Anti-mouse CD8 clone choice is critical when mouse MHC I tetramers, as some clones, including 53-6.7, lead to high background staining of all CD8+ T cells, rather than the antigen-specific ones. For best results, clone KT15, available from MBL International in FITC (K0227-4)is recommended. Additional information can be found in the datasheet that accompanies mouse class I tetramers, as well as the following reference containing a protocol for performing a cross titration series for optimization: Current Protocols in Immunology, MHC-Peptide tetramers to Visualize Antigen-Specific T Cells, Section 17.3.23-24, Supplement 221
| Product Specifications | |
| Application | FC |
| Reactivity | mouse |
| Conjugation | PE |
| Peptide Sequence | GYKDGNEYI |
| Citations for T-Select H-2Kd Listeria LLO Tetramer-GYKDGNEYI – 2 Found |
| Hayashi, Toshiyuki; Nagai, Shigenori; Fujii, Hideki; Baba, Yukiko; Ikeda, Eiji; Kawase, Takeshi; Koyasu, Shigeo. Critical roles of NK and CD8+ T cells in central nervous system listeriosis. Journal Of Immunology (Baltimore, Md. : 1950). 2009;182(10):6360-8. PubMed |
| Ozawa, Yuichi; Suda, Takafumi; Nagata, Toshi; Hashimoto, Dai; Nakamura, Yutaro; Enomoto, Noriyuki; Inui, Naoki; Koide, Yukio; Nakamura, Hirotoshi; Chida, Kingo. Mucosal vaccine using CTL epitope-pulsed dendritic cell confers protection for intracellular pathogen. American Journal Of Respiratory Cell And Molecular Biology. 2009;41(4):440-8. PubMed |