Milk Exosome Antibody

Polyclonal antibody produced from exosomes purified from bovine milk by ultracentrifugation as an antigen.

Exosomes are membrane vesicles with diameters ranging from 50 nm to 150 nm that are secreted by most cells. Exosomes have been observed in vivo in saliva, blood, urine, amniotic fluid, malignant ascites fluid, and other body fluids, and have also been found secreted by cells in culture. Although it has been suggested that these exosomes encapsulate miRNAs, mRNAs, proteins, and micropeptides and play a role in transmitting information to other target cells and tissues far away from the cell or tissue of origin¹⁾, their target orientation is not yet completely clear.On the other hand, it has been shown that neutralization of exosome signaling with exosome surface antigen-specific antibodies such as CD9 and CD63 can inhibit cancer metastasis²⁾.

It has been suggested that exosomes also exist in milk of a wide range of animals, including humans, cows, and rats, and that they function by internalizing miRNAs, mRNAs, and proteins, which are then taken up by target cells³⁾.

In addition, recent studies have utilized the aforementioned properties of exosomes as drug delivery carriers (DDS), and in particular, exosomes derived from raw milk are attracting attention as a means of orally administering anticancer drugs⁴⁾.

Specification

Class	:Rabbit Polyclonal
Immunogen	:Bovine Exosome
Reactivity	:Bovine
Specificity	:Whole Exosome of bovine Milk
Purification	:Ammonium sulfate precipitation Milk Whey protein absorbed
Buffer	:PBS(-) 15ppm Proclin300
Application	:WB

Data

Western blotting using Anti Milk Exosome Antibody (EXO-AB-01)

1 µg of bovine milk exosome was detected with this antibody diluted 1000-fold.

References

1. Tumour exosome integrins determine organotropic metastasis, Hoshino A, et al., Nature. 2015 Nov 19; 527(7578): 329-35
2. Disruption of Circulating Extracellular Vesicles as a Novel Therapeutic Strategy against Cancer Metastasis, Nao Nishida-Aoki, et al., Mol Ther. 2017 Jan 4; 25(1): 181-191.
3. Bovine milk exosomes contain microRNA and mRNA and are taken up by human macrophages, Hirohisa Izumi, et al., J Dairy Sci. 2015 May; 98(5): 2920-33
4. Milk-derived exosomes for oral delivery of paclitaxel, Ashish K.Agrawal et al., Nanomedicine. 2017 Jul; 13(5): 1627-1636.

Product List

Milk Exosome Antibody

Product name	Catalog Number
Anti Milk Exosome, Bovine (Rabbit)	CAC-EXO-AB-01