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Source: BALB/C Mouse implantation ascites
Formula: 10mM phosphate buffer, 0.15M NaCl PH7.4, 0.1% sodium azide
Concentration: 1 mg/ml
Molecular Weight: 150kDa
Protein Sequence: n/a
Quality Control Protein Identification: n/a
Quality Control Activity: Anti-Polyubiquitylated Conjugates mAb (FK1) Antibody Activity Assay: The activity of anti-polyubiquitylated conjugates mAb (FK1) antibody was tested for its ability to recognise polyubiquitin chains (Lane 3) but not mono-ubiquitin conjugates or free ubiquitin. By Western blotting the specific recognition of polyubiquitin conjugates by the anti-polyubiquitylated conjugates mAb (FK1) over mono-ubiquitin conjugates (Di-ubiquitin) or free ubiquitin was demonstrated. In a priming and extension assay containing, UBE1 [6His-tagged] (Cat# 61-0001), UBE2W [6His-tagged] (Cat# 62-0085), UBE2N [untagged] (Cat# 62-0047), UBE2V1 [untagged] (Cat# 62-0059), Ubiquitin (Cat# 60-0001), CHIP [untagged] (Cat# 63-0003) and ATP. Using the antipolyubiquitylated conjugates mAb (FK1) antibody, detection of polyubiquitin chains extending from mono-ubiquitylated CHIP and free chains generated by UBE2N/UBE2V1 in the presence of CHIP were observed.
Background
The anti-polyubiquitylated conjugates mAb (FK1) demonstrates specific recognition for polyubiquitin protein conjugates but shows no reactivity with monoubiquitylated proteins or free ubiquitin (Fujimoro et al. 1994). The antipolyubiquitylated conjugates mAb (FK1) has been extensively characterised and used not only to investigate polyubiquitin chain formation on proteins by Western blotting but also in the detection of intracellular polyubiquitin chains in immunoassays (Takada et al. 1995; Fujimoro et al. 2005).
References
Fujimuro M, Sawada H, Yokosawa H (1994) Production and characterization of monoclonal antibodies specific to multiubiquitin chains of polyubiquitinated proteins. FEBS Lett 349 173-180.
Takada K, Nasu H, Hibi N, Tsukada Y, Ohkawa K, Fujimuro M,Sawada H, Yokosawa H (1995) Immunoassay for the quantification of intracellular multi-ubiquitin chains. Eur J Biochem 233 42-47.
Fujimuro M, Yokosawa H (2005) Production of antipolyubiquitin monoclonal antibodies and their use for characterization and isolation of polyubiquitinated proteins. Methods Enzymol 399 75-86.
| Product Specifications | |
| Application | WB |
| Reactivity | Human |
| Clonality | Monoclonal (Clone No.: FK1) |
| Host | Mouse |
| Source | Monoclonal |
| Conjugation | Monoclonal |
| Documents & Links for Polyubiquitylated conjugates, mAb (FK1) | |
| Datasheet | ubi-68-0111-500_polyubiquitylated-conjugates-mab-fk1_datasheet.pdf |
| Vendor Page | Polyubiquitylated conjugates, mAb (FK1) at Ubiquigent |
| Documents & Links for Polyubiquitylated conjugates, mAb (FK1) | |
| Datasheet | ubi-68-0111-500_polyubiquitylated-conjugates-mab-fk1_datasheet.pdf |
| Vendor Page | Polyubiquitylated conjugates, mAb (FK1) |