Description
Product Description
Anti-mouse CD8 clone choice is critical when mouse MHC I tetramers, as some clones, including 53-6.7, lead to high background staining of all CD8+ T cells, rather than the antigen-specific ones. For best results, clone KT15, available from MBL International in FITC (K0227-4)is recommended. Additional information can be found in the datasheet that accompanies mouse class I tetramers, as well as the following reference containing a protocol for performing a cross titration series for optimization: Current Protocols in Immunology, MHC-Peptide tetramers to Visualize Antigen-Specific T Cells, Section 17.3.23-24, Supplement 166
Product Specifications
Product Specifications | |
Application | FC |
Reactivity | Mouse |
Conjugation | PE |
Peptide Sequence | gp33 (C9M) |
Class | Class I |
Sequence Location | 33-41 |
MHC Allele | H-2Db |
Documents & Links
Documents & Links for H-2Db LCMV gp33 (C9M) Tetramer-KAVYNFATM-PE | |
Datasheet | H-2Db LCMV gp33 (C9M) Tetramer-KAVYNFATM-PE Datasheet |
Documents & Links for H-2Db LCMV gp33 (C9M) Tetramer-KAVYNFATM-PE | |
Datasheet | H-2Db LCMV gp33 (C9M) Tetramer-KAVYNFATM-PE Datasheet |
Citations
Citations for H-2Db LCMV gp33 (C9M) Tetramer-KAVYNFATM-PE – 1 Found |
Raju, Saravanan; Kometani, Kohei; Kurosaki, Tomohiro; Shaw, Andrey S; Egawa, Takeshi. The adaptor molecule CD2AP in CD4 T cells modulates differentiation of follicular helper T cells during chronic LCMV infection. Plos Pathogens. 2018;14(5):e1007053. PubMed |