T&A Cloning Kit

Yeastern

Catalog No: YSN-FYC001-20P

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Molecular cloning assisted by vectors is the most popular and common method to obtain genes of interest. Yeastern Biotech’s T&A™ Cloning Kit offers a quick, reliable and efficient method for cloning a variety of DNA sequences.

The T&A™ Cloning Kit contains the T&A™ Cloning Vector and all the reagents needed for ligation. It is a convenient pack for cloning PCR product generated using thermostable DNA polymerases, such as YEAtaq DNA polymerase, which add a single terminal 3′-dA nucleotide overhang. After ligation, the mixture can be used directly for transformation into competent cells (ECOS™) or be purified first to achieve higher transformation efficiency.

Features

• Fast ligation, completed in only 5 minutes
• High transformation efficiency
• More accurate results
• Accept a wide range of inserts with different sizes
• Two types of ligation buffers provided for your convenience
• Allow blue/white screening
• Contain ampicillin marker for antibiotic selection
• Include M13 primer sites for convenient sequencing

Applications

• Cloning of terminal 3’-dA nucleotides overhang PCR products up to 5 kb

Quality Control

• DNA concentration of T&A™ Cloning Vector is 25 ng/μl
• The absorbance ratio (A₂₆₀/A₂₈₀) is between 1.6~2.0
• The size of T&A™ Cloning Vector is about 2.7 kb
• The colony number of background control is less than 50 when the transformation efficiency of competent cells is 1 × 10⁸cfu/μg DNA
• The colony number ratio of self-ligation control to positive control is less than 15%
• The colony number of the positive control is more than 500 when the transformation efficiency of competent cells is 5 × 10⁸cfu/μg DNA
• The ligation correctness with the control insert into T&A™ Cloning Vector is more than 87.5%