Signal Transduction

  • Immunoblot analysis. Rib cartilage extract (2.5 μg protein) from 4-week-old rat was subjected to immunoblot analysis using bChM-6 antibody at 500-fold dilution. ChM-1 is detected as a diffuse band of 20-25 kDa due to variation in glycosylation. This antibody also detects the N-terminal glycosylation domain-deleted form of ChM-1(14 kDa ChM-1).

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    Anti Chondromodulin-I (ChM-I) mAb (Clone bCHM-6)

    Catalog No.(s): CAC-TCS-006

    Catalog numbers beginning with "CAC" are antibodies from our exclusive Cosmo Bio Antibody Collection. Visit the CAC Antibody homepage to browse the collection list, organized by research topic.Click here to browse a well organized list of...

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  • Immunostaining of mouse cartilage.

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    Anti Chondromodulin-I (ChM-I) mAb (Clone hCHM-5)

    Catalog No.(s): CAC-TCS-005

    Catalog numbers beginning with "CAC" are antibodies from our exclusive Cosmo Bio Antibody Collection. Visit the CAC Antibody homepage to browse the collection list, organized by research topic.Click here to browse a well organized list of...

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  • Recombinant human ChM-1 (5 ng) was subjected to immunoblot analysis using hChM-3 antibody at 1/500 dilution.

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    Anti Chondromodulin-I (ChM-I) mAb (Clone hCHM-4)

    Catalog No.(s): CAC-TCS-004

    Catalog numbers beginning with "CAC" are antibodies from our exclusive Cosmo Bio Antibody Collection. Visit the CAC Antibody homepage to browse the collection list, organized by research topic.Click here to browse a well organized list of...

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  • Anti Chondromodulin-I (ChM-I) mAb (Clone hCHM-3)

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    Anti Chondromodulin-I (ChM-I) mAb (Clone hCHM-3)

    Catalog No.(s): CAC-TCS-003

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  • Recombinant human ChM-1 (5 ng) was subjected to immunoblot analysis using hChM-2 antibody at 1/100 dilution.

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    Anti Chondromodulin-I (ChM-I) mAb (Clone hCHM-2)

    Catalog No.(s): CAC-TCS-002

    Catalog numbers beginning with "CAC" are antibodies from our exclusive Cosmo Bio Antibody Collection. Visit the CAC Antibody homepage to browse the collection list, organized by research topic.Click here to browse a well organized list of...

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  • Anti Chondromodulin-I (ChM-I) mAb (Clone hCHM-1)

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    Anti Chondromodulin-I (ChM-I) mAb (Clone hCHM-1)

    Catalog No.(s): CAC-TCS-001

    Catalog numbers beginning with "CAC" are antibodies from our exclusive Cosmo Bio Antibody Collection. Visit the CAC Antibody homepage to browse the collection list, organized by research topic.Click here to browse a well organized list of...

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  • Anti Filamin-A (FLN-A) pAb (Rabbit, Antiserum)

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    Anti Filamin-A (FLN-A) pAb (Rabbit, Antiserum)

    Catalog No.(s): CAC-SU-IZ-P05

    Catalog numbers beginning with "CAC" are antibodies from our exclusive Cosmo Bio Antibody Collection. Visit the CAC Antibody homepage to browse the collection list, organized by research topic.Click here to browse a well organized list of...

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  • Figure 4. ELISA of C32 melanoma cell exosomes with anti-CD9 (12A12-ALP) antibody (5 ng/mL).

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    Anti CD9 Antigen (MRP-1/Tspan-29) mAb (Clone 12A12)

    Catalog No.(s): CAC-SHI-EXO-M01-50, CAC-SHI-EXO-M01

    Catalog numbers beginning with "CAC" are antibodies from our exclusive Cosmo Bio Antibody Collection. Visit the CAC Antibody homepage to browse the collection list, organized by research topic.Click here for more information and to see all...

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  • Anti Septin-5 - phospho Ser17 pAb (Rabbit, Affinity Purified)

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    Anti Septin-5 - phospho Ser17 pAb (Rabbit, Affinity Purified)

    Catalog No.(s): CAC-SDT-02-SP5

    Catalog numbers beginning with "CAC" are antibodies from our exclusive Cosmo Bio Antibody Collection. Visit the CAC Antibody homepage to browse the collection list, organized by research topic.Click here for the Neuroscience Products Dashboard...

