AteloCell®Cell culture ON gels
In contrast to cell-derived soluble basement membrane preparations, collagen solutions are free from bioactive compounds, nucleic acids, matrix metalloprotease (MMP), etc., allowing experimental results to be evaluated more clearly. Atelocollagen/Native collagen acidic solutions are high purity collagen solutions prepared from bovine dermis and are suitable for collagen gel fabrication. Two types of atelocollagen/native collagen products are available: I-PC (atelocollagen) and I-AC (native collagen). Atelocollagen is a derivative of collagen produced by protease treatment to remove the telopeptides at both ends of the collagen molecule.
| Product name | Cat no | Collagen | Source | Collagenase harvest | Product form | Specs | Packaging | Storage | Notes |
| Atelocollagen powder | KOU-CLP-01 | Atelo, type 1 | bovine dermis | Yes | Powder | - | 500 mg/bottle | -20°C | Highly purified atelocollagen powder with which high concentration atelocollagen solutions can be prepared for generating gel scaffolds suitable for 3D cell culture. |
| Atelocollagen, Eagle's MEM | KOU-MEN-02 | Atelo, type 1 | bovine dermis | Yes | Sterilized, neutral solution (Eagle’s MEM pH 7.4) | 2 mg/mL | 20ml/bottle | -20°C | Atelocollagen neutral solutions are high purity collagen solutions prepared from bovine dermis. They form gels under physiological conditions. These products are pre-mixed with culture medium and ready to use for cell culture. |
| Atelocollagen, DMEM Low Glucose | KOU-DME-02 | Atelo, type 1 | bovine dermis | Yes | Sterilized, neutral solution (DMEM Low Glucose pH 7.4) | 2 mg/mL | 20ml/bottle | -20°C | |
| Atelocollagen, DMEM High Glucose | KOU-DME-02H | Atelo, type 1 | bovine dermis | Yes | Sterilized, neutral solution (DMEM High Glucose pH 7.4) | 2 mg/mL | 20ml/bottle | -20°C | |
| Atelocollagen, RPMI 1640 | KOU-RPM-02 | Atelo, type 1 | bovine dermis | Yes | Sterilized, neutral solution (RPMI 1640 pH 7.4) | 2 mg/mL | 20ml/bottle | -20°C | |
| Native collagen, Bovine dermis, 3mg/mL | KOU-IAC-30 | Native, type 1 |
bovine dermis | Yes | Sterilized, acidic solution (pH 3.0) | 3 mg/mL | 50ml/bottle | 2-10°C | I-AC is an acid-soluble collagen which retains telopeptides. Its viscosity, transparency and rigidity are higher than those of I-PC. I-AC forms a gel under physiological conditions in approximately 15 min, which permits cells to remain dispersed inside the gel rather than settling to the bottom. |
| Native collagen, Bovine dermis, 5mg/mL | KOU-IAC-50 | Native, type 1 | bovine dermis | Yes | Sterilized, acidic solution (pH 3.0) | 5 mg/mL | 50ml/bottle | 2-10°C | |
| Atelocollagen, Bovine dermis, 3mg/mL | KOU-IPC-30 | Atelo, type 1 | bovine dermis | Yes | Sterilized, acidic solution (pH 3.0) | 3 mg/mL | 50ml/bottle | 2-10°C | I-PC is a protease-solubilised atelocollagen with reduced immunogenicity while maintaining collagen functions. I-PC is suitable for gel formation. I-PC takes approximately 60 min to form a gel and has low viscosity, which leads to cell settling during gel formation unless the atelocollagen concentration is kept over 0.35%. |
| Atelocollagen, Bovine dermis, 5mg/mL | KOU-IPC-50 | Atelo, type 1 | bovine dermis | Yes | Sterilized, acidic solution (pH 3.0) | 5 mg/mL | 50ml/bottle | 2-10°C |