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CosmoSonic™ II Ultrasonic Disruptor
Closed-tube, multi-sample, temperature-controlled, ultrasonicator manufactured for Cosmo Bio in Japan by Sonic Bio (formerly Tosho Denki), the company that set the standard in multiplex sonication for DNA shearing more than twenty years ago. With compatible tube-holder accessories, CosmoSonic II can process up to 48 samples in a single run.
Background
For over twenty years, water-bath type ultrasonic disruptors manufactured by Sonic Bio in Japan and sold by Cosmo Bio has been the instrument of choice for hundreds of labs around the world needing to shear DNA into fragments suitable for chromatin immunoprecipitation (ChIP) and other applications. CosmoSonic™ II Ultrasonic Cell Disruptor is a customizable unit that uses indirect ultrasonic energy to shear DNA or disrupt cells.
How CosmoSonic Ultrasonicators Work
Ultrasonic Generator - Sample in capped tubes are held at a fixed distance below the water surface in a sonication tank and rotated through the water to ensure even exposure of samples an applied ultrasonic energy field emanating from a generator below the tank. In a process known as cavitation, rapidly collapsing bubbles generated by ultrasonic waves provide the force responsible for DNA shearing or cell lysis.
Larger tubes (10, 15, and 50 ml) are held in place by a toothed gear plate alone (gear plate/tube holder). The teeth of a gear plate/tube holder engage a matching gear fixed to a small electric motor, rotating the samples through the sonication tank. Smaller tubes (0.5 mL and 1.5 ml) require an adaptor unit that screws on to gear plate. Note that for larger sample volumes, the efficiency and reproducibility of sonication greatly increased by placing a metal resonance rod in the sample tube. To best fit your labs workflow, a variety of gear plates, tube adaptors, and resonance rod units are available.
"Smart" Iced Water Circulator - CosmoSonic ultrasonicators utilize a separate iced-water circulator unit to dissipate detrimental heat generated by cavitation. Note that this unit is not a refrigerator. Water and crushed ice need to be mixed in the circulator tank. To allow sufficient time for heat dissipation and to prevent water circulation from disturbing the ultrasonic wave field, the circulator is only active when the ultrasonic wave generator is in the OFF state during a programed run cycle.
CosmoSonic Applications
- Fragmentation of chromatin at ChIP Assay
- Fragmentation of genome DNA for next-generation sequencer
- Extraction of nucleic acid, protein, pigment etc. from cell/tissue
- Disruption, etc.
- Dissolution of hard-soluble samples
- Dispersion of nano particle or catalyser for fuel battery
Why choose CosmoSonic™ II?
- Gentle: Because it uses a gentle method of sonication, the integrity of DNA is retained. It is gentler than other sonicators, and thus, produces better and more consistent results than with harsher sonication methods.
- Efficient: The gear plates and silver tube attachments permit many samples to be sonicated in parallel.
- Even Distribution of Energy: Continuous rotation of tubes allows even distribution of the energy for efficient sonication, which promotes reproducible results for a small or large number of samples.
- Customizable: The CosmoSonic™ II can process samples ranging in volume from 0.5 mL to 50 mL depending on the accessory pieces chosen.
- High Reproducibility: Because the CosmoSonic™ II enables automation of sonication, there is higher reproducibility of results when compared to manual systems.
- Clean: The CosmoSonic™ II is a hermetically sealed unit that can be tested free of biohazards, contaminations, and radioisotopes.
- Easy to Operate: An integrated programmable timer allows for easy setup.-
- Quiet: The included high-level Sound Absorbing Box reduces noise from ultrasonic sources.
Fundamentally, all base unit Types are similar, differing only in the size of the gear plate/tube holder and the number of gear plates accepted.
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Gear Plates/Tube Holders and Microtube Tube Adaptors |
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Without adaptor |
With adaptor MAT-05 |
With adaptor MAT-15 |
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Holds 6 x 10 ml tubes or 6 x 15 ml tubes |
Holds 12 x 0.5 ml tubes |
Holds 6 x 1.5 ml tubes |
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Holds 3 x5 0 ml tubes |
Not compatible |
Not compatible |
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Gear Plates/Tube Holders and MicroTube Adaptors |
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Without adaptor |
With adaptor MAT-310-05 |
With adaptor MAT-310-15 |
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Holds 12 x 10 ml tubes or 12 x 15 ml tubes |
Holds 24 x 0.5 ml tubes |
Holds 12 x 1.5 ml tubes |
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Holds 6 x 50ml tubes |
Holds 24 x 0.5 ml tubes |
Holds 12 x 1.5 ml tubes |
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*Type 12 accepts one compatible gear plate. |
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| Type 6 | Type 12 | Type 24 |
| recommended volume | adaptors needed to process indicated max tubes | ||||
| tube size | min/max | Max tubes held | Compatible Gear Plates | Mircrotube Holder (needed for 0.5 and 1.5 ml tubes only) | Resonance Rod |
| 0.5 ml | 50/100 | 12 | NG-6  |
MAT-05  |
none |
| 1.5 ml | 100/300 | 6 | NG-6 | MAT-15 | none |
| 10 ml AS-500 | 0.5/2 | 6 | NG-6 | none | CHIP-10 |
| 15 ml | 0.5/2 | 6 | |||
| Falcon or Greiner | NG-6 | none | FT-15-WS | ||
| Corning or Iwaki | NG-6 | none | CT-15-WS | ||
| Smilon | NG-6 | none | ST-15-WS | ||
| TPP | NG-6 | none | TT-15WS | ||
| 50 ml | 3/20 | 3 | |||
| Falcon | NG-50-3 | none | FT-50WS | ||
| Griener | NG-50-3 | none | FT-50WS | ||
| Nalgene | NG-50-3 | none | |||
| Corning | NG-50-3 | none | CT-50WS | ||
| Iwaki | NG-50-3 | none | CT-50WS | ||
| Smilon | NG-50-3 | none | ST-50WS | ||
| recommended working volume | maximum number of tubes possible per model | ||||
| tube size | minimum | maximum | Type 6 | Type 12 | Type 24 |
| 0.5 ml | 50 | 100 | 12 | 24 | 48 |
| 1.5 ml | 100 | 300 | 12 | 24 | 48 |
| 10 ml | 0.5 | 2 | 6 | 12 | 24 |
| 15 ml | 0.5 | 2 | 6 | 12 | 24 |
| 50 ml | 3 | 20 | 3 | 6 | 12 |
Maintain consistent sample cooling with the Ice Cold Water Circulator (sold separately) (shown below by itself (left) and with a Type 12 (right))
Accessories
Choose from a variety of accessories to accommodate your wave disruption needs (number/size of samples)
