pFucci-S/G2/M Green-Hyg (Expression vector)

MBL

Catalog No: MBL-AM-V9010M

FUCCI (Fluorescent Ubiquitination-based Cell Cycle Indicator) is a set of fluorescent probes which enables the visualization of cell cycle progression in living cells. FUCCI utilizes the phase-dependent nature of replication licensing factors Cdt1 and Geminin. A fusion protein of a fragment of Cdt1 (amino acids 30-120) with the fluorescent protein monomeric Kusabira-Orange 2 (mKO2) serves as an indicator of G1 phase. A fusion protein of a fragment of Geminin (amino acids 1-110 or 1-60) with the fluorescent protein monomeric Azami-Green 1 (mAG1) visualizes the S,G2 and M phase. In summary, FUCCI utilizes the highly selective, rapid degradation of the replication licensing factors mediated by the ubiquitin proteasome system to give excellent visualizations of the cell cycle.

Citations for pFucci-S/G2/M Green-Hyg (Expression vector) – 32 Found

Newman, Robert H; Zhang, Jin. Fucci: street lights on the road to mitosis. Chemistry & Biology. 2008;15(2):97-8. 

Sugiyama, Mayu; Sakaue-Sawano, Asako; Iimura, Tadahiro; Fukami, Kiyoko; Kitaguchi, Tetsuya; Kawakami, Koichi; Okamoto, Hitoshi; Higashijima, Shin-ichi; Miyawaki, Atsushi. Illuminating cell-cycle progression in the developing zebrafish embryo. Proceedings Of The National Academy Of Sciences Of The United States Of America. 2009;106(49):20812-7. 

Morikawa, Hanako; Kim, Minsoo; Mimuro, Hitomi; Punginelli, Claire; Koyama, Tomohiro; Nagai, Shinya; Miyawaki, Atsushi; Iwai, Kazuhiro; Sasakawa, Chihiro. The bacterial effector Cif interferes with SCF ubiquitin ligase function by inhibiting deneddylation of Cullin1. Biochemical And Biophysical Research Communications. 2010;401(2):268-74. 

Ogura, Yosuke; Sakaue-Sawano, Asako; Nakagawa, Masashi; Satoh, Nori; Miyawaki, Atsushi; Sasakura, Yasunori. Coordination of mitosis and morphogenesis: role of a prolonged G2 phase during chordate neurulation. Development (Cambridge, England). 2011;138(3):577-87. 

Yang, Liangpeng; Kotomura, Naoe; Ho, Yik-Khuan; Zhi, Huijun; Bixler, Sandra; Schell, Michael J; Giam, Chou-Zen. Complex cell cycle abnormalities caused by human T-lymphotropic virus type 1 Tax. Journal Of Virology. 2011;85(6):3001-9. 

Malka, Yuval; Eden, Sharon. Analysis of apoptosis in FUCCI HeLa cells. Cytometry. Part A : The Journal Of The International Society For Analytical Cytology. 2011;79(4):243-6. 

Tofighi, Roshan; Wan Ibrahim, Wan Norhamidah; Rebellato, Paola; Andersson, Patrik L; Uhlén, Per; Ceccatelli, Sandra. Non-dioxin-like polychlorinated biphenyls interfere with neuronal differentiation of embryonic neural stem cells. Toxicological Sciences : An Official Journal Of The Society Of Toxicology. 2011;124(1):192-201. 

Kaida, Atsushi; Miura, Masahiko. Visualizing the effect of hypoxia on fluorescence kinetics in living HeLa cells using the fluorescent ubiquitination-based cell cycle indicator (Fucci). Experimental Cell Research. 2012;318(3):288-97. 

Dan, Shingo; Okamura, Mutsumi; Mukai, Yumiko; Yoshimi, Hisashi; Inoue, Yasumichi; Hanyu, Aki; Sakaue-Sawano, Asako; Imamura, Takeshi; Miyawaki, Atsushi; Yamori, Takao. ZSTK474, a specific phosphatidylinositol 3-kinase inhibitor, induces G1 arrest of the cell cycle in vivo. European Journal Of Cancer (Oxford, England : 1990). 2012;48(6):936-43. 

