Illustration of a mouse with a DNA helix.

Animal IVF (CARD)

Cosmo Bio USA proudly offers a premium lineup of animal in vitro fertilization (IVF) products, created in collaboration with top-tier Japanese research institutions including the Center for Animal Resources and Development (CARD) at Kumamoto University and the Collaborative System for Reproductive Biotechnology (CSR). These trusted solutions are designed to dramatically boost reproductive success in key research models like mice, rats, and pigs. For mice, the powerful CARD IVF System features FERTIUP® Cryoprotectant for reliable sperm freezing, Preincubation Medium (PM) to enhance motility and fertilization, and CARD MEDIUM®, which transforms fertilization efficiency—even in tough strains like C57BL/6 and 129—by increasing success rates from under 10% to over 80%. HyperOva® and its freeze-dried form, HyperOva FD, further accelerate workflows by delivering significantly more oocytes per female for higher embryo yields.

Expanding beyond mice, Cosmo Bio’s rat IVF solutions include CARD FERTIUP® Rat Sperm Cryoprotectant and CARD Rat IVF Medium, engineered for high-performance sperm preservation and fertilization. For swine models, CSR’s Pig Fertilization Medium and Pig Oocyte Maturation Medium enable consistent and efficient oocyte maturation and IVF. All CARD and CSR reagents are produced under ISO9001 quality standards, packaged in secure glass ampoules, and stored at 4°C for maximum stability and performance. Whether you're advancing genetic engineering, developing transgenic lines, or driving cutting-edge biomedical research, Cosmo Bio USA’s IVF systems provide the reliability, innovation, and results today’s scientists demand.

 

  • Rat KSOM (Potassium Simplex Optimized Medium)

    Cosmo Bio

    Rat KSOM (Potassium Simplex Optimized Medium)

    Catalog No.(s): CSR-R-R151

    See all IVF and fertility-related products available from Cosmo Bio USA Purpose of Use For rat IVF procedures Produced by strict quality control based on ISO9001 (Global quality management standard) Developer Dr. Kazoumi Nakamura (Tottori...

    $585.00
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  • CARD HyperOva for RAT (superovulation reagent)

    Cosmo Bio

    CARD HyperOva for RAT (superovulation reagent)

    Catalog No.(s): KYD-FR-003, KYD-FR-003-X5

    CARD HyperOva for RAT (Cat No. KYD-FR-003) is a superovulation reagent that induces ovulation at higher efficiency when compared with superovulation with eCG/hCG. Contributues to the reduction of 3Rs! (Replacement, Reduction, Refinement) More oocytes...

    $222.00 - $1,030.00
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  • Cold storage solution for cauda epididymides

    Cosmo Bio

    Cold storage solution for cauda epididymides

    Catalog No.(s): KYD-007-EX, KYD-007-EX-X5, KYD-007-EX-X10

    Storage of cauda epididymides [Note: We recommend usage with our "Card Cold Transport Kit (Cat. No. KYD-006-EX)". Storage: -20C * For short time storage, it is stable for 3 months after storing at 4C. Avoid freeze/thaw cycles. Expiration...

    $134.00 - $1,284.00
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  • CP-1(R) High Grade

    Cosmo Bio

    CP-1(R) High Grade

    Catalog No.(s): KYO-27207, KYO-27206

    For cryopreservation of hematopoieticstem cells, dimethyl sulfoxide (DMSO) has generally been used as a cryoprotectant. In the case ofcryopreservation with DMSO alone, it was common to be stored in liquid nitrogen...

    $187.00 - $307.00
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  • CARD Rat IVF Medium

    Cosmo Bio

    CARD Rat IVF Medium

    Catalog No.(s): KYD-FR-002, KYD-FR-002-X5, KYD-FR-002-X10

    Save BIG (5-7%) on multipack and multipack bundles!...

    $44.00 - $412.00
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  • CARD Rat Freezing Kit

    Cosmo Bio

    CARD Rat Freezing Kit

    Catalog No.(s): KYD-GAR-001

    RAT SPERM FREEZING KIT Available Sizes / Cat. CARD Rat Sperm Freezing Kit is hardware accessory that facilitates the production of high-quality frozen rat sperm during cryopreservation with CARD FERTIUP® Rat Sperm...

    $198.00
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  • SPB1

    Cosmo Bio

    SPB1

    Catalog No.(s): CSR-R-S187, CSR-R-S188

    See all IVF and fertility-related products available from Cosmo Bio USA Usage For thawing rat cryopreserved embryos. Storage Keep them in 4℃ until use. Use all the media once opened and avoid using the remaining because of the quality degradation...

