KBM Neural Stem Cell (Supplement)

Cosmo Bio LTD

Catalog No: KJN-16050300

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This is the supplement for KBM Neural Stem Cell, a serum-free, chemically-defined complete medium for culturing neural stem cells (NSCs) as neurospheres. It is also capable of inducing differentiation of NSCs into neural cells by adherent cell cultures of neurospheres, without addition of supplement. This supplement DOES NOT INCLUDE 500ml basal medium (KJN-16050100). If you would like to purchase the complete medium, please see KJN-16050200.

KBM Neural Stem Cell Series＋ KBM XB2



Capable of both undifferentiated proliferation and inducing differentiation of NSCs.
(Serum-free medium for Neural stem cells)









KBM Neural Stem Cell




• This medium is specialized for the neurosphere assay which is widely applied research and development regarding neural stem cells (NSCs) and neural cells. By adding KBM Neural Stem Cell Supplement to this medium and proceeding suspension culture, it is capable of culturing NSCs as neurospheres. Cultured neurospheres can produce new neurospheres by dispersing cells and re-culturing. It is also capable of inducing differentiation of NSCs into neural cells by adherent cell cultures of neurospheres, without addition of supplement.
  
  

• A kit based on a test developed by the Japan Fruit Juice Association, detects contamination with Alicyclobacillus acidoterrestris and related Thermo-acidophilic Bacilli (TAB) by their production of guaiacol.








Product Features



【KBM Neural Stem Cell + Supplement】




• Serum-free and chemically-defined

• Capable of both undifferentiated proliferation and inducing differentiation of NSCs.

• Confirmed the capability to culture NSCs derived human iPS cells.




【KBM XB2】




• Chemically-defined spplement(50x concentration)

• Contains retinoic acid(vitaminA)






Culture Examples



(a)PDT variation at passages of NSCs	(b)Total number of NSCs at passages	(c)Picture of cultured neurospheres
(d)Picture of differentiated neurons	(e)Picture of cultured neurospheres



 



	Culture Conditions (a,b,c)	Differentiation Induction Assay(d)	Culture Condition(e)
Culture medium	KBM Neural Stem Cell＋KBM Neural Stem Cell Supplement	KBM Neural Stem Cell	KBM Neural stem cell + KBM XB2
Cell culture vessel	6 well cell culture plate（corning 3471）	Cell culture cover glass Poly-L-ornithine coating	Cell culture cover glass Poly-L-ornithine coating
Protocols	Cells were seeded at 1×105 cells/mL, and passaged every 5 days.	Neurospheres were seeded and cultured for 5 days. Neurospheres were seeded and cultured for 5 days
Antibodies		Green : anti-β tubulin â ¢Blue : anti-GFAPRed : anti-CNPase	Green：TuJ-1



 Product Lineup



Catalog Number	Product name	Size
KJN-16050100	KBM Neural Stem Cell	500mL
KJN-16050300	KBM Neural Stem Cell Supplement	1mL
KJN-16050400	KBM XB2	10mL