KBM 501 (Medium for Activation of Human NK Cells)
Overview
This product is a culture medium suitable for activating human lymphocytes (T cells, NK cells, dendritic cells).
Cytokines appropriate for the target cells should be added at the time of use.
Add 5 to 10% serum (plasma) at the start of activation culture.
It is recommended to use KBM 502 and KBM 502B for the expansion culture after activation.
This product contains recombinant human interleukin-2 (IL-2) at 1750 JRU/mL (2810 IU/mL).
Osmolality: 290 ± 15 mOsm/kg H2O (determined by freezing point depression osmometry)
pH: 7.2 ± 0.2 (determined using a glass electrode)
Sterilization: Negative (Membrane filtration)
Mycoplasma Test: Negative (PCR detection)
Endotoxin Test: Less than 0.3 EU/mL (Determined by Limulus amebocyte lysate assay)
Notice: This product is only for research use, and not for human or animal therapeutic use.
Cell Culture Examples
Proliferative response of IL-2-dependent human Cytotoxicity of activated lymphocytes to K562 cells lymphocytes on immobilized anti-CD3 antibody
Performance evaluation of KBM501:
Cell: Normal human peripheral blood monocytes
Vessel:12-wellplateonwhichanti-human-CD3 antibody is immobilized.
Cytokine: Recombinant human interleukin-2(2814IU/mL)
Plasma:1% inactivated autoplasma was added at the onset of culture.
Proliferative test: The culture was started at a seeding densityof27×104 cells/mL, under static conditions at 37℃,5%CO2.
The culture was diluted by adding each medium containing plasma upon cell proliferation.
At day10,cells unstained by trypan blue were counted.
Analysis: At day10,surface markers of activated lymphocytes were analyzed byflowcytometry,andcytotoxicitytoK562cells were measured with TeraScan that is a micro fluoro cytometer developed for measuring cytotoxicity.
Catalog Number: KJN-16025015
Product Name:KBM501
Form: Liquid
Cytokine: Recombinant human interleukin-2(2814IU/mL)
Size:500mL
Storage:2-8℃
Shelf Life: 8 months from production date
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