IMMUNIS HBV Genotype EIA

Catalog No:
IIM-1A65
$1,845.00
Reactivity: Human 

This product is an enzyme immunoassay (EIA) based HBV genotyping kit, designed to determine genotype (A, B, C, or D) by detecting genotype-specific epitopes in PreS2 region . 
Many studies indicate that HBV genotypes influence the severityof chronic hepatitis B and the response to interferon therapy. HBV genotypes have been determined by tedious methods such as direct sequencing, genotype-specific PCR, PCR-RFLP, line-probe assay (LiPA), or PCR-invader assay.

Background
There are eight genotypes (genotype A to H) of hepatitis B virus (HBV) which are distinguished by > 8 % difference in the nucleotide sequence of the HBV genome. Genotypes A and D are common in Europe and the United States, while genotypes B and C are prevalent in Asia. Genotype F is frequent in Latin America, while genotype E is confined to Central and Western Africa.

Many studies indicate that HBV genotypes influence the severity of chronic hepatitis B and the response to interferon therapy. HBV genotypes have been determined by tedious methods such as direct sequencing, genotype-specific PCR, PCR-RFLP, line-probe assay (LiPA), or PCR-invader assay.

This product is an enzyme immunoassay (EIA) based HBV genotyping kit, designed to determine genotype (A, B, C, or D) by detecting genotype-specific epitopes in PreS2 region .

Feature and Advantages

  • The first diagnostic reagent for determination of HBV genotype launched in the Japanese market
  • Fast and easy detection based on one-step EIA method
  • 98% of samples with HBsAg concentration of >3 IU/mL can be genotyped (unpublished data)
  • Sample volume: 80μL of HBsAg positive serum
  • Stored samples can be used

Application sample
Hepatitis B Virus serological genotyping in human serum (Genotype A, B, C, or D)

Determination of HBV genotypes
Determine genotypes by the positive/negative combinations (serotypes) of four epitopes (‘m’, ‘k’, ‘s’, or ‘u’) in accordance with the table below.Sample sera showing serotypes other than ‘su’, ‘m’, 'ks’, or ‘ksu’ can not be classified by this kit.

PreS2 epitope PreS2 serotype HBV genotype
m k s u
- - + + su A
+ - - - m B
- + + - ks C
- + + + ksu D
        Others Unclassified

Kit Contents

Content Quantity
Microplate coated with anti-HBs (8 wells x12 strips) 2 plates.
HRP-labeled mAbs (HRP-labeled anti-PreS2mouse monoclonal antibodies)
1) HRP-labeled anti-‘m’ mAb (green) 5 mL x 1 vial
2) HRP-labeled anti-‘k’ mAb (yellow) 5 mL x 1 vial
3) HRP-labeled anti-‘s’ mAb (blue) 5 mL x 1 vial
4) HRP-labeled anti-‘u’ mAb (red) 5 mL x 1 vial
Negative control 1 mL x 1 vial
Positive controls
1) Positive control ‘m’ (green) 0.25 mL x 1 vial
2) Positive control ‘k’ (yellow) 0.25 mL x 1 vial
3) Positive control 's’ (blue) 0.25 mL x 1 vial
4) Positive control ‘u’ (red) 0.25 mL x 1 vial
HRP substrate (3, 3', 5,5'-tetramethylbenzidine) 20 mL x 1 vial
Reaction stopper 20 mL x 1 vial
Washing solution (20x concentrated) 50 mL x 2 vials l
Plate seal 6 sheets

References

Documents & Links for IMMUNIS HBV Genotype EIA
Datasheet IMMUNIS HBV Genotype EIA Datasheet
Datasheet IMMUNIS HBV Genotype EIA Datasheet
Vendor Page IMMUNIS HBV Genotype EIA at Cosmo Bio LTD

Documents & Links for IMMUNIS HBV Genotype EIA
Datasheet IMMUNIS HBV Genotype EIA Datasheet
Datasheet IMMUNIS HBV Genotype EIA Datasheet
Vendor Page IMMUNIS HBV Genotype EIA