Reproductive Engineering Home
Go to: IVF and Fertility Dashboard
KYUDO/CARD IVF PRODUCTS
for Rat and Mouse
Reagents FOR MOUSE
Fertiup CP - Cryopreservation Media
Fertiup PM - Preincubation Media
CARD Media - for IVF procedure
Card HyperOva - Superovulation Reagent
CARD IVF Companion Reagents - mHTF; KSOM; DMSO; DAP213; Sucrose
Hardware Accessories - Sperm Straws, Freezing Cassettes and Canisters and more!
CARD Cold Transport Kit - for safe transport of cauda epididymis and embryos
Kyudo/CARD Reagents FOR RAT
NEW! CARD HyperOva for Rat - Legendary HyperOva superovulation performance formulated exclusively for rats.
CARD FERTIUP® Rat Sperm Cryopreservation Agent - high performance reagent for rat sperm cryopreservation
CARD Rat Sperm Freezing Kit - sperm straw holder and float to promote optimal sperm freezing
CARD Rat IVF Medium - high performance reagent for rat IVF
FLYERS and BROCHURES
Kyudo/CARD Products for RAT IVF Flyer
CARD FERTIUP® Rat Sperm Cryopreservation Agent flyer
Product Information
PROTOCOL: Rat Sperm Cryopreservation
RAT Products FAQ
Ordering Information
Instruction Manuals
CARD FERTIUP® Cryoprotectant
CARD FERTIUP® Preincubation Media
CARD MEDIUM®
CARD FERTIUP® : PM - CARD MEDIUM® Set
VIDEOS
Protocols:
Rat sperm cryopreservation protocol
Frozen rat sperm thawing protocol
Center for Animal Resources and Development (CARD), Kumamoto University
URL: https://nakagatarbi.com
2023 KYUDO
Product Catalog
Download (English)
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LEARN
CARD Technical Manual: "Reproductive Engineering Techniques in Mice" by Naomi Nakagata (3rd edition)
Journal Article: Nakagata et. al. (2020), Establishment of sperm cryopreservation and in vitro fertilization protocols for rats
Cosmo Bio Brand IVF Products
for MOUSE: - HTF; KSOM; mWM; Sucrose; DMSO; DAP213; R18S3
For RAT: - HTF; mR1ECM; Rat KSOM; SPB1; P10 PEPeS
Defined reagents for PIG: Kits; POE-CM; POM; HP-POM; dbcAMP; SEM; PFM; PZM-5; PBM
4BioDx Brand Products
AKAP4 ELISA Kits and Abs - Test fresh or frozen semen to determine sperm quality and fertility potential.
IVF and Fertility Dashboard
Reproductive Engineering and IVF: Media, Solutions, and Accessories
Products for In Vitro Fertilization (IVF) Related ProceduresWelcome to the IVF and Fertility dashboard at Cosmo Bio USA.
