Calcification Evaluation Set
This product utilizes Alizarin Red dye solution to stain calcified nodules produced by differentiated osteoblasts, and an acid solution to extract dye bound to calcified nodules. The optical absorbance of the extracted dye solution is then measured, providing a convenient assay to evaluate the extent of osteoblast differentiation (calcification) in cultured cells.
Components
- ●Alizarin Red Solution (Code No. CSR-ARD-A1)
- ●Calcified Nodule Extraction Solution (Code No. CSR-ARD-E1)
One kit contains regents for 10 × 12-well plates
Data
Figure 1. Alizarin Red staining of MC3T3-E1 cells cultured in differentiation medium
Figure 2. The Effects of BMP and chondroitin sulfate E (CS-E) on the mineralization of MC3T3-E1 cells (Mean±SD)Citations
- Mohawk promotes the maintenance and regeneration of the outer annulus fibrosus of intervertebral discs.
Nat Commun. 2016 Aug 16;7:12503. doi: 10.1038/ncomms12503. PMID: 27527664
Product List
Calcification Evaluation Set
| Product name | Catalog Number |
|---|---|
| Calcification Evaluation Set | CSR-ARD-SET |
Components
| Product name | Catalog Number |
|---|---|
| Alizarin Red Solution | CSR-ARD-A1 |
| Calcified Nodule Extraction Solution | CSR-ARD-E1 |