HEK293 cell lysate (IL-1 stimulated)

Ubiquigent

Catalog No: UBI-66-3001-010

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Species Reactivity: Human  
Source: IL-1-stimulated embryonic kidney (HEK293) cells  
Formula: 50mM Tris/HCl pH7.5, 1mM EGTA, 1mM EDTA, 1% Triton X-100, 1mM sodium orthovanadate, 50mM sodium fluoride, 5mM sodium pyrophosphate, 10mM sodium β-glycerophosphate, 270mM sucrose, 1mM PMSF, 1mM benzamidine, 50mM iodoacetamide.  
Concentration: 20mg/ml  
Molecular Weight: n/a  
Protein Sequence: n/a  
Quality Control Protein Identification: n/a  
Quality Control Activity: Identification of Polyubiquitin Chains in Lysate:  A Western blot of IL-1 stimulated HEK293 cell lysate (lane 2) and non-stimulated HEK293 cell  lysate (lane 3) probed with an anti-FK2 antibody indicates that both of the lysates are abundant  with ubiquitylated proteins and possibly free ubiquitin chains. Polyubiquitin (Ub2-7) (K63-linked)  was included as a positive control in this experiment (lane 1).