UBE2R2 (CDC34B) [untagged]

Ubiquigent

Catalog No: UBI-62-0098-020

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Species Reactivity: Human  
Source: E. coli expression  
Formula: 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol  
Concentration: 1 mg/ml  
Molecular Weight: ~29 kDa  
Protein Sequence: Accession number: AAH04862. For full protein sequence information download the Certificate of Analysis pdf.  
Quality Control Protein Identification: Confirmed by mass spectrometry.  
uality Control Activity: E2-Ubiquitin Thioester Loading Assay: The activity of UBE2R2 was validated by loading E1 UBE1 activated ubiquitin onto the active cysteine of the UBE2R2 E2 enzyme via a transthiolation reaction. Incubation of the UBE1 and UBE2R2 enzymes in the presence of ubiquitin and ATP at 30 °C was compared at two time points, T0 and T10 minutes. Sensitivity of the ubiquitin/UBE2R2 thioester bond to the reducing agent DTT was confirmed.   

Background

The enzymes of the ubiquitylation pathway play a pivotal role in a number of cellular processes including the regulated and targeted proteasomal degradation of substrate proteins. Three classes of enzymes are involved in the process of ubiquitylation; activating enzymes (E1s), conjugating enzymes (E2s) and protein ligases (E3s). UBE2R2 is a member of the E2 conjugating enzyme family and cloning of the human gene was first described by Semplici et al. (2002). Site directed mutagenesis studies have shown that serine 233 in the C-terminal domain of UBE2R2 is the site at which CK2-dependent phosphorylation occurs (Semplici et al., 2002). In vitro binding experiments have also demonstrated that phosphorylated UBE2R2 and UBE2R1 bind specifically to the F-box protein beta-TRCP, which results in enhanced degradation of beta-catenin (a substrate of the Beta Transducin Repeat Containing protein (BTRC) (Semplici et al., 2002).

Reference:

Semplici F, Meggio F, Pinna LA, Oliviero S (2002) CK2-dependent phosphorylation of the E2 ubiquitin conjugating enzyme UBC3B induces its interaction with beta-TrCP and enhances beta-catenin degradation. Oncogene 21, 3978-87.