KYUDO/CARD IVF PRODUCTS |
Cosmo Bio Brand IVF Products |
4BioDx Brand Products |
|
A treasure box of reagents, |
* Mouse and rat IVF accessory reagents. * Porcine IVF reagents, media, and kits |
ProAKAP4 (demonstrated |
More About Our IVF Suppliers
Kyudo Product Lineup
Product Name | Size | Catalog Number |
CARD FERTIUP Cryoprotectant: CPA |
500 ul | KYD-001-05-EX |
25 x 500 ul | KYD-001-05-EX-X25 | |
5 x 500 ul | KYD-001-05-EX-X5 | |
50 x 500 ul | KYD-001-05-EX-X50 | |
1 ml | KYD-001-EX | |
25 x 1 ml | KYD-001-EX-X25 | |
5 x 1 ml | KYD-001-EX-X5 | |
50 x 1 ml | KYD-001-EX-X50 | |
CARD FERTIUP Preincubation Medium: PM |
500 ul | KYD-002-05-EX |
25 x 500 ul | KYD-002-05-EX-X25 | |
5 x 500 ul | KYD-002-05-EX-X5 | |
50 x 500 ul | KYD-002-05-EX-X50 | |
1 ml | KYD-002-EX | |
25 x 1 ml | KYD-002-EX-X25 | |
5 x 1 ml | KYD-002-EX-X5 | |
50 x 1 ml | KYD-002-EX-X50 | |
CARD MEDIUM |
1 Kit | KYD-003-EX |
10 Kits | KYD-003-EX-X10 | |
5 Kits | KYD-003-EX-X5 | |
CARD FERTIUP PM 1 mL - CARD MEDIUM Set |
1 Kit | KYD-004-EX |
10 Kits | KYD-004-EX-X10 | |
5 Kits | KYD-004-EX-X5 | |
CARD FERTIUP PM 0.5 mL - CARD MEDIUM Set |
1 Kit | KYD-005-EX |
10 Kits | KYD-005-EX-X10 | |
5 Kits | KYD-005-EX-X5 | |
CARD Cold Transport Kit | 1 Set | KYD-006-EX |
Cold storage solution for cauda epididymides |
1 Kit | KYD-007-EX |
10 Kits | KYD-007-EX-X10 | |
5 Kits | KYD-007-EX-X5 | |
CARD mHTF (modified Human Tubal Fluid) |
2 ml | KYD-008-02-EX |
25 x 2 ml | KYD-008-02-EX-X25 | |
5 x 2 ml | KYD-008-02-EX-X5 | |
50 x 2 ml | KYD-008-02-EX-X50 | |
5 ml | KYD-008-05-EX | |
15 x 5 ml | KYD-008-05-EX-X15 | |
3 x 5 ml | KYD-008-05-EX-X3 | |
30 x 5 ml | KYD-008-05-EX-X30 | |
CARD HyperOva (Superovulation Reagent for mouse) |
600 ul | KYD-010-06-EX |
25 x 600 ul | KYD-010-06-EX-X25 | |
5 x 600 ul | KYD-010-06-EX-X5 | |
50 x 600 ul | KYD-010-06-EX-X50 | |
1 ml | KYD-010-EX | |
25 x 1 ml | KYD-010-EX-X25 | |
5 x 1 ml | KYD-010-EX-X5 | |
50 x 1 ml | KYD-010-EX-X50 | |
CARD KSOM |
2 ml | KYD-011-EX-2 |
5 ml | KYD-011-EX-5 | |
CARD 1M DMSO | 1 ml | KYD-012-EX |
CARD DAP213 |
500 ul | KYD-013-EX-0.5 |
1 ml | KYD-013-EX-1 | |
CARD 0.25M Sucrose |
2 ml | KYD-014-EX-2 |
5 ml | KYD-014-EX-5 | |
CARD HyperOva FD |
1 x 2 ml | KYD-015-EX |
25 x 2 ml | KYD-015-EX-X25 | |
5 x 2 ml | KYD-015-EX-X5 | |
50 x 2 ml | KYD-015-EX-X50 | |
CARD Fertiup Rat Sperm Cryopreservation Agent |
1.5 ml | KYD-FR-001 |
10 x 1.5 ml | KYD-FR-001-X10 | |
5 x 1.5 ml | KYD-FR-001-X5 | |
CARD Rat IVF Medium |
1 ml | KYD-FR-002 |
10 x 1 ml | KYD-FR-002-X10 | |
5 x 1 ml | KYD-FR-002-X5 | |
CARD Rat Freezing Kit | 1 Kit | KYD-GAR-001 |
Reproductive Engineering Techniques in Mice Technical Manual | 1 Piece | KYD-MANUAL |
Freezing Canister | 1 Unit | KYD-S018 |
Sperm Straws (10 Pieces x 10 Units) | 10 Pieces | KYD-S020X10 |
Triangular Cassette Short (10units) | 10 Units | KYD-S021 |
Straw Connector | 1 Piece | KYD-S025 |
Triangular Cassette Long (10units) | 10 Units | KYD-S035 |
Embryo manipulation instrument set | 1 Set | KYD-S036 |
Glass capillary 20PC | 1 Set | KYD-S037 |
Kyudo's products anchor Cosmo Bio USA's IVF and Fertility product category. In collaboration with the Center for Animal Resources and Development (CARD) at Kumamoto University, Kyudo products are recognized worldwide for quality and performance. Their innovative reagents for boosting the efficiencies of mouse sperm cryopreservation, superovulation, and IVF have contributed greatly to the worldwide increase in strain preservation via sperm cryopreservation.
