state-of-the-art in vitro protein synthesis by
"Up to 100's of micrograms/ml in 240 minutes"
- this page: PUREfrex® Products Dashboard
- PUREfrex® Ordering Information Table
Annoucements
Posted 12 Janunary 2022
PUREfrex Product Pages
- PUREfrex 2.0
- (for protein synthesis)
- PUREfrex 2.1
(for proteins with disulfide bonds) - PUREfrex 1.0
(for basic research and mechanism studies) - PUREfrex 1.0 Custom
(formulated to your specs) - Reaction Supplements
- (EF-P, DnaK, GroE, DsbC Set, PDI Set)
PUREfrex Product Manual and Package Inserts
- PUREfrex 2.0/2.1 Kit Technical Manual
- 2.0/2.1 full version (34 pages)
- Package Inserts
- PUREfrex Kits
(reagent sets for Y ml rxn mix x number of reagent sets) - 2.0 - 0.25 mL x 1 set
2.0 - 0.25 mL x 5 sets to be discontinued
2.0 - 2 mL x 1 set new size
2.0 - 2 mL x 5 sets
2.1 - 250uL x 1 set
2.1 - 250uL x 5 sets to be discontinued
2.1 - 2mL x 1 set new size
2.1 - 2mL x 5 sets
1.0 - 250uL x 1 set
1.0 - 250uL x 5 sets to be discontinued
1.0 - 2mL x 1 set new size
1.0 - 2mL x 5 sets - PUREfrex Reaction Supplements
(reagent sets for Y ml rxn mix x number of reagent sets)
DnaK Mix - 500 uL x 1 set
DnaK Mix - 2 mL x 5 sets
GroE Mix - 500uL x 1 set
GroE Mix - 2mL x 5 sets
DsbC Set - 500uL x 1 set
DsbC Set - 2mL x 5 sets
PDI Set - 500uL x 1 set
PDI Set - 2 mL x 5 sets
EF-P - 500uL x 1 set
EF-P - 2mL x 5 sets
- PUREfrex Kits
Key Technical Notes
- ♦ PUREfrex Template Preparation
- ♦ Six Tips on PUREfrex DNA Template Design
- ♦ Synthesis of Protein Toxin (Gelonin) using DnaK Mix
- ♦ Sequence of DHFR DNA
- ♦ Influence of reducing agents on the redox state
- ♦ Improvement of specific activity by methylation of release factors (RF1, RF2).
- ♦ Improvement of the translation efficiency of consecutive proline residue-containing proteins by EF-P.
- ♦ Synthesis of proteins containing disulfide bonds with PDI and DsbC.
- ♦ Synthesis of functionally active proteins having disulfide bonds.
PUREfrex FAQ
PUREfrex in the Literature
- Citation of the Month: Jensen, et. al., Cell free protein synthesis versus yeast expression - A comparison using insulin as a model protein.
- Literature Citations
- Annotated Bibliography (pdf)
Manufacturer's Site Links
- PUREfrex at Gene Frontier
- See All FAQ, Technical Notes, Posters, and Literature Citations at GeneFrontier Sites
Safety Data Sheets
- 2.0 SDS USA EU
- 2.1 SDS USA EU
- 1.0 SDS USA EU
- DnaK Mix USA EU
- GroE Mix USA EU
- DscC Set USA EU
- PDI Set USA EU
- EF-P USA EU
Posters
- General Protein Synthesis
- Improvement of translational efficiency by N-terminal codon optimization in the reconstituted cell-free protein synthesis system [2016]
- Improvement of the translation efficiency of proline residue-containing proteins using the reconstituted cell-free protein synthesis system (PUREfrex) [2018] VIEW
- Investigation on how to synthesize active proteins by using a reconstituted cell-free protein synthesis system (PUREfrex) [2020]
- Disulfide Bonding
- Synthesis of proteins containing disulfide bonds using a reconstituted cell-free protein synthesis system (PUREfrex). [2019]
- Synthesis of proteins containing disulfide bonds using PUREfrex® with human protein disulfide isomerase [2018] VIEW
- Synthesis of functionally active proteins containing disulfide bonds using the new PURE system (PUREfrex) [2011]
- Synthesis of Immunoglobulins and Biologics
- Unique Cell-Free Protein Synthesis System, PUREfrex -Useful Platform for Protein Expression in the Development of Biologics- [2018] VIEW
- Preparation of labeled cyclic peptides with PUREfrex [2017]
- Development of a method for the synthesis of aglycosylated full-length IgG using PUREfrex [2017]
- Efficient in vitro expression of aglycosylated full-length IgG using a reconstituted cell-free protein synthesis system, PUREfrex 2.0 [2016]
- In vitro production of Fab, Toxin, and Immunotoxin using PUREfrex®2.0 with increased productivity [2015]
- Synthesis of functionally active proteins containing disulfide bonds using the new PURE system (PUREfrex) [2011]
Important Notice
Material and Preparation Method Changes
Here, we inform all users that the material for Solution I contained in PUREfrex® kits will be changed because certain materials used previously to produce Solution I have become unavailable.
After these changes, quality standards will still be met as before. But if you experience any problems in your protein synthesis, please contact us.
1. Details of Changes (three changes total)
| Product Name | Reagent Name | Reagent No.* | Reagent No.* |
| PUREfrex® 1.0 | Solution I | PF001-1 | PF001-11 |
| PUREfrex® 2.0 | Solution I | PF201-11 | PF201-12 |
| PUREfrex® 2.1 | Solution I | PF213-1 | PF213-11 |
1) For tRNAs included in Solution I of PUREfrex® 1.0, PUREfrex® 2.0 and PUREfrex® 2.1, its source and preparation method were changed.
* The reagent number can be found on the label of the tube.
2) Release factors (RF1, RF2) included in Solution II of PUREfrex® 1.0 will be change to methylated proteins with improved specific activity. This change to PUREfrex® 2.0 and PUREfrex® 2.1 was previously implemented in December 2020.
3) For Ribosomes included in Solution III of PUREfrex® 1.0, will be change to same preparation method as PUREfrex® 2.0 and PUREfrex® 2.1.
| Product Name | Reagent Name | Reagent No.*
< OLD >
|
Reagent No.*
< NEW >
|
| PUREfrex® 1.0 | Solution II | PF001-2 | PF001-21 |
| PUREfrex® 1.0 | Solution III | PF001-3 | PF-3A1 |
* The reagent number can be found on the label of the tube.
2. Applicable dates
1) Change tRNA from December 2022
2) and 3) Change RF1, RF2 and ribosome from October 2023
3. Contact information
We have an inquiry form available.