Development

  • Example Assay Data: 1. Sample Preparation. Inserted c-Myc tag to C-terminus end of DasherGFP plasmid vector (DNA2.0) Protein was expressed in E.coli and lysed (Crude sample). Crude sample as separated by SDS-PAGE. Part of the fraction was used for CBB Staining, rest for Western Blotting. 2. Immunoblotting analysis. Primary Antibody (anti c-Myc [CAC-XIM-MA001]) 1 : 1000 dilution (1μg/mL) Secondary Antibody (anti mouse IgG-HRP, Bethyl Laboratories, Cat.No. A90-216P) 1 : 3000 dilution Anti c-Myc [CAC-XIM-MA001] detection of c-Myc tag: Based on CBB Staining result, the c-Myc tagged fluorescent protein exhibited a higher molecular weight compared to the non-tagged version of fluorescent protein. Further, the Anti c-Myc antibody detected the c-Myc tagged- but not the non-tagged version of fluorescent protein.

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    Anti Myc Proto-Oncogene Protein (c-Myc) mAb (Clone 9E10)

    Catalog No.(s): CAC-XIM-MA001

    Catalog numbers beginning with "CAC" are antibodies from our exclusive Cosmo Bio Antibody Collection. Visit the CAC Antibody homepage to browse the collection list, organized by research topic.c-Myc is a very potent oncogene upregulated in...

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  • Figure 1. Immuno blot analysis for 6 kinds of tau isoforms (3-repeat and 4-repeat) with anti total tau (T46) and Anti 4R (TIP-4RT-P01).

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    Anti-4R-Tau pAb (Rabbit, Antiserum)

    Catalog No.(s): CAC-TIP-4RT-P01

    Anti-4R-Tau pAb — prepared from rabbits immunized with synthetic N279D 4R-tau peptide [VQIIDKKLDLSNVQSKC] — recognizes 4R tau isoforms, regardless of deamidation as occurs extensively in AD brains....

    Validated for western blot and IHC(p), this isoform-specific tau antibody is useful for immunohistochemical and biochemical studies of tau species in diverse neurodegenerative diseases....

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  • Immunoblot analysis. Rib cartilage extract (2.5 μg protein) from 4-week-old rat was subjected to immunoblot analysis using bChM-10 antibody at 500-fold dilution. ChM-1 is detected as a diffuse band of 20-25 kDa due to variation in glycosylation. This antibody also detects the N-terminal glycosylation domain-deleted form of ChM-1(14 kDa ChM-1).

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    Anti Chondromodulin-I (ChM-I) mAb (Clone bCHM-10)

    Catalog No.(s): CAC-TCS-010

    Catalog numbers beginning with "CAC" are antibodies from our exclusive Cosmo Bio Antibody Collection. Visit the CAC Antibody homepage to browse the collection list, organized by research topic.Click here to browse a well organized list of...

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  • Immunoblot analysis. Rib cartilage extract (2.5 μg protein) from 4-week-old rat was subjected to immunoblot analysis using bChM-9 antibody at 500-fold dilution. ChM-1 is detected as a diffuse band of 20-25 kDa due to variation in glycosylation. This antibody also detects the N-terminal glycosylation domain-deleted form of ChM-1(14 kDa ChM-1).

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    Anti Chondromodulin-I (ChM-I) mAb (Clone bCHM-9)

    Catalog No.(s): CAC-TCS-009

    Catalog numbers beginning with "CAC" are antibodies from our exclusive Cosmo Bio Antibody Collection. Visit the CAC Antibody homepage to browse the collection list, organized by research topic.Click here to browse a well organized list of...

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  • Immunoblot analysis. Rib cartilage extract (2.5 μg protein) from 4-week-old rat was subjected to immunoblot analysis using bChM-8 antibody at 500-fold dilution. ChM-1 is detected as a diffuse band of 20-25 kDa due to variation in glycosylation. This antibody also detects the N-terminal glycosylation domain-deleted form of ChM-1(14 kDa ChM-1).

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    Anti Chondromodulin-I (ChM-I) mAb (Clone bCHM-8)

    Catalog No.(s): CAC-TCS-008

    Catalog numbers beginning with "CAC" are antibodies from our exclusive Cosmo Bio Antibody Collection. Visit the CAC Antibody homepage to browse the collection list, organized by research topic.Click here to browse a well organized list of...

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  • Immunoblot analysis. Rib cartilage extract (2.5 μg protein) from 4-week-old rat was subjected to immunoblot analysis using bChM-7 antibody at 500-fold dilution. ChM-1 is detected as a diffuse band of 20-25 kDa due to variation in glycosylation. This antibody also detects the N-terminal glycosylation domain-deleted form of ChM-1(14 kDa ChM-1).

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    Anti Chondromodulin-I (ChM-I) mAb (Clone bCHM-7)

    Catalog No.(s): CAC-TCS-007

    Catalog numbers beginning with "CAC" are antibodies from our exclusive Cosmo Bio Antibody Collection. Visit the CAC Antibody homepage to browse the collection list, organized by research topic.Click here to browse a well organized list of...