Superovulation reagent HyperOVA® is now also available in a freeze-dried format. HyperOva® FD delivers the same great performance but ships and stores at 4 C instead of dry ice and -80 C.
CARD FERTIUP® Rat Sperm Cryopreservation Agent and CARD MEDIUM® for RAT IVF!
Cosmo Bio offers our own brand of high performance IVF-related reagents with a focus on convenience and value. We offer an assortment of accessory reagents for mouse and rat IVF-related procedures in sizes that may be most compatible with your laboratory's work flow. Our reagent quality and consistency are assured. Cosmo Bio's IVF reagents are produced and packaged in Japan under ISO9001 global quality management standards. All reagents are sealed in glass ampoules and store at 4 degrees C with shelf lives of at least one year from the date of manufacture.
For porcine IVF, Cosmo Bio offers a range of IVF reagents developed over more than 10 years by academic researchers and Japan's Institute for Functional Peptides with a focus on high performance animal-free, defined media for the production of high quality embryos.
Cosmo Bio Product Lineup
Product Name | Size | Catalog Number |
DAP213 (DMSO, Acetamide, Propylene Glycol) | 10 x 1 ml | CSR-R-T073 |
DMSO 1M | 10 x 2 ml | CSR-R-T072 |
HTF (Human Tubal Fluid) |
10 x 2 ml | CSR-R-B070 |
10 x 5 ml | CSR-R-B071 | |
M2 |
10 x 2 ml | CSR-R-M083 |
10 x 5 ml | CSR-R-M084 | |
Modified Krebs-Ringer Bicarbonate (mKRB) medium | 100 ml | CSR-R-M193 |
Mouse KSOM (Potassium Simplex Optimized Medium) |
10 x 2 ml | CSR-R-B074 |
10 x 5 ml | CSR-R-B075 | |
mWM (modified Whitten’s Medium) |
10 x 2 ml | CSR-R-B080 |
10 x 5 ml | CSR-R-B081 | |
P10 (Propylene Glycol in PB1) | 10 x 2 ml | CSR-R-P186 |
PB1 |
10 x 2 ml | CSR-R-P185 |
10 x 5 ml | CSR-R-P138 | |
PEPeS (Percoll in PB1) | 10 x 1 ml | CSR-R-P187 |
R18S3 (Medium for Mouse Sperm Cryopreservation) | 10 x 1 ml | CSR-R-R082 |
Rat KSOM (Potassium Simplex Optimized Medium) | 10 x 1 ml | CSR-R-R148 |
SPB1 |
10 x 2 ml | CSR-R-S187 |
10 x 5 ml | CSR-R-S188 | |
Sucrose 0.25M |
10 x 2 ml | CSR-R-Y077 |
10 x 5 ml | CSR-R-Y078 |
A major focus of 4BioDx (Lille, France) is the development and commercialization or reagents and ELISA kits to measure proAKAP4 concentration in semen. proAKAP4 is an indispensable molecule during spermatogenesis and any modification of proAKAP4 expression during spermatogenesis or modification in its metabolism will have consequences on sperm motility, hyper motility, sperm capacitation and then fertility. Consequently, measurement of proAKAP4 in sperm is an increasingly promising approach to evaluate semen quality prior to in vitro fertilization in both laboratory animals and commercial livestock.
