High Quality anti-Human Tetraspanin Monoclonal Antibodies
This product is an antibody that specifically recognizes CD9, CD63, and CD81, known as exosome markers, and can isolate exosomes from serum and culture supernatants by immunoprecipitation.
Features
- ●High Specificity for tetraspanin proteins, CD9, CD63 and CD81
- ●Sample Type (Human samples)
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- CD9 : serum, plasma, Cell culture supernatants and urine
- CD63 : serum, plasma, Cell culture supernatants and urine
- CD81 : serum, plasma and Cell culture supernatants
- ●CD9 Antibody was tedted with bovine milk exosomes and CD81 antibody was tested with bovine milk exosomes and FBS.
- ●Analyisis of exosome surface antigens, exosomal miRNAs and proteins included in exosomes
Data
IP-WB
- 1. IP-WB of CD9 in serum Exosome with anti CD9 (12A12) antibody
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1. CD9 antibody (12A12) Serum A 2. control antibody Serum A 3. CD9 antibody (12A12) Serum B 4. control antibody Serum B 5. ultracentrifugation Serum A 6. ultracentrifugation Serum B
- 2. IP-WB of CD63 in serum Exosome with anti CD63 (8A12) antibody
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1. CD63 antibody (8A12) Serum A 2. control antibody Serum A 3. CD63 antibody (8A12) Serum B 4. control antibody Serum B 5. ultracentrifugation Serum A 6. ultracentrifugation Serum B
- 3. IP-WB of CD81 in serum Exosome with anti CD81 (12C4) antibody
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1. CD81 antibody (12C4) Serum A 2. control antibody Serum A 3. CD81 antibody (12C4) Serum B 4. control antibody Serum B 5. ultracentrifugation Serum A 6. ultracentrifugation Serum B
Immunofluorescent Cell Staining (ICC / IF)
![](https://cdn11.bigcommerce.com/s-ydswqc5qsc/images/stencil/original/image-manager/cd9cd63cd81-04.png)
- A: prostate cancer cell line, PC-3M
- B: colon cancer cell line, HCT116
Secondary antibody is Alexa Fluor(R) 488-labeled anti mouse secondary antibody.
National Cancer Center Research Institute of Molecular and Cellular Therapy
Tokyo Medical University
Immunoelectron Microscopy
![](https://cdn11.bigcommerce.com/s-ydswqc5qsc/images/stencil/original/image-manager/cd9cd63cd81-05.png)
- (A) Anti-human CD9 antibody (clone 12A12) detected CD9 on the surface of exosomes from human breast cancer cells.
- (B) Anti-human CD63 antibody (clone 8A12) also detected CD63 on the surface of exosomes from human breast cancer cells.
National Cancer Center Research Institute of Molecular and Cellular Therapy
Cryo-electron microscopy
![](https://cdn11.bigcommerce.com/s-ydswqc5qsc/images/stencil/original/image-manager/cd9cd63cd81-06.png)
![](https://cdn11.bigcommerce.com/s-ydswqc5qsc/images/stencil/original/image-manager/cd9cd63cd81-07.png)
Exosomes and anti-human CD63 antibody (clone 8A12) were mixed and imaged with cryo-electron microscopy (Titan Krios). Shown is a cryo-electron micrograph of an exosome (A), a three-dimensional reconstruction of an exosome (green) with a single bound antibody (cyan) (B) and a rotating 3D tomographic reconstruction of an exosome and a bound antibody (C).
Okinawa Protein Tomography Ltd.
Specification
Target | Clone | Immunogen | Reactive | Non-reactive | Application | Isotype |
---|---|---|---|---|---|---|
CD9 | 12A12 | Amino acids 113-195: the large second extracellular loop between the 3rd and 4th transmembrane domains | Human | Mouse, Rat | IP, WB, FC, ELISA, IF, IHC, IEM | IgG2b |
CD63 | 8A12 | Amino acids 104-202: the large second extracellular loop between the 3rd and 4th transmembrane domains | Human | Mouse, Rat | IP, WB, FC, ELISA, IF, IHC, IEM | IgG2a |
CD81 | 12C4 | Amino acids 36-54: the small first extracellular loop between the 3rd and 4th transmembrane domains | Human, Bovine | Mouse, Rat | IP, WB, FC, ELISA, IF, IHC, IEM | IgG2a |
*CD9 antibody and CD63 antibody were used in ExoScreen assay and In vitro opsonization assay. CD9 antibody was used in in vivo metastasis assay and mass spectrometric immunoassay with monolith pipette tips. CD63 antibody was used in Membrane fusion assay.
