OTUD1 CD(270-481) [6His-tagged]

Ubiquigent

Catalog No: UBI-64-0054-050

Click here to view the Ubiquigent^TM Product Dashboard and hundreds of great reagents for ubiquitin-proteasome-related research.

Customer Notice Regarding Orders for Ubiquigent Products: From September 2020, orders for Ubiquigent products must total at least $700 to enable prompt delivery. Orders less than this amount may be subject to unpredictable wait times. Please contact us for further details.

Species Reactivity: human  
Source: E.coli  
Formula: 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol  
Concentration: 0.5 mg/ml  
Molecular Weight: ~30kDa  
Protein Sequence: Accession number: NP_001138845. For full protein sequence information download the Certificate of Analysis pdf.  
Quality Control Protein Identification: Confirmed by mass spectrometry.  
Quality Control Activity: Deubiquitylase Enzyme Assay: The activity of His-OTUD1 was validated by determining the increase in fluorescence measured as a result of the enzyme catalysed cleavage of the fluorogenic substrate Ubiquitin-Rhodamine110-Glycine generating Ubiquitin and Rhodamine110-Glycine. Incubation of the substrate in the presence or absence of His-OTUD1 was compared confirming the deubiquitylating activity of His-OTUD1.