    $351.00 - $702.00
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  • CARD HyperOva FD

    Cosmo Bio

    CARD HyperOva FD

    Catalog No.(s): KYD-015-EX, KYD-015-EX-X5, KYD-015-EX-X25, KYD-015-EX-X50

    Put more eggs in your basket! CARD HyperOva® (Japanese Patent No. 5927588) is an ultra-superovulation reagent that induces ovulation at higher efficiency when compared with the conventional superovulation induction method (PMSG-hCG method)...

    $196.00 - $8,399.00
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  • CARD 0.25M Sucrose

    Cosmo Bio

    CARD 0.25M Sucrose

    Catalog No.(s): KYD-014-EX-2, KYD-014-EX-5

    Documents & Links for CARD 0.25M Sucrose Manual KYD Catalog (2023 ver) Documents & Links for CARD 0.25M Sucrose Manual KYD Catalog (2023 ver)

    $30.00 - $59.00
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  • CARD DAP213

    Cosmo Bio

    CARD DAP213

    Catalog No.(s): KYD-013-EX-0.5, KYD-013-EX-1

    Documents & Links for CARD DAP213 Manual KYD Catalog (2023 ver) Documents & Links for CARD DAP213 Manual KYD Catalog (2023 ver)

    $15.00 - $30.00
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  • CARD 1M DMSO

    Cosmo Bio

    CARD 1M DMSO

    Catalog No.(s): KYD-012-EX

    Documents & Links for CARD 1M DMSO Flyer CARD 1M DMSO Flyer Manual KYD Catalog (2023 ver) Documents & Links for CARD 1M DMSO Flyer CARD 1M DMSO Flyer Manual KYD Catalog (2023 ver)

    $15.00
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  • CARD KSOM

    Cosmo Bio

    CARD KSOM

    Catalog No.(s): KYD-011-EX-2, KYD-011-EX-5

    Documents & Links for CARD KSOM Manual KYD Catalog (2023 ver) Documents & Links for CARD KSOM Manual KYD Catalog (2023 ver)

    $30.00 - $59.00
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  • R18S3 (Medium for Mouse Sperm Cryopreservation)

    Cosmo Bio

    R18S3 (Medium for Mouse Sperm Cryopreservation)

    Catalog No.(s): CSR-R-R082

    See all IVF and fertility-related products available from Cosmo Bio USA Procedures - Freezing Pick up the cauda epididymis from male C57BL/6J mouse, remove adherent adipose tissue and blood, and put two cauda epididymis into 100 µL of R18S3 on...

    $264.00
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  • Reproductive Engineering Techniques in Mice Technical Manual

    Cosmo Bio

    Reproductive Engineering Techniques in Mice Technical Manual

    Catalog No.(s): KYD-MANUAL

    Written by Dr. Naomi Nakagata, this beautifully bound, full color manual offers step-by-step protocols with illustrative pictures, media composition, and references. It is the perfect companion piece to accompany your mouse IVF work performed with...

    $0.00
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  • Glass capillary 20PC

    Cosmo Bio

    Glass capillary 20PC

    Catalog No.(s): KYD-S037

    Documents & Links for Glass capillary 20PC Manual KYD Catalog (2023 ver) Documents & Links for Glass capillary 20PC Manual KYD Catalog (2023 ver)

    $88.00
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  • Embryo manipulation instrument set

    Cosmo Bio

    Embryo manipulation instrument set

    Catalog No.(s): KYD-S036

    Documents & Links for Embryo manipulation instrument set Manual KYD Catalog (2023 ver) Documents & Links for Embryo manipulation instrument set Manual KYD Catalog (2023 ver)

    $101.00
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  • Triangular Cassette Long (10units)

    Cosmo Bio

    Triangular Cassette Long (10units)

    Catalog No.(s): KYD-S035

    Documents & Links for Triangular Cassette Long (10units) Manual KYD Catalog (2023 ver) Documents & Links for Triangular Cassette Long (10units) Manual KYD Catalog (2023 ver)

    $192.00
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  • Straw Connector

    Cosmo Bio

    Straw Connector

    Catalog No.(s): KYD-S025

    Documents & Links for Straw Connector Manual KYD Catalog (2023 ver) Documents & Links for Straw Connector Manual KYD Catalog (2023 ver)

    $23.00
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  • Triangular Cassette Short (10units)

    Cosmo Bio

    Triangular Cassette Short (10units)

