Anti-mouse CD8 clone choice is critical when mouse MHC I tetramers, as some clones, including 53-6.7, lead to high background staining of all CD8+ T cells, rather than the antigen-specific ones. For best results, clone KT15, available from MBL International in FITC (K0227-4)is recommended. Additional information can be found in the datasheet that accompanies mouse class I tetramers, as well as the following reference containing a protocol for performing a cross titration series for optimization: Current Protocols in Immunology, MHC-Peptide tetramers to Visualize Antigen-Specific T Cells, Section 17.3.23-24, Supplement 160
| Product Specifications | |
| Application | FC |
| Reactivity | Mouse |
| Conjugation | PE |
| Peptide Sequence | Nucleoprotein |
| Citations for H-2Db Influenza NP Tetramer-ASNENMETM-PE – 3 Found |
| Seo, Sang-Uk; Kwon, Hyung-Joon; Song, Joo-Hye; Byun, Young-Ho; Seong, Baik Lin; Kawai, Taro; Akira, Shizuo; Kweon, Mi-Na. MyD88 signaling is indispensable for primary influenza A virus infection but dispensable for secondary infection. Journal Of Virology. 2010;84(24):12713-22. PubMed |
| Morimoto, Junko; Sato, Kayoko; Nakayama, Yosuke; Kimura, Chiemi; Kajino, Kiichi; Matsui, Yutaka; Miyazaki, Tadaaki; Uede, Toshimitsu. Osteopontin modulates the generation of memory CD8+ T cells during influenza virus infection. Journal Of Immunology (Baltimore, Md. : 1950). 2011;187(11):5671-83. PubMed |
| Uematsu, Takayuki; Iizasa, Ei'ichi; Kobayashi, Noritada; Yoshida, Hiroki; Hara, Hiromitsu. Loss of CARD9-mediated innate activation attenuates severe influenza pneumonia without compromising host viral immunity. Scientific Reports. 2015;5:17577. PubMed |