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  • Figure 1. Western blotting of purified human aorta versican (primarily a mixture of V1 and V2 isoforms) resolved by SDS-PAGE on 3-8% linear gradient gels under reducing conditions (MW, HiMark Unstained Protein Standard) before (Intact) and after combined chondroitinase ABC and endo-b-galactosidase pretreatment (Digested). * Banding pattern observed by immunoblotting depends upon the isoforms and is often complex. In the intact forms, i.e. without removal of GAGs, V0, V1 and V2 do not enter acrylamide gels and therefore agarose gels are recommended. Following chondroitinase-digestion and extensive enzymatic deglycosylation, most isoforms still show complex, smeared banding patterns.

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    Anti Versican (VCAN) mAb (Clone 6B10)

    Catalog No.(s): CAC-PRPG-VS-M04

    Catalog numbers beginning with "CAC" are antibodies from our exclusive Cosmo Bio Antibody Collection. Visit the CAC Antibody homepage to browse the collection list, organized by research topic.Click here to browse a well organized list of...

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  • Figure 1. Western blotting of purified human aorta versican resolved by SDS-PAGE on 3-8% linear gradient gels (MW, HiMark Unstained Protein Standard) before (Intact) and after combined chondroitinase ABC and endo-b-galactosidase pretreatment (Digested). * Banding pattern observed by immunoblotting depends upon the isoforms and is often complex. In the intact forms, i.e. without removal of GAGs, V0, V1 and V2 do not enter acrylamide gels and therefore agarose gels are recommended. Following chondroitinase-digestion and extensive enzymatic deglycosylation, most isoforms still show complex, smeared banding patterns.

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    Anti Versican (VCAN) mAb (Clone 2B3)

    Catalog No.(s): CAC-PRPG-VS-M03

    Catalog numbers beginning with "CAC" are antibodies from our exclusive Cosmo Bio Antibody Collection. Visit the CAC Antibody homepage to browse the collection list, organized by research topic.Click here to browse a well organized list of...

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  • Figure 1. Immunoblotting of intact versican (mixture of V1 and V2 isoforms) prior to (Intact) and after combined chondroitinase ABC (Chase ABC) and endo-galactosidase-digestion (Digested). The proteoglycan was resolved by SDS-PAGE under reducing conditions on 3-8% linear gradient gels (MW, HiMark Unstained Protein Standard). Banding pattern depends upon the isoforms and is often complex. In the intact forms, i.e. without removal of GAGs, isoforms V0, V1 and V2 do not enter conventional polyacrylamide gels and therefore alternative gel types are strongly recommended. Following chondroitinase-digestion and extensive enzymatic deglycosylation, most isoforms still show complex, smeared banding patterns.

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    Anti Versican/CSPG2 (VCAN) mAb (Clone 4C5)

    Catalog No.(s): CAC-PRPG-VS-M02

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  • Figure 1. Immunoblotting of intact versican (mixture of V1 and V2 isoforms) in its untreated and chondroitinase ABC (Chase ABC)-digested form, or after combined digestion with chondroitinase ABC and a number of exo- and endoglycosydases. The proteoglycan was resolved by SDS-PAGE under reducing conditions on 3-8% linear gradient gels (MW, HiMark Unstained Protein Standard).

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    Anti Versican/CSPG2 (VCAN) mAb (Clone 5C12)

    Catalog No.(s): CAC-PRPG-VS-M01

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  • Immunostaining of malignant glioblastoma A) and cutaneous melanoma C) lesions. B) Western blot of whole tissue/cell lysates of a human glioblastoma lesion and the human melanoma A375 cell line after SDS-PAGE under reducing conditions on 4-10% gradient gels. D) Immunostaining of human melanoma MeWo cells. Nuclear counterstaining was performed with Hoescht.

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    Anti NG2 (CSPG4) mAb (Clone 2161D7)

    Catalog No.(s): CAC-PRPG-NG-M01

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  • Figure 1. Immunoprecipitation of biotinylated laminin-nidogen complexes resolved by SDS-PAGE on 5% gels under reducing conditions and visualized by chemiluminescence; red arrow points to the nidogen band, whereas upper bands correspond to a, b and g laminin chains.

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    Anti Laminin/Nidogen complex mAb (Clone 331G3)

    Catalog No.(s): CAC-PRPG-NDG-M01

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  • Anti Laminin Alpha-4 mAb (Clone 652C4)

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    Anti Laminin Alpha-4 mAb (Clone 652C4)

    Catalog No.(s): CAC-PRPG-LA4-M01

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  • Immunolabelling of human adult skin. B) Immunoblot analysis of sarcoma HT1080 whole-cell extracts; C) Flow cytometric analysis of A375 melanoma cells.

