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Cell / Cell culture products

Epididymal adipocytes(rats and mice)

Useful for drug discovery for lifestyle-related diseases and development of preventive foodsPeri-epididymal adipocytes contain collagenase-treated adipose precursor cells isolated from rat or mouse peri-epididymal adipose tissue. It can be used to search for therapeutic drugs for lifestyle-related diseases and to develop preventive foods.

Special issue on lifestyle-related diseases

Background

There are separate areas of fatty tissue in the abdominal cavity, one of which is the fatty tissue surrounding the epididymis (epididymis). This product is pre-adipocytes isolated from adipose tissue around the rat epididymis. Please use the dedicated adipocyte differentiation medium.
PMC_EAC01C_1.jpg

Figure 1 Mouse epididymal adipocytes (nuclear staining)

Intended use

Using this culture system, it is possible to search for therapeutic drugs for obesity, diabetes, hypertension, and arteriosclerosis, develop foods to prevent lifestyle-related diseases, perform functional test lipid metabolism experiments on anti-obesity functional foods, and screen new β 3 agonists. is.

Composition

organization  animal  age in weeks  Composition  Catalog number 
 epididymal fat ICR mouse   mature animal Epididymal preadipocytes (mouse, frozen cells) 1.5 × 10 6 cells 1 cell PMC-EAC11C

Preparation method

Preadipocyte cells isolated from adipose tissue around the epididymis of rat or mouse by collagenase treatment.

Epididymal adipocytes (frozen cells)

Product name Catalog number packaging
Rat epididymal adipocyte/  Epididymal Adipocyte , Rat PMC-EAC01C 1 VIAL [3*10 6 cells x 1]
Mouse epididymal adipocyte/  Epididymal Adipocyte , Mouse PMC-EAC11C 1 VIAL[1.5 x 106 cells]
  • If the frozen cells will not be used immediately after receipt, store them in liquid nitrogen (or below -70°C).
  • Please use the cells together with the dedicated medium.

Dedicated medium

It is a serum-containing medium whose ingredients have been adjusted so that perididymal adipocytes can be induced to differentiate from preadipocytes to adipocytes.
Natural lipids are added as adipocyte differentiation inducers. Under these culture conditions, more than 80% of the cells differentiate into adipocytes without the addition of differentiation-inducing factors such as chemically synthesized products such as indomethacin, dexamethasone, and PPAR-γ agonists.

Product name Catalog number Size
Epididymal Adipocyte  Culture Medium PMC-EACMR 250ML

Publications

  1. Yoshida, H., Takamura, N., Shuto, T., Ogata, K., Tokunaga, J., Kawai, K., Kai, H.
    The Citrus Flavonoids Hesperetin and Naringenin Block the Lipolytic Actions of TNF-a in Mouse Adipocytes.
    Biochem. Biophys. Res. Commun. 394, 728-732 (2010), (PMID: 20230793 )