Osteoclasts
Bone metabolism is composed of balanced osteogenesis and bone resorption. Research studies have shown that bone marrow cells can be differentiated into osteoclasts using M-CSF (Macrophage Colony Stimulating Factor) and RANKL (Receptor Activator of NF kappa B Ligand).
Human Osteoclast cells
Osteoclast cells (human) are bone marrow mononuclear cell-derived osteoclast precursor cells.
■Data
Fig.1 Osteoclast inducted of differentiation with M-CSF/RANKL 
Fig.2 TRAP staining of human osteoclast 
Fig.3 Pit on the slice of ivory. (HE staining) 
Fig4.Pit on the slice of ivory. (HE staining) 
Fig.5 Pit on the slice of ivory. (SEM picture) Product List
■Human Osteoclast cells
| Product name | Catalog Number |
|---|---|
| Human Osteoclasts | PMC-OSC15C |
Notes
When cells have been properly stored in liquid nitrogen and cultured according to instructions using recommended media and reagents, we guarantee against poor growth at the initiation of culture. For product support, please contact us (email: primarycell@cosmobio.co.jp). The warranty period is [within 6 months after receiving the product]. Warranty is not applicable if changes are made to the media or its usage, or if refrozen cells are utilized.
■Optimized Medium
For guaranteed optimal recovery and differentiation we recommend Osteoclast Wash Medium (PMC-OSCMW) and CSF/RANKL-containing Osteoclast Culture Medium (for human) (PMC-OSCMHB).
| Product name | Catalog Number |
|---|---|
| Osteoclast Culture Medium (for human) | PMC-OSCMHB |
| Osteoclast Wash Medium | PMC-OSCMW |
Mouse Osteoclast cells
Bone marrow-derived osteoclast precursor cells from ICR mouse
Citations
- Kurinami, H., Shimamura, M., Nakagami, H., Shimizu, H., Koriyama, H., Kawano, T., Wakayama, K., Mochizuki, H., Rakugi, H., Morishita, R. A Novel Therapeutic Peptide as a Partial Agonist of RANKL in Ischemic Stroke. Sci. Rep. 6, 38062 (2016), (PMID:27897273)
- Yano, K., Washio, K., Tsumanuma, Y., Yamato, M., Ohta, K., Okano, T., Izumi, Y. The role of Tsukushi (TSK), a small leucine-rich repeat proteoglycan, in bone growth. Regen. Ther. 7, 98-107 (2017), (PMID:30271858)
- Shimamura, M., Nakagami, H., Shimizu, H., Mukai, H., Watanabe, R., Okuzono, T., Kawano, T., Ikeda, Y., Hayashi, H., Yoshida, S., Ju, N., Mochizuki, H., Morishita, R. Development of a novel RANKL-based peptide, microglial healing peptide1-AcN (MHP1-AcN), for treatment of ischemic stroke. Sci. Rep. 8, 17770 (2018), (PMID:30538259)
Product List
■Mouse Osteoclast cells
| Product name | Catalog Number |
|---|---|
| Mouse Osteoclasts | PMC-OSC14C |
Notes
When cells have been properly stored in liquid nitrogen and cultured according to instructions using recommended media and reagents, we guarantee against poor growth at the initiation of culture. For product support, please contact us (email: primarycell@cosmobio.co.jp). The warranty period is [within 6 months after receiving the product]. Warranty is not applicable if changes are made to the media or its usage, or if refrozen cells are utilized.
■Optimized Medium
Rat Osteoclast cells
Bone marrow-derived osteoclast precursor cells from SD rat
Citations
- Nakatake, H., Ekimoto, H., Aso, M., Ogawa, A., Yamaguchi, A., Suemune, H. Dialkyl bisphosphonate platinum(II) complex as a potential drug for metastatic bone tumor. Chem. Pharm. Bull. (Tokyo). 59, 710-3 (2011), (PMID:21628905)
- Nagaie, M., Nishiuram A., Hondam Y., Fujiwaram S., Matsumotom N. A comprehensive mixture of tobacco smoke components retards orthodontic tooth movement via the inhibition of osteoclastogenesis in a rat model. Int. J. Mol. Sci. 15, 18610-22 (2014), (PMID:25322153)
- Ogata, K., Katagiri, W., Osugi, M., Kawai, T., Sugimura, Y., Hibi, H., Nakamura, S., Ueda, M. Evaluation of the therapeutic effects of conditioned media from mesenchymal stem cells in a rat bisphosphonate-related osteonecrosis of the jaw-like model. Bone. 74, 95-105 (2015), (PMID:25613174)
- Li, P., Honda, Y., Arima, Y., Yasui, K., Inami, K., Nishiura, A., Hashimoto, Y., Matsumoto, N. Interferon-γ enhances the efficacy of autogenous bone grafts by inhibiting postoperative bone resorption in rat calvarial defects. J. Prosthodont. Res. 60, 167-76 (2016), (PMID:26868926)
- Ogata, K., Matsumura, M., Moriyama, M., Katagiri, W., Hibi, H., Nakamura, S. Cytokine Mixtures Mimicking Secretomes From Mesenchymal Stem Cells Improve Medication-Related Osteonecrosis of the Jaw in a Rat Model. JBMR Plus. 2, 69-80 (2017), (PMID:30283893)
- Ogata, K., Katagiri, W., Hibi, H. Secretomes from mesenchymal stem cells participate in the regulation of osteoclastogenesis in vitro. Clin. Oral. Investig. 21, 1979-1988 (2017), (PMID:27796573)
Product List
■Rat Osteoclast cells
| Product name | Catalog Number |
|---|---|
| Rat Osteoclasts | PMC-OSC12C |
Notes
When cells have been properly stored in liquid nitrogen and cultured according to instructions using recommended media and reagents, we guarantee against poor growth at the initiation of culture. For product support, please contact us (email: primarycell@cosmobio.co.jp). The warranty period is [within 6 months after receiving the product]. Warranty is not applicable if changes are made to the media or its usage, or if refrozen cells are utilized.