KBM501 (Medium for activation of human T cells, NK cells and Dendritic cells)

Cosmo Bio LTD

Catalog No: KJN-16025015

KBM 501 (Medium for Activation of Human NK Cells)

Osmolality: 290 ± 15 mOsm/kg H₂O (determined by freezing point depression osmometry)
pH: 7.2 ± 0.2 (determined using a glass electrode)
Sterilization: Negative (Membrane filtration)
Mycoplasma Test: Negative (PCR detection)
Endotoxin Test: Less than 0.3 EU/mL (Determined by Limulus amebocyte lysate assay)

Notice: This product is only for research use, and not for human or animal therapeutic use.

 

Cell Culture Examples

Proliferative response of IL-2-dependent human   Cytotoxicity of activated lymphocytes to K562 cells lymphocytes on immobilized anti-CD3 antibody

Performance evaluation of KBM501:
Cell: Normal human peripheral blood monocytes
Vessel:12-wellplateonwhichanti-human-CD3 antibody is immobilized.
Cytokine: Recombinant human interleukin-2(2814IU/mL)
Plasma:1% inactivated autoplasma was added at the onset of culture.

Proliferative test: The culture was started at a seeding densityof27×104 cells/mL, under static conditions at 37℃,5%CO2.
The culture was diluted by adding each medium containing plasma upon cell proliferation.
At day10,cells unstained by trypan blue were counted.

Analysis: At day10,surface markers of activated lymphocytes were analyzed byflowcytometry,andcytotoxicitytoK562cells were measured with TeraScan that is a micro fluoro cytometer developed for measuring cytotoxicity.

Code:16025015
Product Name:KBM501
Form: Liquid
Cytokine: Recombinant human interleukin-2(2814IU/mL)
Size:500mL
Storage:2-8℃
Shelf Life:8 months from production date