Tetramer/BV421 - H-2 Kb Toxoplasma gondii tgd057 (SVLAFRRL)

MBL

Catalog No: MBL-TC-5103-4

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Anti-mouse CD8 clone choice is critical when mouse MHC I tetramers, as some clones, including 53-6.7, lead to high background staining of all CD8+ T cells, rather than the antigen-specific ones. For best results, clone KT15, available from MBL International in FITC (K0227-4)is recommended. Additional information can be found in the datasheet that accompanies mouse class I tetramers, as well as the following reference containing a protocol for performing a cross titration series for optimization: Current Protocols in Immunology, MHC-Peptide tetramers to Visualize Antigen-Specific T Cells, Section 17.3.23-24, Supplement 103

Product Specifications

• Application: FC
• Reactivity: Mouse
• Conjugation: BV421
• Peptide Sequence: SVLAFRRL
• Class: Class I
• MHC Allele: H-2K^b