Anti-mouse CD8 clone choice is critical when mouse MHC I tetramers, as some clones, including 53-6.7, lead to high background staining of all CD8+ T cells, rather than the antigen-specific ones. For best results, clone KT15, available from MBL International in FITC (K0227-4)is recommended. Additional information can be found in the datasheet that accompanies mouse class I tetramers, as well as the following reference containing a protocol for performing a cross titration series for optimization: Current Protocols in Immunology, MHC-Peptide tetramers to Visualize Antigen-Specific T Cells, Section 17.3.23-24, Supplement 219
| Product Specifications | |
| Application | FC |
| Reactivity | mouse |
| Conjugation | PE |
| Peptide Sequence | ASNENMDTM |
| Citations for T-Select H-2Db Influenza NP Tetramer-ASNENMDTM-PE – 3 Found |
| Nakamura, Risa; Maeda, Naoyoshi; Shibata, Kensuke; Yamada, Hisakata; Kase, Tetsuo; Yoshikai, Yasunobu. Interleukin-15 is critical in the pathogenesis of influenza a virus-induced acute lung injury. Journal Of Virology. 2010;84(11):5574-82. PubMed |
| Takada, Kensuke; Van Laethem, Francois; Xing, Yan; Akane, Kazuyuki; Suzuki, Haruhiko; Murata, Shigeo; Tanaka, Keiji; Jameson, Stephen C; Singer, Alfred; Takahama, Yousuke. TCR affinity for thymoproteasome-dependent positively selecting peptides conditions antigen responsiveness in CD8(+) T cells. Nature Immunology. 2015;16(10):1069-76. PubMed |
| Ishikawa, Hiroki; Sasaki, Hiraku; Fukui, Toshie; Fujita, Koji; Kutsukake, Etsuko; Matsumoto, Tetsuya. Mice with asthma are more resistant to influenza virus infection and NK cells activated by the induction of asthma have potentially protective effects. Journal Of Clinical Immunology. 2012;32(2):256-67. PubMed |