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    Anti Integrin Alpha-6 (VLA-6) mAb (Clone 537D5)

    Catalog No.(s): CAC-PRPG-ITG-M01

    Catalog numbers beginning with "CAC" are antibodies from our exclusive Cosmo Bio Antibody Collection. Visit the CAC Antibody homepage to browse the collection list, organized by research topic.Click here to browse a well organized list of...

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  • Figure 1. Western blotting on intact (left) and chondroitinase ABC-digested (right) bovine decorin after SDS-PAGE on 8% gels.

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    Anti Decorin (DCN) mAb (Clone 889C7)

    Catalog No.(s): CAC-PRPG-DC-M01

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  • Figure 1. Characterization of the polyclonal anti-N-syndecan antiserum. Western blot using anti-N-syndecan of a partially purified preparation of membrane-bound proteoglycan (lane 1), and that digested with chondroitinase ABC (lane 2), and heparitinase I (lane 3). After the heparitinase I treatment , the immunoreactive band is sifted to an apparent molecular weight of approximately 140 kDa, identical to that of the core glycoprotein of rat N-syndecan.

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    Anti N-Syndecan (SDC3) pAb

    Catalog No.(s): CAC-NU-07-004

    Visit our Neurodegeneration Products homepage to browse related research productsClick here to browse a well organized list of products for Bone, Collagen, and Extracellular Matrix research.Catalog numbers beginning with "CAC" are antibodies...

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  • Figure 1. Western blot analysis. Whole cell lysates prepared from DJM-1 cells (lane 1) and HeLa cells (lane 2) were separated by SDS-PAGE (5% gel) and immunoblotted with PN753 or PC742 (1: 200 dilution). Plectin antibodies detected approximately 500 kDa sized bands (arrow). The smaller polypeptide found in lane 2 may be a degraded product or alternatively spliced ​​rod-less isoform of plectin.

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    Anti Plectin (PCN/PLTN) mAb (Clone PN753)

    Catalog No.(s): CAC-NU-01-PLN

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  • Figure 1. Western Blot Analysis of extracellular matrix made from Human keratinocyte cell line HaCaT using Anti-Laminin alpha 3 (clone BM515). Arrows indicate Laminin alpha 3 (approximately 160kDa). Non-processed form of Laminin α3 chain might be detected around 190kDa depending on the specimen.

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    Anti Laminin Subunit Alpha-3 mAb (Clone BM515)

    Catalog No.(s): CAC-NU-01-LA3

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  • Figure 1. Western Blotting. Concentrated conditioned medium prepared from BMGE+H cells was immunoblotted with BML39 antibody (1:50 dilution). BML39 antibody detected type-7 collagen as a band at approximately 300 kDa (full-length) and degraded fragments around 170 kDa Conditioned medium was concentrated by ammonia sulfate precipitation as described previously (ref. 2). Polypeptides were separated by SDS-PAGE (7.5%).

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    Anti Collagen Alpha-1(VII) Chain mAb (Clone BML39)

    Catalog No.(s): CAC-NU-01-CO7

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  • Figure 1. WB analysis. Whole cell lysate prepared from DJM-1 cells was immunoblotted with mAb 279 at 1:100 dilution. mAb 279 recognized BPAG1 (Bullous Pemphigoid antigen 1) as a band at approximately 230 kDa (arrow).

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    Anti Dystonin (BPAG1/BP230) mAb (Clone 279)

    Catalog No.(s): CAC-NU-01-BP1

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  • Immunofluorescence analysis of monoclonal antibody (E39) recognition of gamma-tubulin at centrosomes. Gamma-tubulin in normal or cancer cells was stained by immunofluorescence described below. The presence of supernumerary centrosomes is a common event in human tumors. Yellow and blue indicate gamma-tubulin and nuclear DNA, respectively. A: normal human fibroblasts. B: colon cancer cell line (CaCo-2). C: fibrosarcoma cell line (HT-1080).

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    Anti Tubulin Gamma-1 Chain (GCP-1) mAb (Clone E39)

    Catalog No.(s): CAC-NM-MA-003

    Click here to browse a well organized list of products for Bone, Collagen, and Extracellular Matrix research.Catalog numbers beginning with "CAC" are antibodies from our exclusive Cosmo Bio Antibody Collection. Visit the CAC Antibody homepage...

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  • Figure 1. CD44 is a single-pass type I transmembrane protein that functions as a cellular adhesion molecule for hyaluronic acid, a major component of the extracellular matrix. It exists in numerous isoforms that are generated through alternative splicing of CD44 precursor mRNA.

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    Anti CD44 Antigen v9 mAb (Clone RV3)

    Catalog No.(s): CAC-LKG-M003, CAC-LKG-M001

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