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Calcification Evaluation Set

 

This product utilizes Alizarin Red dye solution to stain calcified nodules produced by differentiated osteoblasts, and an acid solution to extract dye bound to calcified nodules. The optical absorbance of the extracted dye solution is then measured, providing a convenient assay to evaluate the extent of osteoblast differentiation (calcification) in cultured cells.

Components

  • ●Alizarin Red Solution (Code No. CSR-ARD-A1)
  • ●Calcified Nodule Extraction Solution (Code No. CSR-ARD-E1)

One kit contains regents for 10 × 12-well plates

Data

Figure 1. Alizarin Red staining of MC3T3-E1 cells cultured in differentiation medium
Figure 2. The Effects of BMP and chondroitin sulfate E (CS-E) on the mineralization of MC3T3-E1 cells (Mean±SD)

Citations

  1. Mohawk promotes the maintenance and regeneration of the outer annulus fibrosus of intervertebral discs.
    Nat Commun. 2016 Aug 16;7:12503. doi: 10.1038/ncomms12503. PMID: 27527664

Product List

Calcification Evaluation Set

Product nameCatalog Number
Calcification Evaluation Set CSR-ARD-SET

Components

Product nameCatalog Number
Alizarin Red Solution CSR-ARD-A1
Calcified Nodule Extraction Solution CSR-ARD-E1