstituted cell-free protein synthesis system, PUREfrex 2.0 [2016]
- In vitro production of Fab, Toxin, and Immunotoxin using PUREfrex®2.0 with increased productivity [2015]
- Synthesis of functionally active proteins containing disulfide bonds using the new PURE system (PUREfrex) [2011]
PUREfrex® custom
Overview
PUREfrex® is an ultra-cool, ultra-smart, and ultra-useful in vitro coupled transcription/translation system based on E.coli translation machinery but completely different from S30 translation extracts. PUREfrex® is NOT AN EXTRACT. PUREfrex® is a RECONSTITUTED SYSTEM comprising highly purified E. coli ribosomes, highly purified recombinant proteins, and other highly purified factors required for transcription, translation, aminoacylation, and energy regeneration. As such, PUREfrex kits provide previously unthinkable reaction controllability compared to cell extract-based systems and a well defined mix of molecular components from which to purify synthesized protein.
Which PUREfrex Kit Is Right For Me?
PUREfrex® coupled transcription/translation system kits are available in four versions.
| PUREfrex® 2.0 mini | PUREfrex 2.0 mini is an entry model designed for those who are trying protein synthesis for the first time with a cell-free system. |
| PUREfrex® 2.0 | Choose 2.0 when protein yield is you primary goal and disulfide bonding is not relevant. |
| PUREfrex® 2.1 | 2.1 kits are based on 2.0 reaction components but are not premixed with reducing agent. 2.1 kits include separate vials of cysteine, DTT, and reduced glutathione allowing control of the redox environment. Choose 2.1 kits for primarily for target proteins with disulfide bonds. |
| PUREfrex® 1.0 | 1.0 kits produce less protein than 2.X kits, but the complete contents of 1.0 kits is disclosed. Chose 1.0 when complete information on PUREfrex reaction components takes precedence over protein yield. |
| PUREfrex® 1.0 Custom | Gene Fronteir is happy to provide custom reaction mixtures whenever feasible. Custom PUREfrex kits are based on 1.0 reaction components. Please contact us with your request. |
Please Note: All protein components in all PUREfrex versions are tag free allowing the synthesis of protein with any desired tag.
About PUREfrex Custom Kits
With its high reaction component controllability, the PUREfrex reconstituted cell-free protein synthesis system enables applications for biological research and drug discovery that are problematic, inefficient, or impossible using extract translation systems. These include, to name a few, insertion of unnatural amino acids, ribosome/virus/mRNA in vitro display, and mechanistic analysis of prokaryotic translation.
PUREfrex custom kits are your convenient route to applications and experiments limited only by your imagination. Tell us what you need. If technically feasible, Gene Frontier will do all they can to meet your specifications and timelines.
Most PUREfrex custom kits are built from PUREfrex 1.0 reaction components, which are fully disclosed as described below. But please don't hesitate to contact us with any request.
PUREfrex 1.0 Reaction Components |
||
| Solution 1 (Buffers, Salts, Substrates | Solution II (proteins) | Solution III |
| Hepes-KOH pH 7.6 |
20 Aminoacyl-tRNA synthetases (ARS) |
Ribosomes |
| Potassium glutamate |
Methionyl-tRNA transformylase (MTF) |
|
| Magnesium acetate |
3 Initiation factors (IF1, IF2, IF3) |
|
| Spermidine |
3 Elongation factors (EF-Tu, EF-Ts, EF-G) |
|
| Dithiothreitol |
3 Release factors (RF1, RF2, RF3) |
|
| 10-Formyl-tetrahydrofolate |
Ribosome recycling factor (RRF) |
|
| 4 NTPs |
T7 RNA polymerase |
|
| 20 Amino acids |
Nucleoside diphosphate kinase (NDK) |
|
| Creatine phoshate |
Adenylate kinase (ADK, myokinase) |
|
| tRNA mixture |
Ceatine kinase (CK) |
|
|
Pyrophosphatase (PPiase) |
||
Custom Kit Examples
- (-) RF1: For synthesis of a protein containing a noncanonical amino acid by amber suppression as below.
- (-) T7 RNA polymerase: For use of other RNA polymerase such as E.coli RNA polymerase.
- (-) Specific amino acid(s) and/oraminoacyl-tRNA synthetase: For synthesis of a protein containing noncanonical amino acid or less than 20 amino acids.
PUREfrex Custom Kit Ordering Information
- Agreement
- Custom kits are sold under a usage rights agreement with the Gene Frontier (“GFC”). Usage rights are limited.
- Agreement terms (abridged)
- Materials are for internal research use only, and only for non-clinical use.
Material is for non-commercial use. Transfer of materials to any third party is prohibited.
Material is not to be modified beyond the Purpose of Use stated in the Agreement.
Customer shall promptly notify GFC if any new invention is made through the use of the Material or its components.
Any new invention relating to use of the Materials belongs to GFC.
Ownership of any products themselves synthesized through the use of the Materials belong to Customer.
Industrial use of the Materials for in vitro display purposes is prohibited without a separate “License Agreement.” - Agreement Procedure
- Request a custom use agreement by email stating purpose of use from Cosmo Bio USA (info@cosmobiousa.com).
Review the Agreement.
