USP8 [untagged]

Ubiquigent

Catalog No: UBI-64-0053-050

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Species Reactivity: human  
Source: E.coli  
Formula: 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol   
Concentration: 0.5 mg/ml  
Molecular Weight: ~128kDa  
Protein Sequence: Accession number: NP_005145. For full protein sequence information download the Certificate of Analysis pdf.  
Quality Control Protein Identification: Confirmed by mass spectrometry.  
Quality Control Activity: Deubiquitylase Enzyme Assay: The activity of USP8 was validated by determining the increase in fluorescence measured as a result of the enzyme catalysed cleavage of the fluorogenic substrate Ubiquitin-Rhodamine110-Glycine generating Ubiquitin and Rhodamine110-Glycine. Incubation of the substrate in the presence or absence of USP8 was compared confirming the deubiquitylating activity of USP8.