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  • Immunoblot analysis. Rib cartilage extract (2.5 μg protein) from 4-week-old rat was subjected to immunoblot analysis using bChM-6 antibody at 500-fold dilution. ChM-1 is detected as a diffuse band of 20-25 kDa due to variation in glycosylation. This antibody also detects the N-terminal glycosylation domain-deleted form of ChM-1(14 kDa ChM-1).

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    Anti Chondromodulin-I (ChM-I) mAb (Clone bCHM-6)

    Catalog No.(s): CAC-TCS-006

    Catalog numbers beginning with "CAC" are antibodies from our exclusive Cosmo Bio Antibody Collection. Visit the CAC Antibody homepage to browse the collection list, organized by research topic.Click here to browse a well organized list of...

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  • Immunostaining of mouse cartilage.

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    Anti Chondromodulin-I (ChM-I) mAb (Clone hCHM-5)

    Catalog No.(s): CAC-TCS-005

    Catalog numbers beginning with "CAC" are antibodies from our exclusive Cosmo Bio Antibody Collection. Visit the CAC Antibody homepage to browse the collection list, organized by research topic.Click here to browse a well organized list of...

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  • Recombinant human ChM-1 (5 ng) was subjected to immunoblot analysis using hChM-3 antibody at 1/500 dilution.

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    Anti Chondromodulin-I (ChM-I) mAb (Clone hCHM-4)

    Catalog No.(s): CAC-TCS-004

    Catalog numbers beginning with "CAC" are antibodies from our exclusive Cosmo Bio Antibody Collection. Visit the CAC Antibody homepage to browse the collection list, organized by research topic.Click here to browse a well organized list of...

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  • Anti Chondromodulin-I (ChM-I) mAb (Clone hCHM-3)

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    Anti Chondromodulin-I (ChM-I) mAb (Clone hCHM-3)

    Catalog No.(s): CAC-TCS-003

    Catalog numbers beginning with "CAC" are antibodies from our exclusive Cosmo Bio Antibody Collection. Visit the CAC Antibody homepage to browse the collection list, organized by research topic.Click here to browse a well organized list of...

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  • Recombinant human ChM-1 (5 ng) was subjected to immunoblot analysis using hChM-2 antibody at 1/100 dilution.

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    Anti Chondromodulin-I (ChM-I) mAb (Clone hCHM-2)

    Catalog No.(s): CAC-TCS-002

    Catalog numbers beginning with "CAC" are antibodies from our exclusive Cosmo Bio Antibody Collection. Visit the CAC Antibody homepage to browse the collection list, organized by research topic.Click here to browse a well organized list of...

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  • Anti Chondromodulin-I (ChM-I) mAb (Clone hCHM-1)

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    Anti Chondromodulin-I (ChM-I) mAb (Clone hCHM-1)

    Catalog No.(s): CAC-TCS-001

    Catalog numbers beginning with "CAC" are antibodies from our exclusive Cosmo Bio Antibody Collection. Visit the CAC Antibody homepage to browse the collection list, organized by research topic.Click here to browse a well organized list of...

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  • Anti Filamin-A (FLN-A) pAb (Rabbit, Antiserum)

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    Anti Filamin-A (FLN-A) pAb (Rabbit, Antiserum)

    Catalog No.(s): CAC-SU-IZ-P05

    Catalog numbers beginning with "CAC" are antibodies from our exclusive Cosmo Bio Antibody Collection. Visit the CAC Antibody homepage to browse the collection list, organized by research topic.Click here to browse a well organized list of...

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  • Figure 1. Western blotting of purified human aorta versican (primarily a mixture of V1 and V2 isoforms) resolved by SDS-PAGE on 3-8% linear gradient gels under reducing conditions (MW, HiMark Unstained Protein Standard) before (Intact) and after combined chondroitinase ABC and endo-b-galactosidase pretreatment (Digested). * Banding pattern observed by immunoblotting depends upon the isoforms and is often complex. In the intact forms, i.e. without removal of GAGs, V0, V1 and V2 do not enter acrylamide gels and therefore agarose gels are recommended. Following chondroitinase-digestion and extensive enzymatic deglycosylation, most isoforms still show complex, smeared banding patterns.

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    Anti Versican (VCAN) mAb (Clone 6B10)

    Catalog No.(s): CAC-PRPG-VS-M04

    Catalog numbers beginning with "CAC" are antibodies from our exclusive Cosmo Bio Antibody Collection. Visit the CAC Antibody homepage to browse the collection list, organized by research topic.Click here to browse a well organized list of...

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  • Figure 1. Western blotting of purified human aorta versican resolved by SDS-PAGE on 3-8% linear gradient gels (MW, HiMark Unstained Protein Standard) before (Intact) and after combined chondroitinase ABC and endo-b-galactosidase pretreatment (Digested). * Banding pattern observed by immunoblotting depends upon the isoforms and is often complex. In the intact forms, i.e. without removal of GAGs, V0, V1 and V2 do not enter acrylamide gels and therefore agarose gels are recommended. Following chondroitinase-digestion and extensive enzymatic deglycosylation, most isoforms still show complex, smeared banding patterns.

