Three-dimensional culture of blood vessel endothelium using the collagen gel (Eguchi R, Hyogo College of Medicine Department of Environmental and Preventive Medicine) An immunosuppressant FK506 is known to be involved in endothelial dysfunction inducing thrombotic microangiopathy after hematopoietic stem cell transplantation. In order to clarify the mechanism of the event, human umbilical vein endothelial cells (HUVEC) in collagen gel 3D culture were treated with FK506. In 3D culture, FK506 induced cell death and tube structure breakdown in a time- and concentration-dependent manner, but showed little effect on cells in monolayer culture. These results suggest 3D culture in collagen gel is useful to investigate in vivo phenomenon in vitro (Ref.6).

Native collagen, Bovine dermis, 3mg/mL

Catalog No:
KOU-IAC-30
$267.00

ATTENTION US CUSTOMERS: We will fully subsidize the import permit of qualified first-time users. FREE samples may be available for 50% shipping fee. Contact us for information about permits and samples.

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Background
Highly purified collagen solution (pH 3.0) for cell culture. Suitable for cell cultureware coating and preparing collagen gels. The low pH of the Acid Solutions enables long term refrigerated storage. In contrast to cell-derived soluble basement membrane preparations, Collagen Solutions are free from bioactive substances, nucreic acids, MMP, etc. allowing experimental results to be evaluated more clearly.

Applications

  • Collagen coating for cell cultureware
  • Cell culture in a collagen gel
  • Cell culture on a collagen gel

Features

  • Native collagen gelates when neutralized and placed under physiological condition.
  • Ready-to-use: atelocollagen is premixed with medium

This product is manufactured in Japan and produced from raw materials obtained from livestock certified born and raised in Australia or New Zealand. Japan, Australia and New Zealand are all recognized by the World Health Organization as having negligible risk for Bovine Spongiform Encephalitis (BSE). Nevertheless, please consult with your country's Customs office to confirm importability.

Documents & Links for Native collagen, Bovine dermis, 3mg/mL
Datasheet Native collagen, Bovine dermis, 3mg/mL Datasheet
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Vendor Page Native collagen, Bovine dermis, 3mg/mL at Cosmo Bio LTD

Documents & Links for Native collagen, Bovine dermis, 3mg/mL
Datasheet Native collagen, Bovine dermis, 3mg/mL Datasheet
Flyer Native collagen, Bovine dermis, 3mg/mL Flyer
Vendor Page Native collagen, Bovine dermis, 3mg/mL