Request feasibility of your custom design including quantity, packaging, timing, etc.
Return the Agreement signed by an authorized signer.
Receive a catalog number for your request and pricing from Cosmo Bio USA.
Place order using the received catalog number.. - Request for Quotation
- Prices are set by reaction volume.
Sold in 1mL reaction volume increments. Minimum order is 1 mL.
Request price quotes from Cosmo Bio USA (info@cosmobiousa.com)
PUREfrex Ordering Information
| Product Name (click for price and order info) | Catalog Number | Total Reaction Volume | Notes |
|---|---|---|---|
| Entry Model Kits | |||
| PUREfrex® 2.0 mini | GFK-PF201-0.1-EX | 100 μL | New item ! |
| PUREfrex® 2.0 mini is an entry model designed for those who are trying protein synthesis for the first time with a cell-free system.Product Information Page：PUREfrex® 2.0 mini | |||
| Kits | |||
| PUREfrex® 2.0 | GFK-PF201-0.25-EX | 250 ul | |
| GFK-PF201-0.25-5-EX | 5 x 250 ul (packaged as 5 x 250 ul) | Sales to be discontinued | |
| GFK-PF201-2ML-EX | 1 x 2 ml | ||
| GFK-PF201-10ML-EX | 5 x 2 ml | Note: cat. no. change from GFK-PF201-10-EX | |
| GFK-PF201-50ML-EX | 25 x 2 ml | New size ! | |
| Current generation kit for protein synthesis. Contains DTT as a reducing agent. Product Information Page：PUREfrex® 2.0 | |||
| PUREfrex® 2.1 | GFK-PF213-0.25-EX | 250 ul | |
| GFK-PF213-0.25-5-EX | 5 x 250 ul (packaged as 5 x 250 ul) | Sales to be discontinued | |
| GFK-PF213-2ML-EX | 1 x 2 ml | New size! | |
| GFK-PF213-10ML-EX | 5 x 2 ml | Note: cat. no. change from GFK-PF213-10-EX | |
| GFK-PF213-50ML-EX | 25 x 2 ml | New size ! | |
| Current generation kit for proteins with disulfide bonds. Formulated for user control of redox environment. Product Information Page：PUREfrex® 2.1 | |||
| PUREfrex® 1.0 | GFK-PF001-0.25-EX | 250 ul | |
| GFK-PF001-0.25-5-EX | 1250 ul (packaged as 5 x 250 ul) | Sales to be discontinued | |
| GFK-PF001-2ML-EX | 1 x 2 ml | New size! | |
| GFK-PF001-10ML-EX | 5 x 2 ml | Note: cat. no. change from GFK-PF001-10-EX | |
| GFK-PF001-50ML-EX | 25 x 2 ml | New size ! | |
| Prior generation kit for protein science research and synthetic biology. Components are fully disclosed. Product Information Page：PUREfrex® 1.0 | |||
| Custom formulated kits are based on 1.0 components. Please inquire about PUREfrex® custom. | |||
| PUREfrex Reaction Supplements | |||
| DnaK Mix | GFK-PF003-0.5-EX | for 500 ul of reaction mix | |
| GFK-PF003-10ML-EX | 5 x 2 ml | Note: cat. no. change from GFK-PF003-10-EX | |
| GFK-PF003-50ML-EX | 25 x 2 ml | New size ! | |
| GroE Mix | GFK-PF004-0.5-EX | for 500 ul of reaction mix | |
| PF004-10ML-EX | 5 x 2 ml | Note: cat. no. change from GFK-PF004-10-EX | |
| PF004-50ML-EX | 25 x 2 ml | New size ! | |
| Supplements for synthesis of aggregate-prone proteins. Product Information Page：PUREFrex Reaction Supplements | |||
| DsbC Set | GFK-PF005-0.5-EX | for 500 ul of reaction mix | |
| PF005-10ML-EX | 5 x 2 ml | Note: cat. no. change from GFK-PF005-10-EX | |
| PF005-50ML-EX | 25 x 2 ml | New size ! | |
| PDI Set | GFK-PF006-0.5-EX | for 500 ul of reaction mix | |
| PF006-10ML-EX | 5 x 2 ml | Note: cat. no. change from GFK-PF006-10-EX | |
| PF006-50ML-EX | 25 x 2 ml | New size ! | |
| Supplements for synthesis of proteins containing disufide bonds.. Product Information Page：PUREFrex Reaction Supplements | |||
| EF-P | GFK-PFS052-0.5-EX | for 500 ul of reaction mix | |
| PFS052-10ML-EX | 5 x 2 ml | Note: cat. no. change from GFK-PFS052-10-EX | |
| PFS052-50ML-EX | 25 x 2 ml | New size ! | |
| Supplement for use when consecutive prolines are in target protein. Product Information Page：PUREFrex Reaction Supplements | |||
Notes
PUREfrex® is developed for in vitro research only. PUREfrex® should not be used for the therapy, diagnostic or administration to animals including human and should not be used as food or cosmetics etc. To avoid the contamination of nuclease, nuclease-free-treated water, reagents and materials should be used. We also recommend wearing gloves and mask.
“PUREfrex® is Registered in U.S. Patent and Trademark Office”