Atelocollagen Microspheres

 Atelocollagen microspheres are microbeads-like atelocollagen scaffold for cell culture. This carrier is useful for culturing adhesive cells such as fibroblasts, epithelial cells and osteoblasts due to its ability to maintain cell functions.

 

Features and Applications 

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 Mass-Culture
  • Collagen microspheres create an in vitro-like environment because they are produced exclusively from fibril-forming type I atelocollagen.
  • Cells are easily harvested by collagenase treatment.  

Type of Collagen

Bovine hide derived atelocollagen

Product

Product name (click for order info) Cat no (click for datasheet) Size
Collagen Microspheres  KOU-MIC-00  15 ml

Frequently Asked Questions

How can I seed cells onto Atelocollagen microspheres?
Please visit our distributors’ page to download an instruction manual

How can I harvest cells from Atelocollagen microspheres?
Add collagenase to final concentration of 0.1% and incubate at 37 °C for approximately an hour

Is it possible to prepare sections from Atelocollagen microspheres?
Similar to tissue samples, Atelocollagen microspheres can be fixed and/or embedded in paraffin/OCT compound.

What buffer is used with Atelocollagen microspheres?
The buffer is PBS (-).

Is it possible to obtain custom-made Atelocollagen microspheres that are different in size from the off-the-shelf product?
It could be expensive to produce custom-made Atelocollagen microspheres. We can provide you with articles that describe the fabrication of collagen beads from Collagen acidic solution instead.

Are Atelocollagen microspheres suitable for static culture?
There are several examples in the literature describing its use for static culture.

References

Bone, Tooth, Cartilage
  • microRNA-875-5p plays critical role for mesenchymal condensation in epithelial-mesenchymal interaction during tooth development.
    Funada K, Yoshizaki K, MIyazaki K, Han X, Yuta T, Tian T, Mizuta K, Fu Y, Iwamoto T, Yamada A, Takahashi I, Fukumoto S.
    Sci Rep. 2020 Mar 18;10(1):4918. PMID: 32188878
  • Strategy for the Generation of Engineered Bone Constructs Based on Umbilical Cord Mesenchymal Stromal Cells Expanded with Human Platelet Lysate.
    Silva-Cote I, Cruz-Barrera M, Cañas-Arboleda M, Correa-Araujo L, Méndez L, Jagielska J, Camacho B, Salguero G.
    Stem Cells Int. 2019 Dec 1;2019:7198215. PMID: 31885622
  • Efficacy of bone regeneration using collagen microspheres as scaffold.
    Shunsuke Baba, Naoyuki Matsumoto, Yuki Kaneshita, Kaoru lnami, Hidetoshi Morikuni and Takayoshi Kawazoe.
    J Osaka Dent Univ. 2008. 42(1), 9-15.
  • Mandibular reconstruction using a combination graft of rhBMP-2 with bone marrow cells expanded in vitro.
    Seto I, Marukawa E, Asahina I.
    Plast Reconstr Surg. 2006 Mar;117(3):902-908. PMID: 16525283
  • Propagation of human nasal chondrocytes in microcarrier spinner culture.
    Shikani AH, Fink DJ, Sohrabi A, Phan P, Polotsky A, Hungerford DS, Frondoza CG.
    Am J Rhinol. 2004 Mar-Apr;18(2):105-12. PMID: 15152876
  • The role of osteopontin on calcium oxalate crystal formation.
    Konya E, Umekawa T, Iguchi M, Kurita T.
    Eur Urol. 2003 May;43(5):564-71. PMID: 12706004
  • Collagen microcarrier spinner culture promotes osteoblast proliferation and synthesis of matrix proteins.
    Overstreet M, Sohrabi A, Polotsky A, Hungerford DS, Frondoza CG.
    In Vitro Cell Dev Biol Anim. 2003 May-Jun;39(5-6):228-34. PMID: 14613330
  • The effect of osteopontin immobilized collagen granules in the seed crystal method.
    Umekawa T, Iguchi M, Kurita T.
    Urol Res. 2001 Aug;29(4):282-6. PMID: 11585285
  • Beta-1 integrin expression by human nasal chondrocytes in microcarrier spinner culture.
    Bouchet BY, Colón M, Polotsky A, Shikani AH, Hungerford DS, Frondoza CG.
    J Biomed Mater Res. 2000 Dec 15;52(4):716-24. PMID: 11033555
  • Human chondrocytes proliferate and produce matrix components in microcarrier suspension culture.
    Frondoza C, Sohrabi A, Hungerford D.
    Biomaterials. 1996 May;17(9):879-88.　PMID: 8718933
  • Comparative histological studies of bone and cartilage formations induced by various BMP-carrier composites.:Comparative histological studies of bone and cartilage formations induced by various BMP-carrier composites.
    Liliana Missana, Noriyuki Nagai and Yoshinori Kuboki
    Japanese Association for Oral Biology 36(1), 9-19, 1994
  • Cross-linked collagen gel spheres as a useful carrier for cell culture of MC 3T3-E1 clonal osteogenic cells.
    Morimichi Mizuno, Tomoyo Kasagi, Yoshinori Kuboki.
    Japanese J. Oral Biol. 1988 30(6):855-8.

Miscellaneous

  • Selective Proliferation of Highly Functional Adipose-Derived Stem Cells in Microgravity Culture with Stirred Microspheres.
    Mashiko T, Kanayama K, Saito N, Shirado T, Asahi R, Mori M, Yoshimura K.
    Cells. 2021 Mar 4;10(3):560. PMID: 33806638
  • Type I collagen can function as a reservoir of basic fibroblast growth factor.
    Kanematsu A, Marui A, Yamamoto S, Ozeki M, Hirano Y, Yamamoto M, Ogawa O, Komeda M, Tabata Y.
    J Control Release. 2004 Sep 30;99(2):281-92. PMID: 15380637
  • Expression of vascular endothelial growth factor promotes colonization, vascularization, and growth of transplanted hepatic t issues in the mouse.
    Ajioka I, Akaike T, Watanabe Y.
    Hepatology. 1999 Feb;29(2):396-402. PMID:9918915
  • Correlation between cell-adherent activity and surface structure in Porphyromonas gingivalis.
    Watanabe K, Yamaji Y, Umemoto T.
    Oral Microbiol Immunol. 1992 Dec;7(6):357-63. PMID: 1363734