Citations for Native collagen, Bovine dermis, 3mg/mL – 32 Found
ECM microenvironment unlocks brown adipogenic potential of adult human bone marrow-derived MSCs.
Sci Rep. 2016 Feb 17;6:21173. doi: 10.1038/srep21173.  PubMed
Relatively Rare Populations of Invasive Cells Drive Progression of Heterogeneous Tumors.
Cell Mol Bioeng. 2024 Jan 5;17(1):7-24. doi: 10.1007/s12195-023-00792-w. eCollection 2024 Feb.  PubMed
- Comments: IAC 3D culture
Somatostatin affects GnRH neuronal development and migration and stimulates olfactory-related fiber fasciculation.
Dev Neurobiol. 2024 Jan;84(1):3-17. doi: 10.1002/dneu.22931. Epub 2023 Dec 10.  PubMed
- Comments: IAC 3D culture
β-adrenergic receptor agonist promotes ductular expansion during 3,5-diethoxycarbonyl-1,4-dihydrocollidine-induced chronic liver injury.
Sci Rep. 2023 May 1;13(1):7084. doi: 10.1038/s41598-023-33882-w.  PubMed
- Comments: IAC 3D culture
The HEDGEHOG-GLI1 pathway is important for fibroproliferative properties in keloids and as a candidate therapeutic target.
Commun Biol. 2023 Dec 7;6(1):1235. doi: 10.1038/s42003-023-05561-z.  PubMed
- Comments: IAC 3D culture
Synthetic Collagen-like Polymer That Undergoes a Sol-Gel Transition Triggered by O-N Acyl Migration at Physiological pH.
Int J Mol Sci. 2022 Jan 29;23(3):1584. doi: 10.3390/ijms23031584.  PubMed
- Comments: IAC 3D culture
Therapeutic Potential of Adipose Stem Cell-Derived Conditioned Medium on Scar Contraction Model.
Biomedicines. 2022 Sep 24;10(10):2388. doi: 10.3390/biomedicines10102388.  PubMed
- Comments: IAC 3D culture
Three-Dimensional Cell Drawing Technique in Hydrogel Using Micro Injection System.
Micromachines (Basel). 2022 Oct 30;13(11):1866. doi: 10.3390/mi13111866.  PubMed
- Comments: IAC 3D culture
Anti-skin-aging effects of human ceramides via collagen and fibrillin expression in dermal fibroblasts.
Biosci Biotechnol Biochem. 2022 Aug 24;86(9):1240-1246. doi: 10.1093/bbb/zbac107.  PubMed
- Comments: IAC 3D culture
STIM-Orai1 signaling regulates fluidity of cytoplasm during membrane blebbing.
Nat Commun. 2021 Jan 20;12(1):480. doi: 10.1038/s41467-020-20826-5.  PubMed
- Comments: IAC 3D culture
Travelling ultrasound promotes vasculogenesis of three-dimensional-monocultured human umbilical vein endothelial cells.
Biotechnol Bioeng. 2021 Oct;118(10):3760-3769. doi: 10.1002/bit.27852. Epub 2021 Jun 16.  PubMed
- Comments: IAC 3D culture
Matrix degradation and cell proliferation are coupled to promote invasion and escape from an engineered human breast microtumor.
Integr Biol (Camb). 2021 Feb 3;13(1):17-29. doi: 10.1093/intbio/zyaa026.  PubMed
- Comments: IAC 3D culture
Two-photon microscopic observation of cell-production dynamics in the developing mammalian neocortex in utero.
Dev Growth Differ. 2020 Feb;62(2):118-128. doi: 10.1111/dgd.12648. Epub 2020 Jan 14.  PubMed
- Comments: IAC 3D culture
Rapid Photoinduced Single Cell Detachment from Gold Nanoparticle-Embedded Collagen Gels with Low Denaturation Temperature.
Polymers (Basel). 2020 Jan 15;12(1):213. doi: 10.3390/polym12010213.  PubMed
- Comments: IAC 3D culture
Interstitial Hypertension Suppresses Escape of Human Breast Tumor Cells Via Convection of Interstitial Fluid.
Cell Mol Bioeng. 2020 Nov 9;14(2):147-159. doi: 10.1007/s12195-020-00661-w. eCollection 2021 Apr.  PubMed
- Comments: IAC 3D culture
Hydrostatic pressure promotes endothelial tube formation through aquaporin 1 and Ras-ERK signaling.
Commun Biol. 2020 Apr 2;3(1):152. doi: 10.1038/s42003-020-0881-9.  PubMed
- Comments: IAC 3D culture
A crucial role for Arf6 in the response of commissural axons to Slit.
Development. 2019 Feb 4;146(3):dev172106. doi: 10.1242/dev.172106.  PubMed
- Comments: IAC 3D culture
N(ω) -(Carboxymethyl)arginine Is One of the Dominant Advanced Glycation End Products in Glycated Collagens and Mouse Tissues.
Oxid Med Cell Longev. 2019 Sep 10;2019:9073451. doi: 10.1155/2019/9073451. eCollection 2019.  PubMed
- Comments: IAC 3D culture
Effect of Mechanical Compression on Invasion Process of Malignant Melanoma Using In Vitro Three-Dimensional Cell Culture Device.
Micromachines (Basel). 2019 Sep 30;10(10):666. doi: 10.3390/mi10100666.  PubMed
- Comments: IAC 3D culture
Effect of cell culture density on dental pulp-derived mesenchymal stem cells with reference to osteogenic differentiation.
Sci Rep. 2019 Apr 1;9(1):5430. doi: 10.1038/s41598-019-41741-w.  PubMed
- Comments: IAC 3D culture
Downregulation of ERG and FLI1 expression in endothelial cells triggers endothelial-to-mesenchymal transition.
PLoS Genet. 2018 Nov 30;14(11):e1007826. doi: 10.1371/journal.pgen.1007826. eCollection 2018 Nov.  PubMed
- Comments: IAC 3D culture
Elasticity-based boosting of neuroepithelial nucleokinesis via indirect energy transfer from mother to daughter.
PLoS Biol. 2018 Apr 20;16(4):e2004426. doi: 10.1371/journal.pbio.2004426. eCollection 2018 Apr.  PubMed
- Comments: IAC 3D culture
Differentiating cells mechanically limit the interkinetic nuclear migration of progenitor cells to secure apical cytogenesis.
Development. 2018 Jul 17;145(14):dev162883. doi: 10.1242/dev.162883.  PubMed
- Comments: IAC 3D culture
Both triazolyl ester of ketorolac (15K) and YM155 inhibit the embryonic angiogenesis in ovo (fertilized eggs) via their common PAK1-survivin/VEGF signaling pathway.
Drug Discov Ther. 2017;11(6):300-306. doi: 10.5582/ddt.2017.01058.  PubMed
- Comments: IAC 3D culture
Dysregulated Collagen Homeostasis by Matrix Stiffening and TGF-β1 in Fibroblasts from Idiopathic Pulmonary Fibrosis Patients: Role of FAK/Akt.
Int J Mol Sci. 2017 Nov 16;18(11):2431. doi: 10.3390/ijms18112431.  PubMed
- Comments: IAC 3D culture
Dynamics of Tissue-Induced Alignment of Fibrous Extracellular Matrix.
Biophys J. 2017 Aug 8;113(3):702-713. doi: 10.1016/j.bpj.2017.06.046.  PubMed
- Comments: IAC 3D culture
Defined three-dimensional microenvironments boost induction of pluripotency.
Nat Mater. 2016 Mar;15(3):344-52. doi: 10.1038/nmat4536. Epub 2016 Jan 11.  PubMed
- Comments: IAC 3D culture
ECM microenvironment unlocks brown adipogenic potential of adult human bone marrow-derived MSCs.
Sci Rep. 2016 Feb 17;6:21173. doi: 10.1038/srep21173.  PubMed
- Comments: IAC 3D culture
Increased AGE-RAGE ratio in idiopathic pulmonary fibrosis.
Respir Res. 2016 Nov 5;17(1):144. doi: 10.1186/s12931-016-0460-2.  PubMed
- Comments: IAC 3D culture
Interstitial fluid pressure regulates collective invasion in engineered human breast tumors via Snail, vimentin, and E-cadherin.
Integr Biol (Camb). 2016 Mar 14;8(3):319-31. doi: 10.1039/c5ib00282f.  PubMed
- Comments: IAC 3D culture
Mammary Branching Morphogenesis Requires Reciprocal Signaling by Heparanase and MMP-14.
J Cell Biochem. 2015 Aug;116(8):1668-79. doi: 10.1002/jcb.25127.  PubMed
- Comments: IAC 3D culture
Fibroblast viability and phenotypic changes within glycated stiffened three-dimensional collagen matrices.
Respir Res. 2015 Jul 1;16(1):82. doi: 10.1186/s12931-015-0237-z.  PubMed
- Comments: IAC 3D culture