VCPIP CD(25-561) [GST-tagged]

Ubiquigent

Catalog No: UBI-64-0055-050

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Species Reactivity: human  
Source: E.coli  
Formula: 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol  
Concentration: 0.5 mg/ml  
Molecular Weight: ~87kDa  
Protein Sequence: Accession number: NP_079330. For full protein sequence information download the Certificate of Analysis pdf.  
Quality Control Protein Identification: Confirmed by mass spectrometry.  
Quality Control Activity: Deubiquitylase Enzyme Assay: The activity of GST-VCPIP was validated by determining the increase in fluorescence measured as a result of the enzyme catalysed cleavage of the fluorogenic substrate Ubiquitin-Rhodamine110-Glycine generating Ubiquitin and Rhodamine110-Glycine. Incubation of the substrate in the presence or absence of GST-VCPIP was compared confirming the deubiquitylating activity of GST-VCPIP.