Calcified Nodule Staining kit

a convenient system for staining calcified nodule with ease

Alkaline phosphatase, osteopontin, and osteocalcin are expressed during the differentiation of bone marrow cells into mature osteoblasts and osteoblasts form calcium tubercle. Alizarin Red S, an anthraquinone derivative, has been used to identify Calcified nodule, because Calcium forms an Alizarin Red S-calcium complex, and the products turn (became) red. The Calcified Nodule Staining kit contains Substrate-containing Buffer composed mainly of Alizarin Red S and Chromogenic Substrate, and provides a convenient system for staining calcified nodule with ease.

Components

• ●Substrate-containing Buffer
• ●Chromogenic Substrate

One kit contains regents for 10 × 24-well plates

Data

Figure 1. Cells detected by Calcified Nodule Staining kit

• A) 3T3-E1 cells are cultured using Osteogenesis Culture for Osteogenesis Culture kit (Mouse) (Code No. PMC- OGCMO) (35 mm dish)
• B) Cells in Osteogenesis Culture Kit (Mouse) (PMC-OGC11) (24well plate)

Citations

1. TNX deficiency results in bone loss due to an increase in multinucleated osteoclasts.
   Biochem Biophys Res Commun. 2019 May 14;512(4):659-664. doi: 10.1016/j.bbrc.2019.03.134. Epub 2019 Mar 25. PMID: 30922562
2. Surface plasma treatment and phosphorylation enhance the biological performance of poly(ether ether ketone).
   Colloids Surf B Biointerfaces. 2019 Jan 1;173:36-42. doi: 10.1016/j.colsurfb.2018.09.032. Epub 2018 Sep 14. PMID: 30266018
3. Evaluation of Serum-Free, Xeno-Free Cryopreservation Solutions for Human Adipose-Derived Mesenchymal Stem Cells.
   Cell Med. 2016 Sep 1;9(1-2):15-20. doi: 10.3727/215517916X693122. eCollection 2017 Jan 8. PMID: 28174671
4. Ascorbic acid enhances the cold preservation period of human adipose tissue-derived mesenchymal stromal cells.
   Regen Ther. 2023 Jun 28;24:154-160. doi: 10.1016/j.reth.2023.06.008. eCollection 2023 Dec. PMID: 37868720
5. Morphological study of tooth development in podoplanin-deficient mice.
   PLoS One. 2017 Feb 21;12(2):e0171912. doi: 10.1371/journal.pone.0171912. eCollection 2017. PMID: 28222099

Product List

Calcified Nodule Staining kit

Product name	Catalog Number
Calcified Nodule Staining kit	PMC-AK21