UCHL1 [6His-tagged]

Ubiquigent

Catalog No: UBI-64-0007-050

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Species Reactivity: Human  

Source: E. coli expression  

Formula: 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol  

Concentration: 0.5 mg/ml  

Molecular Weight: ~27 kDa  

Protein Sequence: Accession number: AAH00332.1. For full protein sequence information download the Certificate of Analysis pdf.  

Quality Control Protein Identification: Confirmed by mass spectrometry.  

Quality Control Activity: Deubiquitylating Enzyme Assay:  The activity of His-UCHL1 was validated by determining the increase in fluorescence measured as a result of the enzyme catalysed cleavage of the fluorogenic substrate Ubiquitin-Rhodamine110-Glycine generating Ubiquitin and Rhodamine110-Glycine. Incubation of the substrate in the presence or absence of His-UCHL1 was compared confirming the deubiquitylating activity of His-UCHL1.