    Catalog No.(s): KYD-S021

    Documents & Links for Triangular Cassette Short (10units) Manual KYD Catalog (2023 ver) Documents & Links for Triangular Cassette Short (10units) Manual KYD Catalog (2023 ver)

    $142.00
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  • Sperm Straws (10 Pieces x 10 Units)

    Cosmo Bio

    Sperm Straws (10 Pieces x 10 Units)

    Catalog No.(s): KYD-S020X10

    Documents & Links for Sperm Straws (10 Pieces x 10 Units) Manual KYD Catalog (2023 ver) Documents & Links for Sperm Straws (10 Pieces x 10 Units) Manual KYD Catalog (2023 ver)

    $150.00
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  • Freezing Canister

    Cosmo Bio

    Freezing Canister

    Catalog No.(s): KYD-S018

    Documents & Links for Freezing Canister Manual KYD Catalog (2023 ver) Documents & Links for Freezing Canister Manual KYD Catalog (2023 ver)

    $87.00
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  • CARD HyperOva (Superovulation Reagent for mouse)

    Cosmo Bio

    CARD HyperOva (Superovulation Reagent for mouse)

    Catalog No.(s): KYD-010-EX, KYD-010-EX-X5, KYD-010-06-EX, KYD-010-06-EX-X5, KYD-010-06-EX-X25, KYD-010-06-EX-X50, KYD-010-EX-X25, KYD-010-EX-X50

    Save BIG (up to 11%) on multipacks and multipack bundles!...

    CARD HyperOva® (Superovulation Reagent for mouse) is an ultra-superovulation reagent that is 3-4 times more efficient at inducing ovulation than the conventional PMSG-hCG method (80 ova per C57BL/6 mouse versus approximately 20)....

    A mixture of equine chorionic gonadotropin and goat anti-inhibin antiserum, CARD HyperOva® is also effective with many other strains....

    $67.00 - $4,191.00
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  • Cosmo Bio

    CARD mHTF (modified Human Tubal Fluid)

    Catalog No.(s): KYD-008-02-EX, KYD-008-02-EX-X5, KYD-008-05-EX, KYD-008-05-EX-X3, KYD-008-02-EX-X25, KYD-008-02-EX-X50, KYD-008-05-EX-X15, KYD-008-05-EX-X30

    CARD mHTF (modified Human Tubal Fluid) is an essential workhorse reagent used in a wide variety of mouse in vitro fertilization procedures....

    Save BIG (up to 26%) on multipack and multipack bundles!...

    $44.00 - $2,513.00
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  • CARD Cold Transport Kit

    Cosmo Bio

    CARD Cold Transport Kit

    Catalog No.(s): KYD-006-EX

    Safe transport of mouse cauda epididymides and embryos at cold temperatures The CARD Cold Transport Kit is a kit specially-designed for the cheap, safe transport of mouse cauda epididymides and embryos at cold temperatures. The CARD Cold Transport Kit...

    $192.00
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  • CARD FERTIUP PM 0.5 mL - CARD MEDIUM Set

    Cosmo Bio

    CARD FERTIUP PM 0.5 mL - CARD MEDIUM Set

    Catalog No.(s): KYD-005-EX, KYD-005-EX-X5, KYD-005-EX-X10

    Save BIG (5-7%) on multipacks!...

    This convenient double medium set comprises CARD FERTIUP® PM and CARD MEDIUM® that when used together synergize to elevate fertilization rates in mouse IVF. ...

    CARD FERTIUP® PM is a sperm fertility-enhancing preculture medium. CARD MEDIUM® is a novel fertilization-promoting culture medium that acts during IVF on the mouse egg zona pellucida. ...

    $216.00 - $2,027.00
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  • CARD FERTIUP PM 1 mL - CARD MEDIUM Set

    Cosmo Bio

    CARD FERTIUP PM 1 mL - CARD MEDIUM Set

    Catalog No.(s): KYD-004-EX, KYD-004-EX-X5, KYD-004-EX-X10

    Save BIG (5-7%) on multipacks!...

    This convenient double medium set comprises CARD FERTIUP® PM and CARD MEDIUM® that when used together synergize to elevate fertilization rates in mouse IVF. ...

    CARD FERTIUP® PM is a sperm fertility-enhancing preculture medium. CARD MEDIUM® is a novel fertilization-promoting culture medium that acts during IVF on the mouse egg zona pellucida....

    $254.00 - $2,384.00
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  • CARD MEDIUM

    Cosmo Bio

    CARD MEDIUM

    Catalog No.(s): KYD-003-EX, KYD-003-EX-X5, KYD-003-EX-X10

    Save BIG (5-7%) on multipacks!...

