Anti-mouse CD8 clone choice is critical when mouse MHC I tetramers, as some clones, including 53-6.7, lead to high background staining of all CD8+ T cells, rather than the antigen-specific ones. For best results, clone KT15, available from MBL International in FITC (K0227-4)is recommended. Additional information can be found in the datasheet that accompanies mouse class I tetramers, as well as the following reference containing a protocol for performing a cross titration series for optimization: Current Protocols in Immunology, MHC-Peptide tetramers to Visualize Antigen-Specific T Cells, Section 17.3.23-24, Supplement 255
| Product Specifications | |
| Application | FC |
| Reactivity | mouse |
| Conjugation | PE |
| Peptide Sequence | QYIHSANVL |
| Citations for H-2Kd Erk1 Tetramer-QYIHSANVL-PE – 2 Found |
| Nishikawa, Hiroyoshi; Kato, Takuma; Hirayama, Michiko; Orito, Yuki; Sato, Eiichi; Harada, Naozumi; Gnjatic, Sacha; Old, Lloyd J; Shiku, Hiroshi. Regulatory T cell-resistant CD8+ T cells induced by glucocorticoid-induced tumor necrosis factor receptor signaling. Cancer Research. 2008;68(14):5948-54. PubMed |
| Ikeda, H; Ohta, N; Furukawa, K; Miyazaki, H; Wang, L; Kuribayashi, K; Old, L J; Shiku, H. Mutated mitogen-activated protein kinase: a tumor rejection antigen of mouse sarcoma. Proceedings Of The National Academy Of Sciences Of The United States Of America. 1997;94(12):6375-9. PubMed |