4BioDX Product Lineup
Catalog Number | Product Name | Size | Clone No | Applications | Clonality | Host | Reactivity |
BDX-4BDX-1503 | Anti-APP [pT668] pAb | 80ul | IHC, IF, WB | Polyclonal | Rabbit | Rat, Mouse, Human | |
BDX-4BDX-1503S | Anti-APP [pT668] pAb | 40ul | IHC, IF, WB | Polyclonal | Rabbit | Rat, Mouse, Human | |
BDX-4BDX-1602 | Anti-AKAP4 mAb (clone 7E10) | 100ug | 7E10 | IEM, ELISA, IF | Monoclonal | Mouse | Sheep, Bovine, Equine, Porcine, Human |
BDX-4BDX-1602S | Anti-AKAP4 mAb (clone 7E10) | 20ug | 7E10 | IEM, ELISA, IF | Monoclonal | Mouse | Sheep, Bovine, Equine, Porcine, Human |
BDX-4BDX-1701 | Anti-pro AKAP4 mAb (clone 6F12) | 100ug | 6F12 | IEM, ELISA, IF | Monoclonal | Mouse | Sheep, Bovine, Equine, Porcine, Human |
BDX-4BDX-1701S | Anti-pro AKAP4 mAb (clone 6F12) | 20ug | 6F12 | IEM, ELISA, IF | Monoclonal | Mouse | Sheep, Bovine, Equine, Porcine, Human |
BDX-4BDX-1805 | Anti-AKAP4 mAb (clone 10F8) | 100ug | 10F8 | IF, IHC, WB | Dog, Goat, Porcine, Rabbit | ||
BDX-4BDX-1805S | Anti-AKAP4 mAb (clone 10F8) | 20ug | 10F8 | IF, IHC, WB | Dog, Goat, Porcine, Rabbit | ||
BDX-4BDX-18K12RUO | proAKAP4 ELISA Kit (Rodent & Human) | 1Kit | Human, Mouse, Rat, Guinea Pig, Hamster | ||||
BDX-4BDX-18K8 | proAKAP4 4MID Kit (ELISA, Mouse) | 1Kit | Mouse | ||||
BDX-4BDX-18K8BB | proAKAP4 4MID BB Kit (ELISA, Mouse) | 1Kit | Mouse | ||||
BDX-4BDX-1901 | Anti-pro AKAP4 mAb (clone 5H2) | 100ug | 5H2 | IF, IHC, FC, WB | Human, Mouse, Porcine | ||
BDX-4BDX-1901S | Anti-pro AKAP4 mAb (clone 5H2) | 20ug | 5H2 | IF, IHC, FC, WB | Human, Mouse, Porcine | ||
BDX-4VDX-18K2 | proAKAP4 4MID Kit (ELISA, Pig) | 1Kit | Porcine | ||||
BDX-4VDX-18K3 | proAKAP4 4MID Kit (ELISA, Horse) | 1Kit | Equine | ||||
BDX-4VDX-18K3BB | proAKAP4 4MID BB Kit (ELISA, Horse) | 1Kit | Equine | ||||
BDX-4VDX-18K4 | proAKAP4 4MID Kit (ELISA, Bull) | 1Kit | Bovine | ||||
BDX-4VDX-18K5 | proAKAP4 4MID Kit (ELISA, Dog) | 1Kit | Dog | ||||
BDX-4VDX-18K5BB | proAKAP4 4MID BB Kit (ELISA, Dog) | 1Kit | Dog | ||||
BDX-4VDX-18K6 | proAKAP4 4MID Kit (ELISA, Rabbit) | 1Kit | Rabbit | ||||
BDX-4VDX-18K7 | proAKAP4 4MID Kit (ELISA, Ram) | 1Kit | Ram | ||||
BDX-4VDX-18K9 | proAKAP4 4MID Kit (ELISA, Goat) | 1Kit | Goat | ||||
BDX-4VDX-19K10 | proAKAP4 4MID Kit (ELISA, Feline) | 1Kit | Feline | ||||
BDX-4VDX-19K11 | proAKAP4 4MID Kit (ELISA, Camel) | 1Kit | Camel |