Downey, Mike J; Jeziorska, Danuta M; Ott, Sascha; Tamai, T Katherine; Koentges, Georgy; Vance, Keith W; Bretschneider, Till. Extracting fluorescent reporter time courses of cell lineages from high-throughput microscopy at low temporal resolution. Plos One. 6(12):e27886. 

Ridenour, Dennis A; McKinney, Mary Cathleen; Bailey, Caleb M; Kulesa, Paul M. CycleTrak: a novel system for the semi-automated analysis of cell cycle dynamics. Developmental Biology. 2012;365(1):189-95. 

Kaida, Atsushi; Miura, Masahiko. Differential dependence on oxygen tension during the maturation process between monomeric Kusabira Orange 2 and monomeric Azami Green expressed in HeLa cells. Biochemical And Biophysical Research Communications. 2012;421(4):855-9. 

Ito, Sayuri; Ishii, Ai; Kakusho, Naoko; Taniyama, Chika; Yamazaki, Satoshi; Fukatsu, Rino; Sakaue-Sawano, Asako; Miyawaki, Atsushi; Masai, Hisao. Mechanism of cancer cell death induced by depletion of an essential replication regulator. Plos One. 7(5):e36372. 

Hashimoto, T; Juso, K; Nakano, M; Nagano, T; Kambayashi, S; Nakashima, A; Kikkawa, U; Kamada, S. Preferential Fas-mediated apoptotic execution at G1 phase: the resistance of mitotic cells to the cell death. Cell Death & Disease. 2012;3(5):e313. 

Abe, Takaya; Sakaue-Sawano, Asako; Kiyonari, Hiroshi; Shioi, Go; Inoue, Ken-ichi; Horiuchi, Toshitaka; Nakao, Kazuki; Miyawaki, Atsushi; Aizawa, Shinichi; Fujimori, Toshihiko. Visualization of cell cycle in mouse embryos with Fucci2 reporter directed by Rosa26 promoter. Development (Cambridge, England). 2013;140(1):237-46. 

Coronado, Diana; Godet, Murielle; Bourillot, Pierre-Yves; Tapponnier, Yann; Bernat, Agnieszka; Petit, Maxime; Afanassieff, Marielle; Markossian, Suzy; Malashicheva, Anna; Iacone, Roberto; Anastassiadis, Konstantinos; Savatier, Pierre. A short G1 phase is an intrinsic determinant of naïve embryonic stem cell pluripotency. Stem Cell Research. 2013;10(1):118-31. 

Roccio, Marta; Schmitter, Daniel; Knobloch, Marlen; Okawa, Yuya; Sage, Daniel; Lutolf, Matthias P. Predicting stem cell fate changes by differential cell cycle progression patterns. Development (Cambridge, England). 2013;140(2):459-70. 

Laurent, Jennifer; Frongia, Céline; Cazales, Martine; Mondesert, Odile; Ducommun, Bernard; Lobjois, Valérie. Multicellular tumor spheroid models to explore cell cycle checkpoints in 3D. Bmc Cancer. 2013;13:73. 

Equilibrina, I; Matsunaga, S; Morimoto, A; Hashimoto, T; Uchiyama, S; Fukui, K. ASURA (PHB2) interacts with Scc1 through chromatin. Cytogenetic And Genome Research. 139(4):225-33. 

Bao, Yan; Mukai, Kuniaki; Hishiki, Takako; Kubo, Akiko; Ohmura, Mitsuyo; Sugiura, Yuki; Matsuura, Tomomi; Nagahata, Yoshiko; Hayakawa, Noriyo; Yamamoto, Takehiro; Fukuda, Ryo; Saya, Hideyuki; Suematsu, Makoto; Minamishima, Yoji Andrew. Energy management by enhanced glycolysis in G1-phase in human colon cancer cells in vitro and in vivo. Molecular Cancer Research : Mcr. 2013;11(9):973-85. 

