ExoTrap™ Exosome Isolation Spin Column Kit for Protein Research

Spin column type exosome isolation kit

ExoTrap™ Exosome Isolation Spin Column immobilizes a human CD9 antibody known as an exosome marker on the spin column and extracts human serum, plasma, saliva, urine, culture supernatant from Western blot, mass spectrometry, etc. within 30 minutes. Exosome-derived proteins that can be used for protein research can be isolated .

Background

Exosomes are vesicles with a diameter of about 50 nm to 150 nm formed by a lipid bilayer membrane secreted from cells. In vivo, it is observed in body fluids such as saliva, blood, urine, amniotic fluid, and malignant ascites, and is also secreted from cultured cells. In recent years, it has been reported that exosomes contain various proteins and RNA, and it has been pointed out that exosomes may play a role in transmitting information between cells.

Features

• High-purity exosomes can be isolated from serum, plasma, urine, saliva, and culture supernatant (tested with human samples only) within 30 minutes.
• It is a familiar spin column type.

Experimental example by Western blot

After recovering exosome-derived proteins with 50uL of SDS sample buffer using ExoTrap™, the expression of exosome markers was confirmed by Western blotting.

1. Exosome isolation from culture supernatant

Sample: 293T cell culture supernatant,
MKN7 Primary antibody: Anti CD9 (#SHI-EXO-M01), Anti CD63 (#SHI-EXO-M02), Anti CD81 (#SHI-EXO-M03)
amount applied : 20uL / lane

　 　 

2. Exosome isolation from human plasma

Sample: Human plasma treated with EDTA
Primary antibody: Anti CD9 (#SHI-EXO-M01)
Amount applied: 20uL / lane

Catalog Number	Product Name	Size
CSR-SHI-EXO-K010	ExoTrap Exosome Isolation Spin Column Kit for protein research	10 Preps