    CARD MEDIUM® is a novel culture medium that acts during IVF on the mouse egg zona pellucida to promote high fertilization rates, especially when used in conjunction with CARD FERTIUP® Preincubation Medium: PM. ...

    CARD MEDIUM® was developed by The Center for Animal Resources and Development (CARD), Kumamoto University, Japan....

    $187.00 - $1,752.00
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  • CARD FERTIUP Preincubation Medium: PM

    Cosmo Bio

    CARD FERTIUP Preincubation Medium: PM

    Catalog No.(s): KYD-002-EX, KYD-002-EX-X5, KYD-002-05-EX-X5, KYD-002-05-EX, KYD-002-05-EX-X25, KYD-002-05-EX-X50, KYD-002-EX-X25, KYD-002-EX-X50

    Save BIG (8-15%) on multipack and multipack bundles!...

    CARD FERTIUP® Preincubation Medium: PM is a preculture medium that enhances mouse sperm fertility. ...

    Used in IVF with thawed C57BL/6 mouse sperm frozen using CARD FERTIUP® Cryoprotectant: CPA, fertilization rates of 30% or more can be expected. CARD FERTIUP® Preincubation Medium: PM also effectively promotes IVF with fresh sperm....

    $59.00 - $4,392.00
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  • CARD FERTIUP Cryoprotectant: CPA

    Cosmo Bio

    CARD FERTIUP Cryoprotectant: CPA

    Catalog No.(s): KYD-001-EX, KYD-001-EX-X5, KYD-001-05-EX-X5, KYD-001-05-EX, KYD-001-05-EX-X25, KYD-001-05-EX-X50, KYD-001-EX-X25, KYD-001-EX-X50

    Save BIG (8-15%) on multipack and multipack bundles!...

    CARD FERTIUP® Cryoprotectant: CPA is a mouse sperm cryoprotectant that guards against damage to spermatozoa during cryopreservation. ...

    This reagent significantly improves in vitro fertilization rates of frozen and thawed sperm even with notoriously IVF-inefficient mouse strains such as C57BL/6 and 129. Use it to preserve and protect your valuable mouse resources....

    $59.00 - $4,392.00
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  • Sucrose 0.25M

    Cosmo Bio

    Sucrose 0.25M

    Catalog No.(s): CSR-R-Y077, CSR-R-Y078

    Sucrose 0.25M, a Cosmo Bio brand product, can be used interchangeably with CARD 0.25M Sucrose (KYD-014-EX-2) for mouse IVF procedures. ...

    Produced and packaged in Japan under ISO9001 global quality management standards, it contains NaCl, KCl, CaCl2, KH2PO4, MgCl2.6H2O, Na2HPO4, Na-Pyruvate, Glucose, Penicillin, Streptomycin, BSA, Sucrose....

    $235.00 - $468.00
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  • DAP213 (DMSO, Acetamide, Propylene Glycol)

    Cosmo Bio

    DAP213 (DMSO, Acetamide, Propylene Glycol)

    Catalog No.(s): CSR-R-T073

    DAP213 (DMSO, Acetamide, Propylene Glycol), a Cosmo Bio brand product, can be used interchangeably with CARD DAP213 (KYD-013-EX-0.5) for mouse IVF procedures. ...

    Produced and packaged in Japan under ISO9001 global quality management standards, it contains NaCl, KCl, CaCl2, KH2PO4, MgCl2E6H2O, Na2HPO4, Na-Pyruvate, glucose, penicillin, streptomycin, BSA, DMSO, propylene glycol and acetamide....

    $235.00
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  • DMSO 1M

    Cosmo Bio

    DMSO 1M

    Catalog No.(s): CSR-R-T072

    DMSO 1M (Cosmo Bio brand) can be used interchangeably with CARD 1M DMSO (KYD-012-EX) for mouse IVF procedures. ...

    Produced and packaged in Japan under ISO9001 global quality management standards, it contains DMSO, NaCl, KCl, CaCl2, KH2PO4, MgCl26H2O, Na2HPO4, Na-pyruvate, glucose, penicillin, streptomycin and BSA....

    $235.00
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  • PEPeS (Percoll in PB1)

    Cosmo Bio

    PEPeS (Percoll in PB1)

    Catalog No.(s): CSR-R-P187

    See all IVF and fertility-related products available from Cosmo Bio USA Purpose of Use For embryo freezing Produced by strict quality control based on ISO9001 (Global quality management standard) Expiry date 1 year from production Delivery...

    $464.00
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