Osteogenesis Culture kit

Useful for evaluate osteogenesis ability

There are hematopoietic stem cells and bone marrow stromal cells in bone marrow. Bone marrow stromal cells contain undifferentiated mesenchymal stem cells that can differentiate into a variety of cell types such as osteoblasts chondrocytes adipocytes and so on. The Osteogenesis Culture Kit contains cryopreserved cells isolated from mouse bone marrow and two types of culture medium. The cells in this product can be grown using Growth Medium (Code No. PMC- OGCMG), and then can be differentiated into mature osteoblasts, which form calcified nodules, using Culture Medium (Mouse) (Code No. PMC- OGCMO).

Components

• ●Bone marrow stromal cell, Mouse
• ●Growth medium
• ●Culture medium

Strain of cells

ICR Mouse Bone Marrow

Main ingredient of Media

Media	Main ingredient
Growth medium	α-MEM, FBS, penicillin and streptomycin
Culture medium	α-MEM, FBS, penicillin, streptomycin, dexamethasone, ascorbic acid and β-Glycerophosphate.

Data

Figure 1. Morphology of cultured cells

A) Day 0, B) confluent, C) 3 weeks after medium is changed into “Osteogenesis Culture Medium for Osteogenesis Culture kit” (35 mm dish)

Figure 2. Cells are cultured according to protocol and subjected to activity staining of alkaline phosphatase or calcified nodules. The activity of alkaline phosphatase is detected by Alkaline Phosphatase Staining kit (Code: PMC-AK20) and the calcium deposit is detected by Calcified nodule Staining kit (Code: PMC-AK21).

Product List

Osteogenesis Culture kit

Product name	Catalog Number
Osteogenesis Culture kit, Mouse	PMC-OGC11

Optimized Medium

Component-conditioned medium containing serum to promote cell proliferation and osteogenesis, respectively.

Product name	Catalog Number
Osteogenesis Growth Medium	PMC-OGCMG
Osteogenesis Culture Medium	PMC-OGCMO

Alkaline Phosphatase Staining kit

Alkaline Phosphatase Staining Kit is designed for the rapid and sensitive detection of osteoblasts differentiated in culture from bone marrow cells.

Product name	Catalog Number
Alkaline Phosphatase Staining Kit	PMC-AK20

Calcified nodule Staining kit

The Calcified nodule Staining kit contains Substrate-containing Buffer composed mainly of Alizarin Red S and Chromogenic Substrate, and provides a convenient system for staining calcified nodule with ease.

Product name	Catalog Number
Calcified Nodule Staining kit	PMC-AK21