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    Anti Versican (VCAN) mAb (Clone 2B3)

    Catalog No.(s): CAC-PRPG-VS-M03

    Catalog numbers beginning with "CAC" are antibodies from our exclusive Cosmo Bio Antibody Collection. Visit the CAC Antibody homepage to browse the collection list, organized by research topic.Click here to browse a well organized list of...

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  • Figure 1. Immunoblotting of intact versican (mixture of V1 and V2 isoforms) prior to (Intact) and after combined chondroitinase ABC (Chase ABC) and endo-galactosidase-digestion (Digested). The proteoglycan was resolved by SDS-PAGE under reducing conditions on 3-8% linear gradient gels (MW, HiMark Unstained Protein Standard). Banding pattern depends upon the isoforms and is often complex. In the intact forms, i.e. without removal of GAGs, isoforms V0, V1 and V2 do not enter conventional polyacrylamide gels and therefore alternative gel types are strongly recommended. Following chondroitinase-digestion and extensive enzymatic deglycosylation, most isoforms still show complex, smeared banding patterns.

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    Anti Versican/CSPG2 (VCAN) mAb (Clone 4C5)

    Catalog No.(s): CAC-PRPG-VS-M02

    Catalog numbers beginning with "CAC" are antibodies from our exclusive Cosmo Bio Antibody Collection. Visit the CAC Antibody homepage to browse the collection list, organized by research topic.Click here to browse a well organized list of...

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  • Figure 1. Immunoblotting of intact versican (mixture of V1 and V2 isoforms) in its untreated and chondroitinase ABC (Chase ABC)-digested form, or after combined digestion with chondroitinase ABC and a number of exo- and endoglycosydases. The proteoglycan was resolved by SDS-PAGE under reducing conditions on 3-8% linear gradient gels (MW, HiMark Unstained Protein Standard).

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    Anti Versican/CSPG2 (VCAN) mAb (Clone 5C12)

    Catalog No.(s): CAC-PRPG-VS-M01

    Catalog numbers beginning with "CAC" are antibodies from our exclusive Cosmo Bio Antibody Collection. Visit the CAC Antibody homepage to browse the collection list, organized by research topic.Click here to browse a well organized list of...

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  • Immunostaining of malignant glioblastoma A) and cutaneous melanoma C) lesions. B) Western blot of whole tissue/cell lysates of a human glioblastoma lesion and the human melanoma A375 cell line after SDS-PAGE under reducing conditions on 4-10% gradient gels. D) Immunostaining of human melanoma MeWo cells. Nuclear counterstaining was performed with Hoescht.

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    Anti NG2 (CSPG4) mAb (Clone 2161D7)

    Catalog No.(s): CAC-PRPG-NG-M01

    Catalog numbers beginning with "CAC" are antibodies from our exclusive Cosmo Bio Antibody Collection. Visit the CAC Antibody homepage to browse the collection list, organized by research topic.Click here to browse a well organized list of...

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  • Figure 1. Immunoprecipitation of biotinylated laminin-nidogen complexes resolved by SDS-PAGE on 5% gels under reducing conditions and visualized by chemiluminescence; red arrow points to the nidogen band, whereas upper bands correspond to a, b and g laminin chains.

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    Anti Laminin/Nidogen complex mAb (Clone 331G3)

    Catalog No.(s): CAC-PRPG-NDG-M01

    Catalog numbers beginning with "CAC" are antibodies from our exclusive Cosmo Bio Antibody Collection. Visit the CAC Antibody homepage to browse the collection list, organized by research topic.Click here to browse a well organized list of...

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  • Anti Laminin Alpha-4 mAb (Clone 652C4)

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    Anti Laminin Alpha-4 mAb (Clone 652C4)

    Catalog No.(s): CAC-PRPG-LA4-M01

    Catalog numbers beginning with "CAC" are antibodies from our exclusive Cosmo Bio Antibody Collection. Visit the CAC Antibody homepage to browse the collection list, organized by research topic.Click here to browse a well organized list of...

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  • Immunolabelling of human adult skin. B) Immunoblot analysis of sarcoma HT1080 whole-cell extracts; C) Flow cytometric analysis of A375 melanoma cells.

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    Anti Integrin Alpha-6 (VLA-6) mAb (Clone 537D5)

    Catalog No.(s): CAC-PRPG-ITG-M01

    Catalog numbers beginning with "CAC" are antibodies from our exclusive Cosmo Bio Antibody Collection. Visit the CAC Antibody homepage to browse the collection list, organized by research topic.Click here to browse a well organized list of...

    $576.00
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  • Figure 1. Western blotting on intact (left) and chondroitinase ABC-digested (right) bovine decorin after SDS-PAGE on 8% gels.

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    Anti Decorin (DCN) mAb (Clone 889C7)

    Catalog No.(s): CAC-PRPG-DC-M01

    Catalog numbers beginning with "CAC" are antibodies from our exclusive Cosmo Bio Antibody Collection. Visit the CAC Antibody homepage to browse the collection list, organized by research topic. Click here to browse a well organized list of...

    $524.00
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