User Reports
Product List
Anti-Human Tetraspanin Monoclonal Antibodies
Product name | Catalog Number |
---|---|
Anti CD9, Human (Mouse) Unlabeled, 12A12 | SHI-EXO-M01 |
Anti CD63, Human (Mouse) Unlabeled, 8A12 | SHI-EXO-M02 |
Anti CD81, Human (Mouse) Unlabeled, 12C4 | SHI-EXO-M03 |
Fluorescent-labeled Anti-Human Tetraspanin Monoclonal Antibodies
Tide Fluor™ 2 (TF2) has spectral properties similar to those of fluorescein and Alexa Fluor® 488 (Invitrogen). Compared to fluorescein, TF2 has much stronger fluorescence and higher photostability. Additionally, its fluorescence is pH-independent from pH 3 to 11. TF2-labeled antibodies exhibit much stronger fluorescence and higher photostability than those labeled with FITC. These characteristics make TF2 a superior alternative to fluorescein.
Tide Fluor™ 5 (TF5) has spectral properties essentially identical to those of Cy5. Compared to Cy5, TF5 has much stronger fluorescence and higher photostability. Additionally, its fluorescence is pH-independent from pH 3 to 11. TF5-labeled antibodies exhibit much stronger fluorescence and higher photostability than those labeled with Cy5. These characteristics make TF5 a superior alternative to Cy5.
Product name | Catalog Number |
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Anti CD9, Human (Mouse) Label Tide Fluor™ 2WS, 12A12 | SHI-EXO-M01-TF2 |
Anti CD9, Human (Mouse) Label Tide Fluor™ 5 WS, 12A12 | SHI-EXO-M01-TF5 |
Anti CD63, Human (Mouse) Label Tide Fluor™ 2WS, 8A12 | SHI-EXO-M02-TF2 |
Anti CD63, Human (Mouse) Label Tide Fluor™ 5WS, 8A12 | SHI-EXO-M02-TF5 |
Anti CD81, Human (Mouse) Label Tide Fluor™ 2WS, 12C4 | SHI-EXO-M03-TF2 |
Anti CD81, Human (Mouse) Label Tide Fluor™ 5WS, 12C4 | SHI-EXO-M03-TF5 |
Biotin-labeled Anti-Human Tetraspanin Monoclonal Antibodies
Product name | Catalog Number |
---|---|
Anti CD9, Human (Mouse) Biotin, 12A12 | SHI-EXO-M01-B |
Anti CD63, Human (Mouse) Biotin, 8A12 | SHI-EXO-M02-B |
Anti CD81, Human (Mouse) Biotin, 12C4 | SHI-EXO-M03-B |
Citations
- ■CD9 antibody
- S Tsuda et al., Sci Rep. 2017 Oct 11;7(1):12989. doi: 10.1038/s41598-017-13154-0.
- N Nishida-Aoki et al., Mol Ther. 2017 Jan 4;25(1):181-191. doi: 10.1016/j.ymthe.2016.10.009.
- K Matsuzaki et al., Oncotarget. 2017 Apr 11; 8(15): 24668-24678. doi:10.18632/oncotarget.14969
- Kazutoshi Fujita et al., Sci Rep. 2017; 7: 42961. doi: 10.1038/srep42961
- Yoshioka Y et al., Nat Commun. 2014 Apr 7;5:3591. doi: 10.1038/ncomms4591.
- Saito S et al., Sci Rep. 2018 Mar 5;8(1):3997. doi: 10.1038/s41598-018-22450-2.
- Yagi Y et al., Neurosci Lett. 2017 Jan 1;636:48-57. doi: 10.1016/j.neulet.2016.10.042. Epub 2016 Oct 22.
- Ueda K et al., Sci Rep. 2014 Aug 29;4:6232. doi: 10.1038/srep06232.
- Tokuoka, S. M. et al. Lipid Profiles of Human Serum Fractions Enhanced with CD9 Antibody-Immobilized Magnetic Beads. Metabolites 12, 230 (2022).
- ■CD63 antibody
- N Nishida-Aoki et al., Mol Ther. 2017 Jan 4;25(1):181-191. doi: 10.1016/j.ymthe.2016.10.009.
- Yoshioka Y et al., Nat Commun. 2014 Apr 7;5:3591. doi: 10.1038/ncomms4591.
- Saito S et al., Sci Rep. 2018 Mar 5;8(1):3997. doi: 10.1038/s41598-018-22450-2.
- ■CD81 antibody
- M Somiya et al., J Extracell Vesicles. 2018 Feb 21;7(1):1440132. doi:10.1080/20013078.2018.1440132. eCollection 2018.
- Takahashi A et al., Nat Commun. 2017 May 16;8:15287. doi: 10.1038/ncomms15287.