Background
This spin-column type IgG antibody-purify kit consists of immobilized Protein A (Catalog Number: CSR-APK-10A) or Protein G (Catalog Number: CSR-APK-10G) on the solid phase of filter type monolithic silica with uniform continuous pores. Compared with conventional ones, this product can easily purify IgG antibodies in a shorter time (approximately 3 minutes). The column can be recycled up to 5 times without diminishing antibody binding capacity. Each can purify IgG antibodies up to 0.4 mg with Protein A and 0.3 mg with Protein G per column under more than 90% of recovery rate. 1: In case purified antibodies or culture supernatants are used, up to five replicates of the columns can be reused. However, if the sample contains a solid component such as protein in the serum, it may cause a clogging of the column, which may result in a decrease in the regeneration frequency and fluid permeability. Therefore, this product should be used after removal of the solid component by centrifugal removal or filter filtration.
Composition
Kit contains sufficient reagents for 60 individual purifications (approx. 3 runs per column)
| Component | Quantity |
| Monolithic silica-based Spin Column | 10 Columns |
| Sampling tube | 10 tubes |
| Binding Buffer | 30 ml |
| Wash Buffer | 30 ml |
| Elution Buffer | 30 ml |
| Neutralization Buffer | 15 ml |
| Regeneration Buffer | 30 ml |
| Column Type | Recommended Sample | |
| Protein G Catalog Number: CSR-APK-10G |
Monoclonal Antibody | Human IgG1, IgG2, IgG3, IgG4 |
| Mouse IgG1, IgG2a, IgG2b, IgG3 | ||
| Polyclonal Antibody | Rabbit, Goat | |
| Protein A Catalog Number: CSR-APK-10A |
Monoclonal Antibody | Human IgG1, IgG2, IgG4 |
| Mouse IgG2a, IgG2b, IgG3 | ||
| Polyclonal Antibody | Rabbit | |
Features
- Easy-to-handle spin columns
- Fast purification process: IgG antibodies can be purified in aprox. 3 minutes!
- Column can be re-used (max. 5 times)
References
Hattori,et al. A small molecule compound with an indole moiety inhibits the main protease of SARS-CoV-2 and blocks virus replication. (2021) Nat Commun.28:12:668.
Oshiro,et al. Development of an immunochromatographic kit to detect severe acute respiratory syndrome coronavirus 2. (2021) J Virol Methods. 294 : 114183.
Protein G
Takamatsu,et al. SARS-CoV-2-neutralizing humoral IgA response occurs earlier but modest and diminishes faster compared to IgG response. (2022) bioRxiv. Jun 9;2022.06.09.495422.
Protein A
Maegawa,et al. Anti-calcitonin gene-related peptide antibody attenuates orofacial mechanical and heat hypersensitivities induced by infraorbital nerve injury. (2021) Biochem Biophys Res Commun.10 ; 569 : 147-153.
| Documents & Links for Spin column-based Antibody Purification Kit (Protein A) | |
| Datasheet | Spin column-based Antibody Purification Kit (Protein A) Datasheet |
| Documents & Links for Spin column-based Antibody Purification Kit (Protein A) | |
| Datasheet | Spin column-based Antibody Purification Kit (Protein A) Datasheet |