DNA quantification kit

Simple and rapid quantification of cell number in culture system

The DNA quantification kit is a kit for compensation by comparing and quantifying the amount of DNA using fluorescence in an experimental system where cell counting is difficult.

Features

• This measurement kit is a product developed for the purpose of simply and quickly quantifying the number of cells in a culture system.
• Intracellular DNA can be easily quantified using the intercalate reaction of double-stranded DNA and the fluorescent dye Hoechst33258, and the number of cells can be standardized based on the amount of DNA.

Composition

• 10 ml coloring solution
• 2 x 150 ml buffer
• Standard solution (DNA 100 µg/ml) 2 ml x 1

Example of use

Fig. 1 Example of calibration curve prepared with standard solution

 

Frequently asked questions and answers (FAQ)

Q: Can I use buffer solutions other than those supplied with the kit?

A: RNA is also quantified in addition to DNA when buffers other than those supplied with the kit are used. Therefore, be sure to use the buffer provided with the kit (composition not disclosed).

 

References

• Kalgaonkar, S., Nishioka, H., Gross, H. B., Fujii, H. Keen, C. L., Hackman, R. M. Bioactivity of a Flavanol-rich Lychee Fruit Extract in Adipocytes and Its Effects on Oxidant Defense and Indices of Metabolic Syndrome in Animal Models. Phytother. Res. 24, 1223-1228 (2010)
• Okura, H., Komoda, H., Saga, A., Kakuta-Yamamoto, A., Hamada, Y., Fumimoto, Y., Lee, CM., Ichinose, A., Sawa, Y., Matsuyama, A. Properties of Hepatocyte-like Cell Clusters from Human Adipose Tissue-Derived Mesenchymal Stem Cells. Tissue Eng. Part C Methods. 16, 761-70 (2010)
• Saito,Y., Goto, M., Maya, K., Ogawa, N., Fujimori, K., Kurokawa, Y., Satomi, S. The Influence of Brain Death on Tissue Factor Expression in the Pancreatic Tissues and Isolated Islets in Rats. Transplant. Proc. 41, 307-310 (2009)

 

Catalog Number	Product Name	Size
PMC-AK06	DNA Quantity Kit	200 rxns