Cell / Cell culture products

1st strand cDNA (rat/mouse pancreas)

For usage as a template for RT-PCR

 

Pancreas is a tissue with high RNase expression, making it difficult to prepare high-purity RNA.
This product is reverse transcribed using oligo(dT) primer and random primer with 0.5 μg of total RNA prepared from rat (SD rat 7-10 weeks old) or mouse (ICR mouse 7-10 weeks old) pancreas as a template. It becomes the reacted 1st strand cDNA.

Features

• cDNA prepared using the RevertAid™ First Strand cDNA Synthesis Kit (Fermentas) is diluted 10-fold with sterile water.
• It can be used as a template for real-time PCR, such as expression analysis of various genes.
  * This product is not a full-length cDNA. Please note that it may not be suitable for cloning.
• The volume required for one reaction system (20 μl) is 0.125 μl (Insulin, rRNA, etc.) to 2.5 μl (beta-actin, Pdx-1, etc.). please give me.

Experimental example

Agarose gel analysis results after amplification by PCR using mouse pancreas cDNA as a template.

Lane 1: β -Actin PCR product (267 bp)
Lane 2: Insulin1 PCR product (210 bp)
Lane 3: Insulin2 PCR product (392 bp)
Lane 4: Pancreas duodenum homeobox (Pdx) 1 PCR product (371 bp) )
Lane 5: Sulfonylurea receptor (SUR) 1 PCR product (233 bp)
Lane 6: Inward rectifying potassium channel (Kir6.2) PCR product (417 bp) Lane
7: Glucose transporter 2 PCR product (267 bp)
Reaction system: 20 μl
Template amount: 2 μl (Ins2 only: 0.2 μl)
Number of cycles: 30 cycles (Ins2 and β -Actin only: 25 cycles)

1st strand cDNA (rat/mouse pancreas)

Product name	Catalog number	Size
Rat Pancreatic cDNA/  Pancreas cDNA (first strand) , Rat	PMC-RPCDNA	50 UL [equivalent to 0.5 μg total RNA]
Rat Pancreas cDNA (dT)/  Rat Pancreas cDNA(dT)	PMC-RPCDNA-DT	50UL
Mouse Pancreas cDNA/  Pancreas cDNA (first strand) , Mouse	PMC-MPC-DNA	50 UL [equivalent to 0.5 μg total RNA]
Mouse Pancreas cDNA (dT)/  Pancreas cDNA (dT) , Mouse	PMC-MPCDNA-DT	50UL