Nagano, Taiki; Hashimoto, Toshiaki; Nakashima, Akio; Hisanaga, Shin-ichi; Kikkawa, Ushio; Kamada, Shinji. Cyclin I is involved in the regulation of cell cycle progression. Cell Cycle (Georgetown, Tex.). 2013;12(16):2617-24. 

Bouchard, G; Bouvette, G; Therriault, H; Bujold, R; Saucier, C; Paquette, B. Pre-irradiation of mouse mammary gland stimulates cancer cell migration and development of lung metastases. British Journal Of Cancer. 2013;109(7):1829-38. 

Tomura, Michio; Sakaue-Sawano, Asako; Mori, Yoshiko; Takase-Utsugi, Mitsuyo; Hata, Akihiro; Ohtawa, Kenji; Kanagawa, Osami; Miyawaki, Atsushi. Contrasting quiescent G0 phase with mitotic cell cycling in the mouse immune system. Plos One. 8(9):e73801. 

Pauklin, Siim; Vallier, Ludovic. The cell-cycle state of stem cells determines cell fate propensity. Cell. 2013;155(1):135-47. 

Sakaue-Sawano, Asako; Hoshida, Tetsushi; Yo, Masahiro; Takahashi, Reiko; Ohtawa, Kenji; Arai, Takashi; Takahashi, Eiki; Noda, Shinichi; Miyoshi, Hiroyuki; Miyawaki, Atsushi. Visualizing developmentally programmed endoreplication in mammals using ubiquitin oscillators. Development (Cambridge, England). 2013;140(22):4624-32. 

Singh, Amar M; Chappell, James; Trost, Robert; Lin, Li; Wang, Tao; Tang, Jie; Matlock, Brittany K; Weller, Kevin P; Wu, Hao; Zhao, Shaying; Jin, Peng; Dalton, Stephen. Cell-cycle control of developmentally regulated transcription factors accounts for heterogeneity in human pluripotent cells. Stem Cell Reports. 1(6):532-44. 

Paladino, Patrick; Marcon, Edyta; Greenblatt, Jack; Frappier, Lori. Identification of herpesvirus proteins that contribute to G1/S arrest. Journal Of Virology. 2014;88(8):4480-92. 

Dowling, Mark R; Kan, Andrey; Heinzel, Susanne; Zhou, Jie H S; Marchingo, Julia M; Wellard, Cameron J; Markham, John F; Hodgkin, Philip D. Stretched cell cycle model for proliferating lymphocytes. Proceedings Of The National Academy Of Sciences Of The United States Of America. 2014;111(17):6377-82. 

Nahar, Kamrun; Goto, Tatsuaki; Kaida, Atsushi; Deguchi, Shifumi; Miura, Masahiko. Effects of Chk1 inhibition on the temporal duration of radiation-induced G2 arrest in HeLa cells. Journal Of Radiation Research. 2014;55(5):1021-7. 

Carlier, Géraldine; Maugein, Alicia; Cordier, Corinne; Pechberty, Séverine; Garfa-Traoré, Meriem; Martin, Patrick; Scharfmann, Raphaël; Albagli, Olivier. Human fucci pancreatic Beta cell lines: new tools to study Beta cell cycle and terminal differentiation. Plos One. 9(9):e108202. 

Jin, Sha; Cordes, Nils. ATM controls DNA repair and mitochondria transfer between neighboring cells. Cell Communication And Signaling : Ccs. 2019;17(1):144. 

Matsumoto, Yasunori; Kano, Masayuki; Murakami, Kentaro; Toyozumi, Takeshi; Suito, Hiroshi; Takahashi, Masahiko; Sekino, Nobufumi; Shiraishi, Tadashi; Kamata, Toshiki; Ryuzaki, Takahiro; Hirasawa, Soichiro; Kinoshita, Kazuya; Matsubara, Hisahiro. Tumor-derived exosomes influence the cell cycle and cell migration of human esophageal cancer cell lines. Cancer Science. 2020;111(12):4348-4358.